{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T09:03:33Z","timestamp":1791277413337,"version":"4.1.0"},"reference-count":49,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2013,10,17]],"date-time":"2013-10-17T00:00:00Z","timestamp":1381968000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0\/"},{"start":{"date-parts":[[2013,10,17]],"date-time":"2013-10-17T00:00:00Z","timestamp":1381968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/2.0\/"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Arthritis Res Ther"],"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                        <jats:title>Introduction<\/jats:title>\n                        <jats:p>Extracellular ATP (eATP) is released by articular chondrocytes under physiological and pathological conditions. High eATP levels cause pathologic calcification, damage cartilage, and mediate pain. We recently showed that stable over-expression of the progressive ankylosis gene product, ANK, increased chondrocyte eATP levels, but the mechanisms of this effect remained unexplored. The purpose of this work was to further investigate mechanisms of eATP efflux in primary articular chondrocytes and to better define the role of ANK in this process.<\/jats:p>\n                     <\/jats:sec><jats:sec>\n                        <jats:title>Methods<\/jats:title>\n                        <jats:p>We measured eATP levels using a bioluminescence-based assay in adult porcine articular chondrocyte media with or without a 10 minute exposure to hypotonic stress. siRNAs for known ATP membrane transporters and pharmacologic inhibitors of ATP egress pathways were used to identify participants involved in chondrocyte eATP release.<\/jats:p>\n                     <\/jats:sec><jats:sec>\n                        <jats:title>Results<\/jats:title>\n                        <jats:p>eATP levels increased after exposure to hypotonic media in a calcium-dependent manner in monolayer and 3-dimensional agarose gel cultures (<jats:italic>p<\/jats:italic> &lt; 0.001). A potent transient receptor potential vanilloid 4 (TRPV4) agonist mimicked the effects of hypotonic media. ANK siRNA suppressed basal (<jats:italic>p<\/jats:italic> &lt; 0.01) and hypotonically-stressed (<jats:italic>p<\/jats:italic> &lt; 0.001) ATP levels. This effect was not mediated by altered extracellular pyrophosphate (ePPi) levels, and was mimicked by the ANK inhibitor, probenecid (<jats:italic>p<\/jats:italic> &lt; 0.001). The P2X7\/4 receptor inhibitor Brilliant Blue G also suppressed eATP efflux induced by hypotonic media (<jats:italic>p<\/jats:italic> &lt; 0.001), while ivermectin, a P2X4 receptor stimulant, increased eATP levels (<jats:italic>p<\/jats:italic> &lt; 0.001). Pharmacologic inhibitors of hemichannels, maxianion channels and other volume-sensitive eATP efflux pathways did not suppress eATP levels.<\/jats:p>\n                     <\/jats:sec><jats:sec>\n                        <jats:title>Conclusions<\/jats:title>\n                        <jats:p>These findings implicate ANK and P2X7\/4 receptors in chondrocyte eATP efflux. Understanding the mechanisms of eATP efflux may result in novel therapies for calcium crystal arthritis and osteoarthritis.