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The development of this pipeline was motivated by the observation of contraction waves in the extra-embryonic membranes of the red flour beetle\n                      <jats:italic>Tribolium castaneum<\/jats:italic>\n                      . These contraction waves are a novel finding, whose origin and function are not yet understood. The objective of the proposed approach is to analyze the dynamics of the extra-embryonic membranes in order to provide quantitative evidence for the existence of contraction waves during late stages of embryonic development.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We apply the pipeline to live-imaging data of\n                      <jats:italic>Tribolium<\/jats:italic>\n                      embryonic development recorded with light-sheet fluorescence microscopy. The proposed pipeline integrates particle image velocimetry (PIV) for quantitative movement analysis, surface detection, tissue cartography, and algorithmic identification of characteristic movement dynamics. We demonstrate that our approach reliably and efficiently detects contraction waves in both 2D+t and 3D+t recordings and enables automated quantitative analyses, such as measuring the area involved in contractile behavior, wave duration and frequency, spatiotemporal location of the contractile regions, and their relation to the underlying velocity distribution.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The pipeline will be employed in future work to conduct a large-scale characterization and quantification of contraction wave behavior in\n                      <jats:italic>Tribolium<\/jats:italic>\n                      development and can be readily adapted for the identification and segmentation of characteristic tissue dynamics in other biological systems.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12859-025-06259-1","type":"journal-article","created":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T08:13:22Z","timestamp":1761034402000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Spatiotemporal segmentation of contraction waves in the extra-embryonic membranes of the red flour beetle"],"prefix":"10.1186","volume":"26","author":[{"given":"Marc","family":"Pereyra","sequence":"first","affiliation":[]},{"given":"Mariia","family":"Golden","sequence":"additional","affiliation":[]},{"given":"Zo\u00eb","family":"Lange","sequence":"additional","affiliation":[]},{"given":"Artemiy","family":"Golden","sequence":"additional","affiliation":[]},{"given":"Frederic","family":"Strobl","sequence":"additional","affiliation":[]},{"given":"Ernst H. 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