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DiscMycoVir is a pipeline of established tools for next-generation sequencing analysis and database searching, incorporated in an interface that facilitates accessibility even for users that have no programming skills and expertise. A comprehensive and detailed result report enhances user experience. DiscMycoVir can be accessed online for reviewing purposes at: <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/discmycovir.imslab.gr:8000\" ext-link-type=\"uri\">https:\/\/discmycovir.imslab.gr:8000<\/jats:ext-link> and the source code is located at <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/github.com\/abompotas\/DiscMycoVir\" ext-link-type=\"uri\">https:\/\/github.com\/abompotas\/DiscMycoVir<\/jats:ext-link>. We recommend using the GitHub repository, as the online platform may lack the necessary resources to ensure uninterrupted service especially on large files.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods\u2013results<\/jats:title>\n            <jats:p>We employed state-of-the-art technologies in the design and implementation phase of the platform. We present the application of the platform in screening RNA-seq data from the yeast <jats:italic>Candida auris<\/jats:italic> for mycoviruses, demonstrating its efficiency and simplicity in use.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>DiscMycoVir serves as a user-friendly platform for identifying mycoviruses in RNA-seq data. Our tool was successfully implemented to discover mycoviruses in a <jats:italic>C. auris<\/jats:italic> isolate and could be adapted to detect viruses in transcriptomes from other organisms as well.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/s12859-025-06196-z","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T12:52:52Z","timestamp":1751892772000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["DiscMycoVir: a user-friendly platform for discovering mycoviruses in fungal transcriptomes"],"prefix":"10.1186","volume":"26","author":[{"given":"Agorakis","family":"Bompotas","sequence":"first","affiliation":[]},{"given":"Nikitas-Rigas","family":"Kalogeropoulos","sequence":"additional","affiliation":[]},{"given":"Maria","family":"Giachali","sequence":"additional","affiliation":[]},{"given":"Ioly","family":"Kotta-Loizou","sequence":"additional","affiliation":[]},{"given":"Christos","family":"Makris","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2025,7,7]]},"reference":[{"issue":"5","key":"6196_CR1","doi-asserted-by":"publisher","first-page":"e02027-20","DOI":"10.1128\/mBio.02851-20","volume":"11","author":"JM Myers","year":"2020","unstructured":"Myers JM, Bonds AE, Clemons RA, Thapa NA, Simmons DR, Carter-House D, et al. 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