<\/jats:p>\n                     <\/jats:sec>","DOI":"10.1186\/ar4337","type":"journal-article","created":{"date-parts":[[2013,10,17]],"date-time":"2013-10-17T13:02:22Z","timestamp":1382014942000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["The progressive ankylosis gene product ANK regulates extracellular ATP levels in primary articular chondrocytes"],"prefix":"10.1186","volume":"15","author":[{"given":"Ann K","family":"Rosenthal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia M","family":"Gohr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Mitton-Fitzgerald","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan K","family":"Lutz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George R","family":"Dubyak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lawrence M","family":"Ryan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2013,10,17]]},"reference":[{"key":"4416_CR1","first-page":"100","volume-title":"Nucleotides in the regulation of bone formation and resorption","author":"GR Dubyak","year":"2006","unstructured":"Dubyak GR: ATP release mechanisms. Nucleotides in the regulation of bone formation and resorption. Edited by: Burnstock G, Arnett T. 2006, London: CRC Press, 100-141."},{"key":"4416_CR2","first-page":"567","volume":"41","author":"SJ Millward-Sadler","year":"2004","unstructured":"Millward-Sadler SJ, Wright MO, Flatman PW, Salter DM: ATP in the mechanotransduction pathway of normal human chondrocytes. Biorheology. 2004, 41: 567-575.","journal-title":"Biorheology"},{"key":"4416_CR3","doi-asserted-by":"publisher","first-page":"1520","DOI":"10.1002\/art.1780351216","volume":"35","author":"LM Ryan","year":"1992","unstructured":"Ryan LM, Kurup IV, Derfus BA, Kushnaryov VM: ATP-induced chondrocalcinosis. Arthritis Rheum. 1992, 35: 1520-1524. 10.1002\/art.1780351216.","journal-title":"Arthritis Rheum"},{"key":"4416_CR4","doi-asserted-by":"publisher","first-page":"298","DOI":"10.1016\/0304-4165(94)90054-X","volume":"1201","author":"WS Leong","year":"1994","unstructured":"Leong WS, Russell RG, Caswell AM: Stimulation of cartilage resorption by extracellular ATP acting at P2-purinoceptors. Biochim Biophys Acta. 1994, 1201: 298-304. 10.1016\/0304-4165(94)90054-X.","journal-title":"Biochim Biophys Acta"},{"key":"4416_CR5","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1016\/S0165-1838(00)00137-5","volume":"81","author":"SG Hamilton","year":"2000","unstructured":"Hamilton SG, McMahon SB: ATP as a peripheral mediator of pain. J Autonomic Nervous System. 2000, 81: 187-194. 10.1016\/S0165-1838(00)00137-5.","journal-title":"J Autonomic Nervous System"},{"key":"4416_CR6","doi-asserted-by":"publisher","first-page":"14867","DOI":"10.1073\/pnas.1111101108","volume":"108","author":"C Jin","year":"2011","unstructured":"Jin C, Frayssinet P, Pelker R, Cwirka D, Hu B, Vignery A, Eisenbarth SC, Flavell RA: NLRP3 inflammasome plays a critical role in the pathogenesis of hydroxyapatite-associated arthropathy. PNAS USA. 2011, 108: 14867-14872. 10.1073\/pnas.1111101108.","journal-title":"PNAS USA"},{"key":"4416_CR7","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1097\/BOR.0b013e32830aba34","volume":"20","author":"CR Scanzello","year":"2008","unstructured":"Scanzello CR, Plass A, Crow MK: Innate immune system activation in osteoarthritis: is osteoarthritis a chronic wound?. Curr Opin Rheumatol. 2008, 20: 565-572. 10.1097\/BOR.0b013e32830aba34.","journal-title":"Curr Opin Rheumatol"},{"key":"4416_CR8","doi-asserted-by":"publisher","first-page":"139","DOI":"10.3109\/03008207.2010.491928","volume":"52","author":"JC Costello","year":"2011","unstructured":"Costello JC, Rosenthal AK, Kurup IV, Masuda I, Medhora M, Ryan LM: Parallel regulation of extracellular ATP and inorganic pyrophosphate: Roles of growth factors, transduction modulators and ANK. Conn Tissue Res. 2011, 52: 139-146. 