{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T03:20:41Z","timestamp":1772853641061,"version":"3.50.1"},"reference-count":62,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2014,11,18]],"date-time":"2014-11-18T00:00:00Z","timestamp":1416268800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Syst Biol"],"published-print":{"date-parts":[[2014,12]]},"DOI":"10.1186\/s12918-014-0128-9","type":"journal-article","created":{"date-parts":[[2014,11,17]],"date-time":"2014-11-17T20:02:46Z","timestamp":1416254566000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["Photo-sensitive degron variants for tuning protein stability by light"],"prefix":"10.1186","volume":"8","author":[{"given":"Svetlana","family":"Usherenko","sequence":"first","affiliation":[]},{"given":"Hilke","family":"Stibbe","sequence":"additional","affiliation":[]},{"given":"Massimiliano","family":"Musc\u00f3","sequence":"additional","affiliation":[]},{"given":"Lars-Oliver","family":"Essen","sequence":"additional","affiliation":[]},{"given":"Ekaterina A","family":"Kostina","sequence":"additional","affiliation":[]},{"given":"Christof","family":"Taxis","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2014,11,18]]},"reference":[{"key":"128_CR1","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1038\/nmeth.f.326","volume":"8","author":"JE Toettcher","year":"2011","unstructured":"Toettcher JE, Voigt CA, Weiner OD, Lim WA: The promise of optogenetics in cell biology: interrogating molecular circuits in space and time. Nat Methods. 2011, 8: 35-38. 10.1038\/nmeth.f.326.","journal-title":"Nat Methods"},{"key":"128_CR2","doi-asserted-by":"publisher","first-page":"864","DOI":"10.1126\/science.1235038","volume":"340","author":"C Baarlink","year":"2013","unstructured":"Baarlink C, Wang H, Grosse R: Nuclear actin network assembly by formins regulates the SRF coactivator MAL. Science. 2013, 340: 864-867. 10.1126\/science.1235038.","journal-title":"Science"},{"key":"128_CR3","doi-asserted-by":"publisher","first-page":"973","DOI":"10.1038\/nmeth.1524","volume":"7","author":"MJ Kennedy","year":"2010","unstructured":"Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL: Rapid blue-light-mediated induction of protein interactions in living cells. Nat Methods. 2010, 7: 973-975. 10.1038\/nmeth.1524.","journal-title":"Nat Methods"},{"key":"128_CR4","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1038\/nature04405","volume":"438","author":"A Levskaya","year":"2005","unstructured":"Levskaya A, Chevalier AA, Tabor JJ, Simpson ZB, Lavery LA, Levy M, Davidson EA, Scouras A, Ellington AD, Marcotte EM, Voigt CA: Synthetic biology: engineering Escherichia coli to see light. Nature. 2005, 438: 441-442. 10.1038\/nature04405.","journal-title":"Nature"},{"key":"128_CR5","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1146\/annurev-cellbio-100109-104051","volume":"27","author":"G Miesenbock","year":"2011","unstructured":"Miesenbock G: Optogenetic control of cells and circuits. Annu Rev Cell Dev Biol. 2011, 27: 731-758. 10.1146\/annurev-cellbio-100109-104051.","journal-title":"Annu Rev Cell Dev Biol"},{"key":"128_CR6","doi-asserted-by":"publisher","first-page":"1041","DOI":"10.1038\/nbt734","volume":"20","author":"S Shimizu-Sato","year":"2002","unstructured":"Shimizu-Sato S, Huq E, Tepperman JM, Quail PH: A light-switchable gene promoter system. Nat Biotechnol. 2002, 20: 1041-1044. 