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The 52-kDa Ro\/SSA antigen (Ro52) ribonucleoprotein represents an antigenic target strongly associated with the autoimmune response in mothers whose children have neonatal lupus and cardiac conduction disturbances, mainly congenital heart block. The objective of this study was to identify putative Ro52\/60-kDa Ro\/SSA antigen (Ro60) epitopes associated with neonatal lupus and congenital heart block. The reactivity of IgG antibodies present in the sera from mothers with systemic lupus erythematosus and Sj\u00f6gren's syndrome and in the sera from asymptomatic mothers (a longitudinal study of 192 samples from 66 subjects) was investigated by ELISA using Ro52, Ro60 and 48-kDa La\/SSB antigen proteins, as well as 45 synthetic peptides, 13\u201324 residues long, of Ro52\/Ro60 proteins. One to 19 samples collected before, during and after pregnancy were available for each mother. Forty-three disease controls selected randomly and normal sera were tested in parallel. Although no differences were found between Sj\u00f6gren's syndrome and asymptomatic mothers of group I, who had at least one infant with neonatal lupus, and of group II, who had healthy babies only, significant differences were observed between lupus mothers from both groups. In the former group of lupus mothers, a significantly higher frequency of antibodies to Ro52 peptides 107\u2013122 and 277\u2013292 was observed. Between 18 and 30 weeks of gestation, the period of risk, there was clearly an elevated level of antibodies reacting with Ro52 peptides 1\u201313, 277\u2013292 and 365\u2013382. Antibodies to Ro52 peptide 365\u2013382 have been shown previously to cross-react with residues 165\u2013185 of the heart 5-HT<jats:sub>4<\/jats:sub> serotoninergic receptor, and might be pathologically important. The level of these Ro52 antibody subsets decreased at the end of pregnancy and after delivery. IgG antibodies to Ro52 peptides 1\u201313, 107\u2013122, 277\u2013292 and 365\u2013382 may therefore represent important biomarkers to predict a complication in pregnant lupus women with Ro52 antibodies.<\/jats:p>","DOI":"10.1186\/ar1848","type":"journal-article","created":{"date-parts":[[2005,11,4]],"date-time":"2005-11-04T19:14:05Z","timestamp":1131131645000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["52-kDa Ro\/SSA epitopes preferentially recognized by antibodies from mothers of children with neonatal lupus and congenital heart block"],"prefix":"10.1186","volume":"8","author":[{"given":"Christine","family":"Fritsch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan","family":"Hoebeke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayet","family":"Dali","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Ricchiuti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A","family":"Isenberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Meyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylviane","family":"Muller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2005,11,4]]},"reference":[{"key":"1729_CR1","doi-asserted-by":"publisher","first-page":"1027","DOI":"10.1002\/ar.a.20072","volume":"280","author":"RM Clancy","year":"2004","unstructured":"Clancy RM, Buyon JP: Autoimmune-associated congenital heart block: dissecting the cascade from immunologic insult to relentless fibrosis. 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