10.3109\/03008207.2010.491928.","journal-title":"Conn Tissue Res"},{"key":"4416_CR9","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1126\/science.289.5477.265","volume":"289","author":"AM Ho","year":"2000","unstructured":"Ho AM, Johnson MD, Kingsley DM: Role of the mouse ank gene in tissue calcification and arthritis. Science. 2000, 289: 265-270. 10.1126\/science.289.5477.265.","journal-title":"Science"},{"key":"4416_CR10","doi-asserted-by":"publisher","first-page":"1017","DOI":"10.1086\/509881","volume":"79","author":"KA Gurley","year":"2006","unstructured":"Gurley KA, Reimer RJ, Kingsley DM: Biochemical and genetic analysis of ANK in arthritis and bone disease. Am J Human Genetics. 2006, 79: 1017-1029. 10.1086\/509881.","journal-title":"Am J Human Genetics"},{"key":"4416_CR11","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1002\/art.1780280409","volume":"28","author":"R Lm","year":"1985","unstructured":"Lm R, Wortmann RL, Karas B, McCarty DJ: Cartilage nucleoside triphosphate pyrophosphohydrolase. II. Role in extracellular pyrophosphate generation and nucleotide metabolism. Arthritis Rheum. 1985, 28: 413-418. 10.1002\/art.1780280409.","journal-title":"Arthritis Rheum"},{"key":"4416_CR12","first-page":"510","volume":"28","author":"M Garcia","year":"2010","unstructured":"Garcia M, Knight MM: Cyclic loading opens hemichannels to release ATP as part of a chondrocyte mechanotransduction pathway. J Orth Res. 2010, 28: 510-515.","journal-title":"J Orth Res"},{"key":"4416_CR13","doi-asserted-by":"publisher","first-page":"5683","DOI":"10.1073\/pnas.0800141105","volume":"105","author":"K Sawada","year":"2008","unstructured":"Sawada K, Echigo N, Juge N, Miyaji T, Otsuka M, Omote H, Yamamoto A, Moriyama Y: Identification of a vesicular nucleotide transporter. PNAS, USA. 2008, 105: 5683-5686. 10.1073\/pnas.0800141105.","journal-title":"PNAS, USA"},{"key":"4416_CR14","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1002\/jcp.21745","volume":"220","author":"IR Orriss","year":"2009","unstructured":"Orriss IR, Knight GE, Utting JC, Taylor SE, Burnstock G, Arnett TR: Hypoxia stimulates vesicular ATP release from rat osteoblasts. J Cell Physiol. 2009, 220: 155-162. 10.1002\/jcp.21745.","journal-title":"J Cell Physiol"},{"key":"4416_CR15","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1007\/s12576-008-0008-4","volume":"59","author":"RA Sabirov","year":"2009","unstructured":"Sabirov RA, Okada Y: The maxi-anion channel: a classical channel playing novel roles through an unidentified molecular entity. J Physiol Sci. 2009, 59: 3-21. 10.1007\/s12576-008-0008-4.","journal-title":"J Physiol Sci"},{"key":"4416_CR16","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1124\/jpet.108.146365","volume":"328","author":"W Ma","year":"2008","unstructured":"Ma W, Hui H, Pelegrin P, Suprenant A: Pharmacological characterization of pannexin-1 currents expressed in mammalian cells. J Pharmacol Exp Ther. 2008, 328: 409-418.","journal-title":"J Pharmacol Exp Ther"},{"key":"4416_CR17","doi-asserted-by":"publisher","first-page":"1378","DOI":"10.1523\/JNEUROSCI.3902-05.2006","volume":"26","author":"SO Suadicani","year":"2006","unstructured":"Suadicani SO, Brosnan CF, Scemes E: P2X7 receptors mediate ATP release and amplification of astrocytic intercellular Ca2+ signaling. J Neurosci. 2006, 26: 1378-1385. 