10.1038\/nbt734.","journal-title":"Nat Biotechnol"},{"key":"128_CR7","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1186\/1754-1611-3-15","volume":"3","author":"O Sorokina","year":"2009","unstructured":"Sorokina O, Kapus A, Terecskei K, Dixon LE, Kozma-Bognar L, Nagy F, Millar AJ: A switchable light-input, light-output system modelled and constructed in yeast. J Biol Eng. 2009, 3: 15-10.1186\/1754-1611-3-15.","journal-title":"J Biol Eng"},{"key":"128_CR8","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1038\/nature08241","volume":"461","author":"YI Wu","year":"2009","unstructured":"Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn KM: A genetically encoded photoactivatable Rac controls the motility of living cells. Nature. 2009, 461: 104-108. 10.1038\/nature08241.","journal-title":"Nature"},{"key":"128_CR9","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1016\/j.chembiol.2012.02.006","volume":"19","author":"OI Lungu","year":"2012","unstructured":"Lungu OI, Hallett RA, Choi EJ, Aiken MJ, Hahn KM, Kuhlman B: Designing photoswitchable peptides using the AsLOV2 domain. Chem Biol. 2012, 19: 507-517. 10.1016\/j.chembiol.2012.02.006.","journal-title":"Chem Biol"},{"key":"128_CR10","doi-asserted-by":"publisher","first-page":"1105","DOI":"10.1021\/bc3001326","volume":"23","author":"B Schierling","year":"2012","unstructured":"Schierling B, Pingoud A: Controlling the DNA cleavage activity of light-inducible chimeric endonucleases by bidirectional photoactivation. Bioconjug Chem. 2012, 23: 1105-1109. 10.1021\/bc3001326.","journal-title":"Bioconjug Chem"},{"key":"128_CR11","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1038\/nmeth.1904","volume":"9","author":"D Strickland","year":"2012","unstructured":"Strickland D, Lin Y, Wagner E, Hope CM, Zayner J, Antoniou C, Sosnick TR, Weiss EL, Glotzer M: TULIPs: tunable, light-controlled interacting protein tags for cell biology. Nat Methods. 2012, 9: 379-384. 10.1038\/nmeth.1904.","journal-title":"Nat Methods"},{"key":"128_CR12","doi-asserted-by":"publisher","first-page":"10709","DOI":"10.1073\/pnas.0709610105","volume":"105","author":"D Strickland","year":"2008","unstructured":"Strickland D, Moffat K, Sosnick TR: Light-activated DNA binding in a designed allosteric protein. Proc Natl Acad Sci U S A. 2008, 105: 10709-10714. 10.1073\/pnas.0709610105.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"128_CR13","doi-asserted-by":"publisher","first-page":"14001","DOI":"10.1021\/bi701543e","volume":"46","author":"AS Halavaty","year":"2007","unstructured":"Halavaty AS, Moffat K: N- and C-terminal flanking regions modulate light-induced signal transduction in the LOV2 domain of the blue light sensor phototropin 1 from Avena sativa. Biochemistry. 2007, 46: 14001-14009. 10.1021\/bi701543e.","journal-title":"Biochemistry"},{"key":"128_CR14","doi-asserted-by":"publisher","first-page":"1316","DOI":"10.1107\/S1744309113029199","volume":"69","author":"AS Halavaty","year":"2013","unstructured":"Halavaty AS, Moffat K: Coiled-coil dimerization of the LOV2 domain of the blue-light photoreceptor phototropin 1 from Arabidopsis thaliana. Acta Crystallogr Sect F: Struct Biol Cryst Commun. 2013, 69: 1316-1321. 10.1107\/S1744309113029199.","journal-title":"Acta Crystallogr Sect F: Struct Biol Cryst Commun"},{"key":"128_CR15","doi-asserted-by":"publisher","first-page":"16184","DOI":"10.1021\/bi048092i","volume":"43","author":"SM Harper","year":"2004","unstructured":"Harper SM, Christie JM, Gardner KH: Disruption of the LOV-Jalpha helix interaction activates phototropin kinase activity. Biochemistry. 2004, 43: 16184-16192. 10.1021\/bi048092i.","journal-title":"Biochemistry"},{"key":"128_CR16","doi-asserted-by":"publisher","first-page":"1541","DOI":"10.1126\/science.1086810","volume":"301","author":"SM Harper","year":"2003","unstructured":"Harper SM, Neil LC, Gardner KH: Structural basis of a phototropin light switch. Science. 2003, 301: 1541-1544. 