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QStrain provides versatile visualization modules such as frequency profiles, conservation scores, RNA expression patterns, and phylogenetic trees to enhance the interpretation of viral sequence variation. By identifying highly conserved genomic regions, QStrain supports the prediction of candidate therapeutic targets and enables the automated design of small interfering RNA (siRNA) and antisense oligonucleotides (ASOs). Additional features include off-target searching and 2D structure viewing for comprehensive assessment of therapeutic potential.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Ultimately, QStrain (\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/qstrain.sookmyung.ac.kr\" ext-link-type=\"uri\">https:\/\/qstrain.sookmyung.ac.kr<\/jats:ext-link>\n                      ) provides a critical, user-friendly framework to accelerate the design pipeline of nucleic acid-based therapies, enhancing preparedness against current and future viral outbreaks.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12859-026-06424-0","type":"journal-article","created":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T11:05:03Z","timestamp":1775819103000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["QStrain: an interactive platform for viral genome analysis and nucleic acid therapeutic design"],"prefix":"10.1186","volume":"27","author":[{"given":"Euna","family":"Jeong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seungyean","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumin","family":"Jeong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hansaem","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung-Chang","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joo-Yeon","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minwook","family":"Shin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukjoon","family":"Yoon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,4,10]]},"reference":[{"key":"6424_CR1","doi-asserted-by":"crossref","unstructured":"Aatresh A, Lurie N, Hatchett R. 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