10.1523\/JNEUROSCI.3902-05.2006.","journal-title":"J Neurosci"},{"key":"4416_CR18","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1096\/fj.09-138883","volume":"24","author":"SD Skaper","year":"2010","unstructured":"Skaper SD, Debetto P, Giusti P: The P2X7 purinergic receptor: from physiology to neurologic disorders. FASEB J. 2010, 24: 337-345. 10.1096\/fj.09-138883.","journal-title":"FASEB J"},{"key":"4416_CR19","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1038\/nm.2710","volume":"18","author":"RE Sorege","year":"2012","unstructured":"Sorege RE, Trang T, Dorman R, Smith SB, Beggs S, Ritchie J, Austin JS, Zaykin DV, Vander Meulen H, Costigan M, Herbert TA, Yarkoni-Abitbul M, Tichauer D, Livneh J, Gershon E, Zheng M, Tan K, John SL, Slade GD, Jordan J, Woolf CJ, Peltz G, Maixner W, Diatchenko L, Seltzer Z, Salter MW, Mogil JS: Genetically determined P2X7 receptor pore formation regulates variability in chronic pain sensitivity. Nature Med. 2012, 18: 595-599. 10.1038\/nm.2710.","journal-title":"Nature Med"},{"key":"4416_CR20","doi-asserted-by":"publisher","first-page":"13446","DOI":"10.1074\/jbc.M901255200","volume":"284","author":"M Boumechache","year":"2009","unstructured":"Boumechache M, Masin M, Edwardson JM, G\u00f3recki DC, Murrell-Lagnado R: Analysis of assembly and trafficking of native P2X4 and P2X7 receptor complexes in rodent immune cells. J Biol Chem. 2009, 284: 13446-13454. 10.1074\/jbc.M901255200.","journal-title":"J Biol Chem"},{"key":"4416_CR21","doi-asserted-by":"publisher","first-page":"C718","DOI":"10.1152\/ajpcell.00127.2005","volume":"291","author":"GH Chao","year":"2006","unstructured":"Chao GH, West AC, Hung CT: Chondrocyte intracellular calcium, cytoskeletal organization, and gene expression responses to dynamic osmotic loading. Am J Physiol Cell Physiol. 2006, 291: C718-C725. 10.1152\/ajpcell.00127.2005.","journal-title":"Am J Physiol Cell Physiol"},{"key":"4416_CR22","doi-asserted-by":"publisher","first-page":"2973","DOI":"10.1002\/art.27624","volume":"62","author":"AL Clark","year":"2010","unstructured":"Clark AL, Votta BJ, Kumar S, Leidtke W, Guilak F: Chondroprotective role of the osmotically sensitive ion channel transient receptor potential vanilloid 4. Arthritis Rheum. 2010, 62: 2973-2983. 10.1002\/art.27624.","journal-title":"Arthritis Rheum"},{"key":"4416_CR23","doi-asserted-by":"publisher","first-page":"1571","DOI":"10.1002\/1529-0131(200007)43:7<1571::AID-ANR22>3.0.CO;2-L","volume":"43","author":"RD Graff","year":"2000","unstructured":"Graff RD, Lazarowksi ER, Banes AJ, Lee GM: ATP release by mechanically loaded porcine chondrons in pellet culture. Arthritis Rheum. 2000, 43: 1571-1579. 10.1002\/1529-0131(200007)43:7<1571::AID-ANR22>3.0.CO;2-L.","journal-title":"Arthritis Rheum"},{"key":"4416_CR24","doi-asserted-by":"publisher","first-page":"1181","DOI":"10.1189\/jlb.0410211","volume":"88","author":"T Woehrle","year":"2010","unstructured":"Woehrle T, Yip L, Manohar M, Sumi Y, Yao Y, Chen Y, Junger WC: Hypertonic stress regulatesT cell function via pannexin-1 hemichannels and P2X receptors. J Leukoc Biol. 2010, 88: 1181-1188. 10.1189\/jlb.0410211.","journal-title":"J Leukoc Biol"},{"key":"4416_CR25","first-page":"125","volume":"7","author":"RK Korhonen","year":"2010","unstructured":"Korhonen RK, Han SK, Herzog W: Osmotic loading of in situ chondrocytes in their native environment. Mol Cell Biomechan. 2010, 7: 125-134.","journal-title":"Mol Cell Biomechan"},{"key":"4416_CR26","doi-asserted-by":"publisher","first-page":"808","DOI":"10.1038\/260808a0","volume":"260","author":"A Maroudas","year":"1976","unstructured":"Maroudas A: Balance between swelling pressure and collagen tension in normal and degenerate cartilage. Nature. 