10.1126\/science.1086810.","journal-title":"Science"},{"key":"128_CR17","first-page":"1093","volume":"2011","author":"M Terazima","year":"1814","unstructured":"Terazima M: Studies of photo-induced protein reactions by spectrally silent reaction dynamics detection methods: applications to the photoreaction of the LOV2 domain of phototropin from Arabidopsis thaliana. Biochim Biophys Acta. 1814, 2011: 1093-1105.","journal-title":"Biochim Biophys Acta"},{"key":"128_CR18","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1021\/bi101665s","volume":"50","author":"BD Zoltowski","year":"2011","unstructured":"Zoltowski BD, Gardner KH: Tripping the light fantastic: blue-light photoreceptors as examples of environmentally modulated protein-protein interactions. Biochemistry. 2011, 50: 4-16. 10.1021\/bi101665s.","journal-title":"Biochemistry"},{"key":"128_CR19","doi-asserted-by":"publisher","first-page":"827","DOI":"10.1038\/nchembio.210","volume":"5","author":"BD Zoltowski","year":"2009","unstructured":"Zoltowski BD, Vaccaro B, Crane BR: Mechanism-based tuning of a LOV domain photoreceptor. Nat Chem Biol. 2009, 5: 827-834. 10.1038\/nchembio.210.","journal-title":"Nat Chem Biol"},{"key":"128_CR20","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.jmb.2012.02.037","volume":"419","author":"JP Zayner","year":"2012","unstructured":"Zayner JP, Antoniou C, Sosnick TR: The amino-terminal helix modulates light-activated conformational changes in AsLOV2. J Mol Biol. 2012, 419: 61-74. 10.1016\/j.jmb.2012.02.037.","journal-title":"J Mol Biol"},{"key":"128_CR21","doi-asserted-by":"publisher","first-page":"9310","DOI":"10.1021\/bi700852w","volume":"46","author":"JM Christie","year":"2007","unstructured":"Christie JM, Corchnoy SB, Swartz TE, Hokenson M, Han IS, Briggs WR, Bogomolni RA: Steric interactions stabilize the signaling state of the LOV2 domain of phototropin 1. Biochemistry. 2007, 46: 9310-9319. 10.1021\/bi700852w.","journal-title":"Biochemistry"},{"key":"128_CR22","doi-asserted-by":"crossref","unstructured":"Diensthuber RP, Engelhard C, Lemke N, Gleichmann T, Ohlendorf R, Bittl R, Moglich A: Biophysical, Mutational, and Functional Investigation of the Chromophore-Binding Pocket of Light-Oxygen-Voltage Photoreceptors.ACS Synth Biol 2014. Doi: 10.1021\/sb400205x,","DOI":"10.1021\/sb400205x"},{"key":"128_CR23","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1016\/j.jmb.2012.01.056","volume":"417","author":"F Circolone","year":"2012","unstructured":"Circolone F, Granzin J, Jentzsch K, Drepper T, Jaeger KE, Willbold D, Krauss U, Batra-Safferling R: Structural basis for the slow dark recovery of a full-length LOV protein from Pseudomonas putida. J Mol Biol. 2012, 417: 362-374. 10.1016\/j.jmb.2012.01.056.","journal-title":"J Mol Biol"},{"key":"128_CR24","doi-asserted-by":"publisher","first-page":"378","DOI":"10.1021\/bi3015373","volume":"52","author":"KS Conrad","year":"2013","unstructured":"Conrad KS, Bilwes AM, Crane BR: Light-induced subunit dissociation by a light-oxygen-voltage domain photoreceptor from Rhodobacter sphaeroides. Biochemistry. 2013, 52: 378-391. 10.1021\/bi3015373.","journal-title":"Biochemistry"},{"key":"128_CR25","doi-asserted-by":"publisher","first-page":"10321","DOI":"10.1021\/bi901115z","volume":"48","author":"K Jentzsch","year":"2009","unstructured":"Jentzsch K, Wirtz A, Circolone F, Drepper T, Losi A, Gartner W, Jaeger KE, Krauss U: Mutual exchange of kinetic properties by extended mutagenesis in two short LOV domain proteins from Pseudomonas putida. Biochemistry. 2009, 48: 10321-10333. 10.1021\/bi901115z.","journal-title":"Biochemistry"},{"key":"128_CR26","doi-asserted-by":"publisher","first-page":"5346","DOI":"10.1021\/ja1097379","volume":"133","author":"S Raffelberg","year":"2011","unstructured":"Raffelberg S, Mansurova M, Gartner W, Losi A: Modulation of the photocycle of a LOV domain photoreceptor by the hydrogen-bonding network. J Am Chem Soc. 2011, 133: 5346-5356. 