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When NERVE (New Enhanced RV Environment), the first RV software integrating tools to perform the selection of VCs, was released, it prompted further development in the field. However, the problem-solving potential of most, if not all, RV programs is still largely unexploited by experimental vaccinologists that impaired by somehow difficult interfaces, requiring bioinformatic skills.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We report here on the development and release of NERVE 2.0 (available at:\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/nerve-bio.org\" ext-link-type=\"uri\">https:\/\/nerve-bio.org<\/jats:ext-link>\n                      ) which keeps the original integrative and modular approach of NERVE, while showing higher predictive performance than its previous version and other web-RV programs (Vaxign and Vaxijen). We renewed some of its modules and added innovative ones, such as\n                      <jats:italic>Loop-Razor<\/jats:italic>\n                      , to recover fragments of promising vaccine candidates or\n                      <jats:italic>Epitope\u00a0Prediction<\/jats:italic>\n                      for the epitope prediction binding affinities and population coverage. Along with two newly built AI (Artificial Intelligence)-based models:\n                      <jats:italic>ESPAAN<\/jats:italic>\n                      and\n                      <jats:italic>Virulent<\/jats:italic>\n                      . To improve user-friendliness, NERVE was shifted to a tutored, web-based interface, with a noSQL-database to consent the user to submit, obtain and retrieve analysis results at any moment.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>With its redesigned and updated environment, NERVE 2.0 allows customisable and refinable bacterial protein vaccine analyses to all different kinds of users.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12859-024-06004-0","type":"journal-article","created":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T06:49:48Z","timestamp":1734504588000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["NERVE 2.0: boosting the new enhanced reverse vaccinology environment via artificial intelligence and a user-friendly web interface"],"prefix":"10.1186","volume":"25","author":[{"given":"Andrea","family":"Conte","sequence":"first","affiliation":[]},{"given":"Nicola","family":"Gulmini","sequence":"additional","affiliation":[]},{"given":"Francesco","family":"Costa","sequence":"additional","affiliation":[]},{"given":"Matteo","family":"Cartura","sequence":"additional","affiliation":[]},{"given":"Felix","family":"Br\u00f6hl","sequence":"additional","affiliation":[]},{"given":"Francesco","family":"Patan\u00e8","sequence":"additional","affiliation":[]},{"given":"Francesco","family":"Filippini","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2024,12,18]]},"reference":[{"issue":"5","key":"6004_CR1","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1016\/S1369-5274(00)00119-3","volume":"3","author":"R Rappuoli","year":"2000","unstructured":"Rappuoli R. 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