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The aim of this study was to investigate the protective effect of \u03b17 nicotinic acetylcholine receptor (\u03b17nAChR), and to determine the underlying mechanism of its activity.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>The expression and distribution of \u03b17nAChR in the intestinal tissue of patients with ulcerative colitis and Crohn\u2019s disease were analyzed. The effects of vagal excitation on murine experimental colitis were investigated. The colitis model was induced in C57BL\/6 mice by the administration of 3% dextran sulfate sodium (DSS). The therapeutic group received treatment with the \u03b17nAChR agonist PNU-282987 by intraperitoneal injection.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Our results showed that there was significantly increased expression of \u03b17nAChR in colitis and Crohn\u2019s disease intestinal tissue, and its expression was mainly located in macrophages and neutrophils, which were extensively infiltrated in the disease status. Treatment with an \u03b17nAChR agonist potently ameliorated the DSS-induced illness state, including weight loss, stool consistency, bleeding, colon shortening, and colon histological injury. \u03b17nAChR agonist exerted anti-inflammatory effects in DSS colitis mice by suppressing the secretion of multiple types of proinflammatory factors, such as IL6, TNF\u03b1, and IL1\u03b2, and it also inhibited the colonic infiltration of inflammatory cells by blocking the DSS-induced overactivation of the NF-\u03baB and MAPK signaling pathways. Mechanistically, activation of \u03b17nAChR decreased the number of infiltrated M1 macrophages in the colitis intestine and inhibited the phagocytosis ability of macrophages, which were activated in response to LPS stimulation.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Thus, an \u03b17nAChR agonist ameliorated colonic pathology and inflammation in DSS-induced colitis mice by blocking the activation of inflammatory M1 macrophages.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s10020-022-00532-2","type":"journal-article","created":{"date-parts":[[2022,9,4]],"date-time":"2022-09-04T04:02:41Z","timestamp":1662264161000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Protective effect of \u03b17 nicotinic acetylcholine receptor activation on experimental colitis and its mechanism"],"prefix":"10.1186","volume":"28","author":[{"given":"Wenyuan","family":"Pu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenzi","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junaid","family":"Wazir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lulu","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranran","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiyi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saifang","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoyi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2868-5112","authenticated-orcid":false,"given":"Hongwei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,9,4]]},"reference":[{"key":"532_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2022.104622","volume":"136","author":"N Alen","year":"2022","unstructured":"Alen N. 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