10.1021\/ja1097379.","journal-title":"J Am Chem Soc"},{"key":"128_CR27","doi-asserted-by":"publisher","first-page":"8771","DOI":"10.1021\/bi200976a","volume":"50","author":"BD Zoltowski","year":"2011","unstructured":"Zoltowski BD, Nash AI, Gardner KH: Variations in protein-flavin hydrogen bonding in a light, oxygen, voltage domain produce non-Arrhenius kinetics of adduct decay. Biochemistry. 2011, 50: 8771-8779. 10.1021\/bi200976a.","journal-title":"Biochemistry"},{"key":"128_CR28","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1038\/nmeth.1473","volume":"7","author":"D Strickland","year":"2010","unstructured":"Strickland D, Yao X, Gawlak G, Rosen MK, Gardner KH, Sosnick TR: Rationally improving LOV domain-based photoswitches. Nat Methods. 2010, 7: 623-626. 10.1038\/nmeth.1473.","journal-title":"Nat Methods"},{"key":"128_CR29","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1038\/nchembio.99","volume":"4","author":"X Yao","year":"2008","unstructured":"Yao X, Rosen MK, Gardner KH: Estimation of the available free energy in a LOV2-J alpha photoswitch. Nat Chem Biol. 2008, 4: 491-497. 10.1038\/nchembio.99.","journal-title":"Nat Chem Biol"},{"key":"128_CR30","doi-asserted-by":"publisher","first-page":"9901","DOI":"10.1074\/jbc.M111.324723","volume":"287","author":"Y Aihara","year":"2012","unstructured":"Aihara Y, Yamamoto T, Okajima K, Yamamoto K, Suzuki T, Tokutomi S, Tanaka K, Nagatani A: Mutations in N-terminal flanking region of blue light-sensing light-oxygen and voltage 2 (LOV2) domain disrupt its repressive activity on kinase domain in the Chlamydomonas phototropin. J Biol Chem. 2012, 287: 9901-9909. 10.1074\/jbc.M111.324723.","journal-title":"J Biol Chem"},{"key":"128_CR31","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1021\/cb400755b","volume":"9","author":"KM Bonger","year":"2014","unstructured":"Bonger KM, Rakhit R, Payumo AY, Chen JK, Wandless TJ: General method for regulating protein stability with light. ACS Chem Biol. 2014, 9: 111-115. 10.1021\/cb400755b.","journal-title":"ACS Chem Biol"},{"key":"128_CR32","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1038\/nature12466","volume":"500","author":"S Konermann","year":"2013","unstructured":"Konermann S, Brigham MD, Trevino AE, Hsu PD, Heidenreich M, Cong L, Platt RJ, Scott DA, Church GM, Zhang F: Optical control of mammalian endogenous transcription and epigenetic states. Nature. 2013, 500: 472-476.","journal-title":"Nature"},{"key":"128_CR33","doi-asserted-by":"publisher","first-page":"534","DOI":"10.1016\/j.jmb.2012.01.001","volume":"416","author":"R Ohlendorf","year":"2012","unstructured":"Ohlendorf R, Vidavski RR, Eldar A, Moffat K, Moglich A: From dusk till dawn: one-plasmid systems for light-regulated gene expression. J Mol Biol. 2012, 416: 534-542. 10.1016\/j.jmb.2012.01.001.","journal-title":"J Mol Biol"},{"key":"128_CR34","doi-asserted-by":"publisher","first-page":"16480","DOI":"10.1021\/ja3065667","volume":"134","author":"LR Polstein","year":"2012","unstructured":"Polstein LR, Gersbach CA: Light-inducible spatiotemporal control of gene activation by customizable zinc finger transcription factors. J Am Chem Soc. 2012, 134: 16480-16483. 10.1021\/ja3065667.","journal-title":"J Am Chem Soc"},{"key":"128_CR35","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1016\/j.chembiol.2013.03.005","volume":"20","author":"C Renicke","year":"2013","unstructured":"Renicke C, Schuster D, Usherenko S, Essen LO, Taxis C: A LOV2 domain-based optogenetic tool to control protein degradation and cellular function. Chem Biol. 2013, 20: 619-626. 