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However, there is uncertainty as to how sample handling practices affect performance of tests to detect RSV. The aim of this study was to determine whether RSV RNA remains reliably detectable in nasopharyngeal\/oropharyngeal (NP\/OP) samples, saliva, and sputum samples over time.<\/jats:p>\n                  <jats:p>Respiratory samples were collected as part of a prospective observational study of acute lower respiratory tract disease (aLRTD) hospitalisations in adults in Bristol (UK). Samples that tested positive by PCR on receipt (0\u00a0h), were re-tested at 24\u00a0h having been stored at room temperature. We found that all but one of the samples PCR-positive for RSV at 0\u00a0h remained positive at 24\u00a0h, across all sample types and RSV strains. Ct values for NP\/OP and saliva samples were significantly lower at 24\u00a0h than at 0\u00a0h, suggesting potential low-level viral replication in the samples. 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Informed consent was obtained from patients with capacity, and declarations for participation from consultees for individuals lacking capacity. If it was not practicable to approach individuals for consent, data were included using approval from the Clinical Advisory Group under Sect.\u00a0251 of the 2006 NHS Act.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"CH is Principal Investigator of the AvonCAP study which is an investigator-led University of Bristol study funded by Pfizer and has previously received support from the NIHR in an Academic Clinical Fellowship. JO is a Co-Investigator on the AvonCAP Study. LD is further supported by UKRI through the JUNIPER consortium (grant number MR\/V038613\/1), MRC (grant number MC\/PC\/19067), EPSRC (EP\/V051555\/1 and The Alan Turing Institute, grant EP\/N510129\/1). AF was a member of the Joint Committee on Vaccination and Immunization (JCVI) until January 2024 and remains a member of the JCVI RSV subcommittee. In addition to receiving funding from Pfizer as Chief Investigator of this study, he leads another project investigating transmission of respiratory bacteria in families jointly funded by Pfizer and the Gates Foundation and is an investigator in trials of COVID-19 vaccines including ChAdOx1nCOV-19, Janssen, Sanofi and Valneva vaccines. NM is supported by the National Institute for Health and Care Research Bristol Biomedical Research Centre. The views expressed are those of the author(s) and not necessarily those of the NIHR or the Department of Health and Social Care. All authors with Pfizer affiliations (EB, JS, ML, KS, BDG) were employees of Pfizer at the time of this work and may own Pfizer stock.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"342"}}