{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T11:28:13Z","timestamp":1793964493469,"version":"4.8.0"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T00:00:00Z","timestamp":1765584000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"},{"start":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T00:00:00Z","timestamp":1768867200000},"content-version":"vor","delay-in-days":38,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR202111140152, ZR2021MH293"],"award-info":[{"award-number":["ZR202111140152, ZR2021MH293"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell Mol Biol Lett"],"DOI":"10.1186\/s11658-025-00832-5","type":"journal-article","created":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T16:23:44Z","timestamp":1765643024000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["CDCP1 allosterically regulates the AMPK \u03b11 subunit to enhance fatty acid oxidation in osteoblasts"],"prefix":"10.1186","volume":"31","author":[{"given":"Xiang","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weichun","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinlong","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenqian","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Limin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangjun","family":"Jiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunzhen","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,12,13]]},"reference":[{"issue":"1","key":"832_CR1","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1038\/nrm3254","volume":"13","author":"F Long","year":"2011","unstructured":"Long F. Building strong bones: molecular regulation of the osteoblast lineage. Nat Rev Mol Cell Biol. 2011;13(1):27\u201338.","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"11","key":"832_CR2","doi-asserted-by":"publisher","first-page":"1979","DOI":"10.1128\/MCB.01343-14","volume":"35","author":"JL Frey","year":"2015","unstructured":"Frey JL, Li Z, Ellis JM, Zhang Q, Farber CR, Aja S, et al. Wnt-Lrp5 signaling regulates fatty acid metabolism in the osteoblast. Mol Cell Biol. 2015;35(11):1979\u201391.","journal-title":"Mol Cell Biol"},{"issue":"6","key":"832_CR3","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1007\/s002239900644","volume":"64","author":"KM Chiu","year":"1999","unstructured":"Chiu KM, Keller ET, Crenshaw TD, Gravenstein S. Carnitine and dehydroepiandrosterone sulfate induce protein synthesis in porcine primary osteoblast-like cells. Calcif Tissue Int. 1999;64(6):527\u201333.","journal-title":"Calcif Tissue Int"},{"issue":"2","key":"832_CR4","doi-asserted-by":"publisher","first-page":"130","DOI":"10.1007\/s11914-018-0427-y","volume":"16","author":"L Singh","year":"2018","unstructured":"Singh L, Tyagi S, Myers D, Duque G. Good, bad, or ugly: the biological roles of bone marrow fat. Curr Osteoporos Rep. 2018;16(2):130\u20137.","journal-title":"Curr Osteoporos Rep"},{"issue":"4","key":"832_CR5","doi-asserted-by":"publisher","first-page":"982","DOI":"10.1111\/j.1582-4934.2009.00751.x","volume":"14","author":"A Elbaz","year":"2010","unstructured":"Elbaz A, Wu X, Rivas D, Gimble JM, Duque G. Inhibition of fatty acid biosynthesis prevents adipocyte lipotoxicity on human osteoblasts in vitro. J Cell Mol Med. 2010;14(4):982\u201391.","journal-title":"J Cell Mol Med"},{"issue":"12","key":"832_CR6","doi-asserted-by":"publisher","first-page":"1382","DOI":"10.1242\/bio.20136700","volume":"2","author":"K Gunaratnam","year":"2013","unstructured":"Gunaratnam K, Vidal C, Boadle R, Thekkedam C, Duque G. Mechanisms of palmitate-induced cell death in human osteoblasts. Biol Open. 2013;2(12):1382\u20139.","journal-title":"Biol Open"},{"issue":"2","key":"832_CR7","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1016\/j.bone.2008.03.022","volume":"43","author":"A Niemeier","year":"2008","unstructured":"Niemeier A, Niedzielska D, Secer R, Schilling A, Merkel M, Enrich C, et al. Uptake of postprandial lipoproteins into bone in vivo: impact on osteoblast function. Bone. 