10.1016\/j.chembiol.2013.03.005.","journal-title":"Chem Biol"},{"key":"128_CR36","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1038\/sj.emboj.7601476","volume":"26","author":"J Takeuchi","year":"2007","unstructured":"Takeuchi J, Chen H, Coffino P: Proteasome substrate degradation requires association plus extended peptide. EMBO J. 2007, 26: 123-131. 10.1038\/sj.emboj.7601476.","journal-title":"EMBO J"},{"key":"128_CR37","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1042\/BJ20071239","volume":"410","author":"J Takeuchi","year":"2008","unstructured":"Takeuchi J, Chen H, Hoyt MA, Coffino P: Structural elements of the ubiquitin-independent proteasome degron of ornithine decarboxylase. Biochem J. 2008, 410: 401-407. 10.1042\/BJ20071239.","journal-title":"Biochem J"},{"key":"128_CR38","doi-asserted-by":"publisher","first-page":"21130","DOI":"10.1073\/pnas.1313369110","volume":"110","author":"JB Robertson","year":"2013","unstructured":"Robertson JB, Davis CR, Johnson CH: Visible light alters yeast metabolic rhythms by inhibiting respiration. Proc Natl Acad Sci U S A. 2013, 110: 21130-21135. 10.1073\/pnas.1313369110.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"128_CR39","doi-asserted-by":"publisher","first-page":"422","DOI":"10.1016\/S0006-3495(98)77529-0","volume":"75","author":"CN Pace","year":"1998","unstructured":"Pace CN, Scholtz JM: A helix propensity scale based on experimental studies of peptides and proteins. Biophys J. 1998, 75: 422-427. 10.1016\/S0006-3495(98)77529-0.","journal-title":"Biophys J"},{"key":"128_CR40","doi-asserted-by":"publisher","first-page":"13004","DOI":"10.1073\/pnas.0605420103","volume":"103","author":"A Belle","year":"2006","unstructured":"Belle A, Tanay A, Bitincka L, Shamir R, O\u2019Shea EK: Quantification of protein half-lives in the budding yeast proteome. Proc Natl Acad Sci U S A. 2006, 103: 13004-13009. 10.1073\/pnas.0605420103.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"128_CR41","doi-asserted-by":"publisher","first-page":"774","DOI":"10.1016\/j.cell.2009.07.038","volume":"138","author":"N Halabi","year":"2009","unstructured":"Halabi N, Rivoire O, Leibler S, Ranganathan R: Protein sectors: evolutionary units of three-dimensional structure. Cell. 2009, 138: 774-786. 10.1016\/j.cell.2009.07.038.","journal-title":"Cell"},{"key":"128_CR42","doi-asserted-by":"publisher","first-page":"1027","DOI":"10.1016\/j.bpj.2013.07.010","volume":"105","author":"JP Zayner","year":"2013","unstructured":"Zayner JP, Antoniou C, French AR, Hause RJ, Sosnick TR: Investigating models of protein function and allostery with a widespread mutational analysis of a light-activated protein. Biophys J. 2013, 105: 1027-1036. 10.1016\/j.bpj.2013.07.010.","journal-title":"Biophys J"},{"key":"128_CR43","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1186\/1754-1611-3-19","volume":"3","author":"D Chandran","year":"2009","unstructured":"Chandran D, Bergmann FT, Sauro HM: TinkerCell: modular CAD tool for synthetic biology. J Biol Eng. 2009, 3: 19-10.1186\/1754-1611-3-19.","journal-title":"J Biol Eng"},{"key":"128_CR44","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1038\/nbt1293","volume":"25","author":"T Drepper","year":"2007","unstructured":"Drepper T, Eggert T, Circolone F, Heck A, Krauss U, Guterl JK, Wendorff M, Losi A, Gartner W, Jaeger KE: Reporter proteins for in vivo fluorescence without oxygen. Nat Biotechnol. 2007, 25: 443-445. 