1976, 260: 808-809. 10.1038\/260808a0.","journal-title":"Nature"},{"key":"4416_CR27","doi-asserted-by":"publisher","first-page":"262","DOI":"10.1002\/jcp.1041540208","volume":"154","author":"JP Urban","year":"1993","unstructured":"Urban JP, Hall AC, Gehl KA: Regulation of matrix synthesis rates by the ionic and osmotic environment of articular chondrocytes. J Cell Physiol. 1993, 154: 262-270. 10.1002\/jcp.1041540208.","journal-title":"J Cell Physiol"},{"key":"4416_CR28","doi-asserted-by":"publisher","first-page":"904","DOI":"10.1002\/art.1780340717","volume":"34","author":"AK Rosenthal","year":"1991","unstructured":"Rosenthal AK, Cheung HS, Ryan LM: Transforming growth factor beta 1 stimulates inorganic pyrophosphate elaboration by porcine cartilage. Arthritis Rheum. 1991, 34: 904-911. 10.1002\/art.1780340717.","journal-title":"Arthritis Rheum"},{"key":"4416_CR29","doi-asserted-by":"publisher","first-page":"26277","DOI":"10.1074\/jbc.M111.260562","volume":"286","author":"L Seminario-Vidal","year":"2011","unstructured":"Seminario-Vidal L, Okada SF, Sesma JI, Kreda SM, van Heusden CA, Zhu Y, Jones LC, O'Neal WK, Penuela S, Laird DW, Boucher RC, Lazarowski ER: Rho signaling regulates pannexin 1-mediated ATP release from airway epithelia. J Biol Chem. 2011, 286: 26277-26286. 10.1074\/jbc.M111.260562.","journal-title":"J Biol Chem"},{"key":"4416_CR30","doi-asserted-by":"publisher","first-page":"C1308","DOI":"10.1152\/ajpcell.00333.2010","volume":"299","author":"A Li","year":"2010","unstructured":"Li A, Leung CT, Peterson-Yantorno K, Mitchell CH, Civan MM: Pathways for ATP release by bovine ciliary epithelial cells, the initial stop in purinergic regulation of aqueous humor inflow. Am J Physiol Cell Physiol. 2010, 299: C1308-C1317. 10.1152\/ajpcell.00333.2010.","journal-title":"Am J Physiol Cell Physiol"},{"key":"4416_CR31","first-page":"155","volume":"14","author":"CL Arkesteijn","year":"1976","unstructured":"Arkesteijn CL: A kinetic method for serum 5' -nucleotidase using stabilized glutamate dehydrogenase. J Clin Chem Clin Biochem. 1976, 14: 155-159.","journal-title":"J Clin Chem Clin Biochem"},{"key":"4416_CR32","doi-asserted-by":"publisher","first-page":"23331","DOI":"10.1074\/jbc.M302680200","volume":"278","author":"SM Joseph","year":"2003","unstructured":"Joseph SM, Buchakjian MR, Dubyak GR: Colocalization of ATP release sites and ecto-ATPase activity at the extracellular surface of human astrocytes. J Biol Chem. 2003, 278: 23331-23342. 10.1074\/jbc.M302680200.","journal-title":"J Biol Chem"},{"key":"4416_CR33","doi-asserted-by":"publisher","first-page":"C386","DOI":"10.1152\/ajpcell.00430.2009","volume":"298","author":"AE Blum","year":"2010","unstructured":"Blum AE, Walsh BC, Dubyak GR: Extracellular osmolarity modulates G protein-coupled receptor-dependent ATP release from 1321N1 astrocytoma cells. Am J Physiol Cell Physiol. 2010, 298: C386-C396. 10.1152\/ajpcell.00430.2009.","journal-title":"Am J Physiol Cell Physiol"},{"key":"4416_CR34","doi-asserted-by":"publisher","first-page":"C1751","DOI":"10.1152\/ajpcell.00010.2012","volume":"302","author":"M Shahidullah","year":"2012","unstructured":"Shahidullah M, Mandal A, Delamere NA: TRPV4 in porcine lens epithelium regulates hemichannel-mediated ATP release and Na-K-ATPase activity. Am J Physiol Cell Physiol. 2012, 302: C1751-C1761. 