{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T18:09:06Z","timestamp":1765303746252},"reference-count":14,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2015,6,4]],"date-time":"2015-06-04T00:00:00Z","timestamp":1433376000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Source Code Biol Med"],"published-print":{"date-parts":[[2015,12]]},"DOI":"10.1186\/s13029-015-0038-2","type":"journal-article","created":{"date-parts":[[2015,6,3]],"date-time":"2015-06-03T06:50:19Z","timestamp":1433314219000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["PrimerView: high-throughput primer design and visualization"],"prefix":"10.1186","volume":"10","author":[{"given":"Damien M.","family":"O\u2019Halloran","sequence":"first","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2015,6,4]]},"reference":[{"issue":"7492","key":"38_CR1","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1038\/507294a","volume":"507","author":"EC Hayden","year":"2014","unstructured":"Hayden EC. The $1,000 genome. Nature. 2014;507(7492):294\u20135.","journal-title":"Nature"},{"issue":"Web Server issu","key":"38_CR2","doi-asserted-by":"publisher","first-page":"W95","DOI":"10.1093\/nar\/gkp377","volume":"37","author":"B Contreras-Moreira","year":"2009","unstructured":"Contreras-Moreira B, Sachman-Ruiz B, Figueroa-Palacios I, Vinuesa P. primers4clades: a web server that uses phylogenetic trees to design lineage-specific PCR primers for metagenomic and diversity studies. Nucleic Acids Res. 2009;37(Web Server issue):W95\u2013100.","journal-title":"Nucleic Acids Res"},{"issue":"10","key":"38_CR3","doi-asserted-by":"publisher","first-page":"1611","DOI":"10.1101\/gr.361602","volume":"12","author":"JE Stajich","year":"2002","unstructured":"Stajich JE, Block D, Boulez K, Brenner SE, Chervitz SA, Dagdigian C, et al. The Bioperl toolkit: Perl modules for the life sciences. Genome Res. 2002;12(10):1611\u20138.","journal-title":"Genome Res"},{"issue":"5","key":"38_CR4","doi-asserted-by":"publisher","first-page":"1792","DOI":"10.1093\/nar\/gkh340","volume":"32","author":"RC Edgar","year":"2004","unstructured":"Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 2004;32(5):1792\u20137.","journal-title":"Nucleic Acids Res"},{"issue":"22","key":"38_CR5","doi-asserted-by":"publisher","first-page":"4673","DOI":"10.1093\/nar\/22.22.4673","volume":"22","author":"JD Thompson","year":"1994","unstructured":"Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994;22(22):4673\u201380.","journal-title":"Nucleic Acids Res"},{"issue":"4693","key":"38_CR6","doi-asserted-by":"publisher","first-page":"1435","DOI":"10.1126\/science.2983426","volume":"227","author":"DJ Lipman","year":"1985","unstructured":"Lipman DJ, Pearson WR. Rapid and sensitive protein similarity searches. Science. 1985;227(4693):1435\u201341.","journal-title":"Science"},{"key":"38_CR7","first-page":"191-2105-9-191","volume":"9","author":"K Li","year":"2008","unstructured":"Li K, Brownley A, Stockwell TB, Beeson K, McIntosh TC, Busam D, et al. Novel computational methods for increasing PCR primer design effectiveness in directed sequencing. BMC Bioinformatics. 2008;9:191-2105-9-191.","journal-title":"BMC Bioinformatics"},{"issue":"21","key":"38_CR8","doi-asserted-by":"publisher","first-page":"6409","DOI":"10.1093\/nar\/18.21.6409","volume":"18","author":"W Rychlik","year":"1990","unstructured":"Rychlik W, Spencer WJ, Rhoads RE. Optimization of the annealing temperature for DNA amplification in vitro. Nucleic Acids Res. 1990;18(21):6409\u201312.","journal-title":"Nucleic Acids Res"},{"issue":"6","key":"38_CR9","doi-asserted-by":"crossref","first-page":"325","DOI":"10.2144\/000114301","volume":"58","author":"DM O\u2019Halloran","year":"2015","unstructured":"O\u2019Halloran DM. STITCHER: a web resource for high-throughput design of primers for overlapping PCR applications. BioTechniques. 2015;58(6):325\u20138.","journal-title":"BioTechniques"},{"issue":"15","key":"38_CR10","doi-asserted-by":"publisher","first-page":"e115","DOI":"10.1093\/nar\/gks596","volume":"40","author":"A Untergasser","year":"2012","unstructured":"Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth BC, Remm M, et al. Primer3\u2013new capabilities and interfaces. Nucleic Acids Res. 2012;40(15):e115.","journal-title":"Nucleic Acids Res"},{"issue":"2","key":"38_CR11","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1093\/bioinformatics\/btn614","volume":"25","author":"W Qu","year":"2009","unstructured":"Qu W, Shen Z, Zhao D, Yang Y, Zhang C. MFEprimer: multiple factor evaluation of the specificity of PCR primers. Bioinformatics. 2009;25(2):276\u20138.","journal-title":"Bioinformatics"},{"issue":"Web Server issu","key":"38_CR12","doi-asserted-by":"publisher","first-page":"W205","DOI":"10.1093\/nar\/gks552","volume":"40","author":"W Qu","year":"2012","unstructured":"Qu W, Zhou Y, Zhang Y, Lu Y, Wang X, Zhao D, et al. MFEprimer-2.0: a fast thermodynamics-based program for checking PCR primer specificity. Nucleic Acids Res. 2012;40(Web Server issue):W205\u20138.","journal-title":"Nucleic Acids Res"},{"key":"38_CR13","doi-asserted-by":"publisher","first-page":"253-2105-9-253","DOI":"10.1186\/1471-2105-9-253","volume":"9","author":"FM You","year":"2008","unstructured":"You FM, Huo N, Gu YQ, Luo MC, Ma Y, Hane D, et al. BatchPrimer3: a high throughput web application for PCR and sequencing primer design. BMC Bioinformatics. 2008;9:253-2105-9-253.","journal-title":"BMC Bioinformatics"},{"key":"38_CR14","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1007\/978-1-59745-528-2_21","volume":"402","author":"O Marshall","year":"2007","unstructured":"Marshall O. Graphical design of primers with PerlPrimer. Methods Mol Biol. 2007;402:403\u201314.","journal-title":"Methods Mol Biol"}],"container-title":["Source Code for Biology and Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13029-015-0038-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s13029-015-0038-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13029-015-0038-2","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s13029-015-0038-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,10]],"date-time":"2022-05-10T19:14:30Z","timestamp":1652210070000},"score":1,"resource":{"primary":{"URL":"https:\/\/scfbm.biomedcentral.com\/articles\/10.1186\/s13029-015-0038-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6,4]]},"references-count":14,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["38"],"URL":"https:\/\/doi.org\/10.1186\/s13029-015-0038-2","relation":{},"ISSN":["1751-0473"],"issn-type":[{"value":"1751-0473","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,6,4]]},"assertion":[{"value":"7 April 2015","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 May 2015","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 June 2015","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"8"}}