10.1038\/nbt1293.","journal-title":"Nat Biotechnol"},{"key":"128_CR45","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/S0301-0104(03)00187-3","volume":"291","author":"SDM Islam","year":"2003","unstructured":"Islam SDM, Penzkofer A, Hegemann P: Quantum yield of triplet formation of riboflavin in aqueous solution and of flavin mononucleotide bound to the LOV1 domain of Phot1 from Chlamydomonas reinhardtii. Chem Phys. 2003, 291: 97-114. 10.1016\/S0301-0104(03)00187-3.","journal-title":"Chem Phys"},{"key":"128_CR46","doi-asserted-by":"publisher","first-page":"29345","DOI":"10.1074\/jbc.M113.506139","volume":"288","author":"T Gleichmann","year":"2013","unstructured":"Gleichmann T, Diensthuber RP, Moglich A: Charting the signal trajectory in a light-oxygen-voltage photoreceptor by random mutagenesis and covariance analysis. J Biol Chem. 2013, 288: 29345-29355. 10.1074\/jbc.M113.506139.","journal-title":"J Biol Chem"},{"key":"128_CR47","doi-asserted-by":"publisher","first-page":"17495","DOI":"10.1074\/jbc.M110.212027","volume":"286","author":"A Henderson","year":"2011","unstructured":"Henderson A, Erales J, Hoyt MA, Coffino P: Dependence of proteasome processing rate on substrate unfolding. J Biol Chem. 2011, 286: 17495-17502. 10.1074\/jbc.M110.212027.","journal-title":"J Biol Chem"},{"key":"128_CR48","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1126\/science.3018930","volume":"234","author":"A Bachmair","year":"1986","unstructured":"Bachmair A, Finley D, Varshavsky A: In vivo half-life of a protein is a function of its amino-terminal residue. Science. 1986, 234: 179-186. 10.1126\/science.3018930.","journal-title":"Science"},{"key":"128_CR49","doi-asserted-by":"publisher","first-page":"1273","DOI":"10.1126\/science.8122109","volume":"263","author":"RJ Dohmen","year":"1994","unstructured":"Dohmen RJ, Wu P, Varshavsky A: Heat-inducible degron: a method for constructing temperature-sensitive mutants. Science. 1994, 263: 1273-1276. 10.1126\/science.8122109.","journal-title":"Science"},{"key":"128_CR50","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1038\/nmeth.1401","volume":"6","author":"K Nishimura","year":"2009","unstructured":"Nishimura K, Fukagawa T, Takisawa H, Kakimoto T, Kanemaki M: An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nat Methods. 2009, 6: 917-922. 10.1038\/nmeth.1401.","journal-title":"Nat Methods"},{"key":"128_CR51","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1104\/pp.112.202184","volume":"160","author":"KA Havens","year":"2012","unstructured":"Havens KA, Guseman JM, Jang SS, Pierre-Jerome E, Bolten N, Klavins E, Nemhauser JL: A synthetic approach reveals extensive tunability of auxin signaling. Plant Physiol. 2012, 160: 135-142. 10.1104\/pp.112.202184.","journal-title":"Plant Physiol"},{"key":"128_CR52","doi-asserted-by":"publisher","first-page":"E3350","DOI":"10.1073\/pnas.1216880109","volume":"109","author":"AJ Holland","year":"2012","unstructured":"Holland AJ, Fachinetti D, Han JS, Cleveland DW: Inducible, reversible system for the rapid and complete degradation of proteins in mammalian cells. Proc Natl Acad Sci U S A. 2012, 109: E3350-E3357. 10.1073\/pnas.1216880109.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"128_CR53","doi-asserted-by":"publisher","first-page":"6359","DOI":"10.1093\/emboj\/20.22.6359","volume":"20","author":"G Pereira","year":"2001","unstructured":"Pereira G, Tanaka TU, Nasmyth K, Schiebel E: Modes of spindle pole body inheritance and segregation of the Bfa1p-Bub2p checkpoint protein complex. EMBO J. 2001, 20: 6359-6370. 10.1093\/emboj\/20.22.6359.","journal-title":"EMBO J"},{"key":"128_CR54","doi-asserted-by":"publisher","first-page":"3657","DOI":"10.1093\/emboj\/19.14.3657","volume":"19","author":"M Knop","year":"2000","unstructured":"Knop M, Strasser K: Role of the spindle pole body of yeast in mediating assembly of the prospore membrane during meiosis. EMBO J. 2000, 19: 3657-3667. 