10.1152\/ajpcell.00010.2012.","journal-title":"Am J Physiol Cell Physiol"},{"key":"4416_CR35","first-page":"896","volume":"21","author":"AK Rosenthal","year":"1993","unstructured":"Rosenthal AK, Ryan LM: Probenecid inhibits transforming growth factor \u03b21 induced pyrophosphate elaboration by chondrocytes. J Rheumatol. 1993, 21: 896-900.","journal-title":"J Rheumatol"},{"key":"4416_CR36","doi-asserted-by":"publisher","first-page":"825071","DOI":"10.1038\/sj.emboj.7601378","volume":"25","author":"P Pelegrin","year":"2006","unstructured":"Pelegrin P, Surprenant A: Pannexin-1 mediates large pore formation and interleukin-1\u03b2 release by the ATP-gated P2X7 receptor. EMBO J. 2006, 25: 825071-","journal-title":"EMBO J"},{"key":"4416_CR37","doi-asserted-by":"publisher","first-page":"728","DOI":"10.1002\/glia.22301","volume":"60","author":"LP Bernier","year":"2012","unstructured":"Bernier LP, Ase AR, Bou\u00e9-Grabot E, S\u00e9gu\u00e9la P: P2X4 receptor channels form large noncytolytic pores in resting and activated microglia. Glia. 2012, 60: 728-737. 10.1002\/glia.22301.","journal-title":"Glia"},{"key":"4416_CR38","doi-asserted-by":"publisher","first-page":"1447","DOI":"10.1124\/mol.107.035980","volume":"72","author":"C Guo","year":"2007","unstructured":"Guo C, Masin M, Qureshi OS, Murrell-Lagnado RD: Evidence for functional P2X4\/P2X7 heteromeric receptors. Mol Pharmacol. 2007, 72: 1447-1456. 10.1124\/mol.107.035980.","journal-title":"Mol Pharmacol"},{"key":"4416_CR39","doi-asserted-by":"publisher","first-page":"13680","DOI":"10.1074\/jbc.M211570200","volume":"278","author":"F Berenbaum","year":"2003","unstructured":"Berenbaum F, Humbert L, Bereziat G, Thirion S: Concomittant recruitment of ERK1\/2 and p38 MAPK signalling pathway is required for activation of cytoplasmic phospholipase A2 via ATP in articular chondrocytes. J Biol Chem. 2003, 278: 13680-13687. 10.1074\/jbc.M211570200.","journal-title":"J Biol Chem"},{"key":"4416_CR40","doi-asserted-by":"publisher","first-page":"3471","DOI":"10.1113\/jphysiol.2011.207829","volume":"589","author":"H Mihara","year":"2011","unstructured":"Mihara H, Boudaka A, Sugiyama A, Moriyama Y, Tominaga M: Transient receptor potential vallinoid 4 (TRPV4)-dependent calcium influx and ATP release in mouse oesophageal keratinocytes. J Physiol. 2011, 589: 3471-3482. 10.1113\/jphysiol.2011.207829.","journal-title":"J Physiol"},{"key":"4416_CR41","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1016\/j.joca.2003.12.004","volume":"12","author":"K Johnson","year":"2004","unstructured":"Johnson K, Terkeltaub R: Upregulated ank expression in osteoarthritis can promote both chondrocyte MMP-13 expression and calcification via chondrocyte extracellular PPi excess. Osteoarthritis Cart. 2004, 12: 321-335. 10.1016\/j.joca.2003.12.004.","journal-title":"Osteoarthritis Cart"},{"key":"4416_CR42","doi-asserted-by":"publisher","first-page":"3218","DOI":"10.1002\/art.10632","volume":"46","author":"J Hirose","year":"2002","unstructured":"Hirose J, Ryan LM, Masuda I: Up-regulated expression of cartilage intermediate-layer protein and ANK in patients with calcium pyrophosphate dihydrate crystal deposition disease. Arthritis Rheum. 2002, 46: 3218-3229. 10.1002\/art.10632.","journal-title":"Arthritis Rheum"},{"key":"4416_CR43","doi-asserted-by":"publisher","first-page":"10572","DOI":"10.1074\/jbc.M109.050534","volume":"285","author":"F Cailotto","year":"2010","unstructured":"Cailotto F, Sebillaud S, Netter P, Jouzeau JY, Bianchi A: The inorganic pyrophosphate transporter ANK preserves the differentiated phenotype of articular chondrocyte. J Biol Chem. 2010, 285: 10572-10582. 10.1074\/jbc.M109.050534.","journal-title":"J Biol Chem"},{"key":"4416_CR44","first-page":"831","volume":"4","author":"HG Xu","year":"2011","unstructured":"Xu HG, Hu CJ, Wang H, Liu P, Yang XM, Zhang Y, Want LT: Effects of mechanical strain on ANK, ENPP1 and TGF-B1 expression in rat endplate chondrocytes in vitro. Mol Med Reports. 2011, 4: 831-835.","journal-title":"Mol Med Reports"},{"key":"4416_CR45","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/bdd.693","volume":"31","author":"AL VanWert","year":"2010","unstructured":"VanWert AL, Gionfriddo MR, Sweet DH: Organic Anion Transporters: Discovery, Pharmacology, Regulation and Roles in Pathophysiology. Biopharm Drug Dispos. 2010, 31: 1-71.","journal-title":"Biopharm Drug Dispos"},{"key":"4416_CR46","doi-asserted-by":"publisher","first-page":"135","DOI":"10.2165\/00003088-198106020-00004","volume":"6","author":"RF Cunningham","year":"1981","unstructured":"Cunningham RF, Israili ZH, Dayton PG: Clinical pharmacokinetics of probenecid. Clin Pharmokinetics. 1981, 6: 135-151. 10.2165\/00003088-198106020-00004.","journal-title":"Clin Pharmokinetics"},{"key":"4416_CR47","doi-asserted-by":"publisher","first-page":"6553","DOI":"10.4049\/jimmunol.1100478","volume":"186","author":"Y Qu","year":"2011","unstructured":"Qu Y, Misaghi S, Newton K, Gilmour LL, Louie S, Cupp JE, Dubyak GR, Hackos D, Dixit VM: Pannexin-1 is required for ATP release during apoptosis but not for inflammasome activation. J Immunol. 2011, 186: 6553-6561. 10.4049\/jimmunol.1100478.","journal-title":"J Immunol"},{"key":"4416_CR48","doi-asserted-by":"publisher","first-page":"18948","DOI":"10.1074\/jbc.M110.127027","volume":"285","author":"T Iwamoto","year":"2010","unstructured":"Iwamoto T, Nakmura T, Doyle A, Ishikawa M, DeVega S, Fukumoto S, Yamada Y: Pannexin 3 regulates intracellular ATP\/cAMP levels and promotes chondrocyte differentiation. J Biol Chem. 2010, 285: 18948-18958. 10.1074\/jbc.M110.127027.","journal-title":"J Biol Chem"},{"key":"4416_CR49","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1002\/art.1780350314","volume":"35","author":"PG Mitchell","year":"1992","unstructured":"Mitchell PG, Struve JA, McCarthy GM, Cheung HS: Basic calcium phosphate crystals stimulate cell proliferation and collagenase message accumulation in cultured adult articular chondrocytes. Arthritis Rheum. 1992, 35: 343-350. 10.1002\/art.1780350314.","journal-title":"Arthritis Rheum"}],"container-title":["Arthritis Research &amp; Therapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/ar4337.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/ar4337\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/ar4337.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T10:57:33Z","timestamp":1728385053000},"score":1,"resource":{"primary":{"URL":"https:\/\/arthritis-research.biomedcentral.com\/articles\/10.1186\/ar4337"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10,17]]},"references-count":49,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2013,10]]}},"alternative-id":["4416"],"URL":"https:\/\/doi.org\/10.1186\/ar4337","relation":{},"ISSN":["1478-6354"],"issn-type":[{"value":"1478-6354","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,17]]},"assertion":[{"value":"28 March 2013","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 October 2013","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 October 2013","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"R154"}}