10.1093\/emboj\/19.14.3657.","journal-title":"EMBO J"},{"key":"128_CR55","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/S0076-6879(02)50954-X","volume":"350","author":"F Sherman","year":"2002","unstructured":"Sherman F: Getting started with yeast. Methods Enzymol. 2002, 350: 3-41. 10.1016\/S0076-6879(02)50954-X.","journal-title":"Methods Enzymol"},{"key":"128_CR56","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1007\/BF00340712","volume":"16","author":"RH Schiestl","year":"1989","unstructured":"Schiestl RH, Gietz RD: High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier. Curr Genet. 1989, 16: 339-346. 10.1007\/BF00340712.","journal-title":"Curr Genet"},{"key":"128_CR57","unstructured":"Current Protocols in Molecular Biology. 1995, John Wiley and Sons, New York, USA"},{"key":"128_CR58","doi-asserted-by":"publisher","first-page":"e67915","DOI":"10.1371\/journal.pone.0067915","volume":"8","author":"C Renicke","year":"2013","unstructured":"Renicke C, Spadaccini R, Taxis C: A tobacco etch virus protease with increased substrate tolerance at the P1\u2019 position. PLoS One. 2013, 8: e67915-10.1371\/journal.pone.0067915.","journal-title":"PLoS One"},{"key":"128_CR59","doi-asserted-by":"publisher","first-page":"25","DOI":"10.2144\/000112517","volume":"43","author":"TJ Collins","year":"2007","unstructured":"Collins TJ: ImageJ for microscopy. Biotechniques. 2007, 43: 25-30. 10.2144\/000112517.","journal-title":"Biotechniques"},{"key":"128_CR60","doi-asserted-by":"publisher","first-page":"4876","DOI":"10.1093\/nar\/25.24.4876","volume":"25","author":"JD Thompson","year":"1997","unstructured":"Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG: The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997, 25: 4876-4882. 10.1093\/nar\/25.24.4876.","journal-title":"Nucleic Acids Res"},{"key":"128_CR61","volume-title":"Randwertproblemmethoden zur Parameteridentifizierung in Systemen nichtlinearer Differentialgleichungen","author":"HG Bock","year":"1987","unstructured":"Bock HG: Randwertproblemmethoden zur Parameteridentifizierung in Systemen nichtlinearer Differentialgleichungen. 1987, Universit\u00e4t Bonn, Bonn"},{"key":"128_CR62","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1002\/gamm.200790024","volume":"30","author":"HG Bock","year":"2007","unstructured":"Bock HG, Kostina E, Schl\u00f4der JP: Numerical methods for parameter estimation in nonlinear differential algebraic equations. GAMM-Mitteilungen. 2007, 30: 376-408. 10.1002\/gamm.200790024.","journal-title":"GAMM-Mitteilungen"}],"container-title":["BMC Systems Biology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s12918-014-0128-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12918-014-0128-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12918-014-0128-9","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12918-014-0128-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,9,2]],"date-time":"2021-09-02T06:28:53Z","timestamp":1630564133000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcsystbiol.biomedcentral.com\/articles\/10.1186\/s12918-014-0128-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11,18]]},"references-count":62,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,12]]}},"alternative-id":["128"],"URL":"https:\/\/doi.org\/10.1186\/s12918-014-0128-9","relation":{},"ISSN":["1752-0509"],"issn-type":[{"value":"1752-0509","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,11,18]]},"assertion":[{"value":"22 July 2014","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 October 2014","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 November 2014","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"128"}}