2008;43(2):230\u20137.","journal-title":"Bone"},{"issue":"6","key":"832_CR8","doi-asserted-by":"publisher","first-page":"1092","DOI":"10.1016\/j.bone.2009.02.022","volume":"44","author":"JF Griffith","year":"2009","unstructured":"Griffith JF, Yeung DK, Ahuja AT, Choy CW, Mei WY, Lam SS, et al. A study of bone marrow and subcutaneous fatty acid composition in subjects of varying bone mineral density. Bone. 2009;44(6):1092\u20136.","journal-title":"Bone"},{"issue":"1","key":"832_CR9","doi-asserted-by":"publisher","first-page":"5","DOI":"10.18632\/oncoscience.286","volume":"3","author":"Y He","year":"2016","unstructured":"He Y, Harrington BS, Hooper JD. New crossroads for potential therapeutic intervention in cancer \u2013 intersections between CDCP1, EGFR family members and downstream signaling pathways. Oncoscience. 2016;3(1):5\u20138.","journal-title":"Oncoscience"},{"issue":"9","key":"832_CR10","doi-asserted-by":"publisher","first-page":"2259","DOI":"10.1158\/0008-5472.CAN-20-2978","volume":"81","author":"T Khan","year":"2021","unstructured":"Khan T, Kryza T, Lyons NJ, He Y, Hooper JD. The CDCP1 signaling hub: a target for cancer detection and therapeutic intervention. Cancer Res. 2021;81(9):2259\u201369.","journal-title":"Cancer Res"},{"issue":"32","key":"832_CR11","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1703791114","volume":"114","author":"HJ Wright","year":"2017","unstructured":"Wright HJ, Hou J, Xu B, Cortez M, Potma EO, Tromberg BJ, et al. CDCP1 drives triple-negative breast cancer metastasis through reduction of lipid-droplet abundance and stimulation of fatty acid oxidation. Proc Natl Acad Sci U S A. 2017;114(32):E6556-e65.","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"9","key":"832_CR12","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.1369","volume":"13","author":"L Zhang","year":"2023","unstructured":"Zhang L, Jiao G, You Y, Li X, Liu J, Sun Z, et al. Arginine methylation of PPP1CA by CARM1 regulates glucose metabolism and affects osteogenic differentiation and osteoclastic differentiation. Clin Transl Med. 2023;13(9):e1369.","journal-title":"Clin Transl Med"},{"issue":"1","key":"832_CR13","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1038\/s41467-023-35801-z","volume":"14","author":"J Li","year":"2023","unstructured":"Li J, Yao Z, Liu X, Duan R, Yi X, Ayoub A, et al. Tgf\u03b21(+)ccr5(+) neutrophil subset increases in bone marrow and causes age-related osteoporosis in male mice. Nat Commun. 2023;14(1):159.","journal-title":"Nat Commun"},{"key":"832_CR14","doi-asserted-by":"publisher","first-page":"12724","DOI":"10.1038\/srep12724","volume":"5","author":"J Li","year":"2015","unstructured":"Li J, He W, Liao B, Yang J. FFA-ROS-P53-mediated mitochondrial apoptosis contributes to reduction of osteoblastogenesis and bone mass in type 2 diabetes mellitus. Sci Rep. 2015;5:12724.","journal-title":"Sci Rep"},{"issue":"9","key":"832_CR15","doi-asserted-by":"publisher","first-page":"4771","DOI":"10.1021\/acs.jcim.1c00341","volume":"61","author":"Y Feng","year":"2021","unstructured":"Feng Y, Zhang K, Wu Q, Huang SY. Nldock: a fast nucleic acid-ligand docking algorithm for modeling RNA\/DNA-ligand complexes. J Chem Inf Model. 2021;61(9):4771\u201382.","journal-title":"J Chem Inf Model"},{"issue":"3","key":"832_CR16","doi-asserted-by":"publisher","first-page":"838","DOI":"10.1016\/j.drudis.2021.10.013","volume":"27","author":"Y Feng","year":"2022","unstructured":"Feng Y, Yan Y, He J, Tao H, Wu Q, Huang SY. Docking and scoring for nucleic acid-ligand interactions: principles and current status. Drug Discov Today. 2022;27(3):838\u201347.","journal-title":"Drug Discov Today"},{"issue":"1","key":"832_CR17","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1093\/gerona\/glz149","volume":"75","author":"A Al Saedi","year":"2020","unstructured":"Al Saedi A, C AG, D EM, Hayes A, Duque G. Rapamycin affects palmitate-induced lipotoxicity in osteoblasts by modulating apoptosis and autophagy. J Gerontol A Biol Sci Med Sci. 2020;75(1):58\u201363.","journal-title":"J Gerontol A Biol Sci Med Sci"},{"issue":"3","key":"832_CR18","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1016\/j.tcb.2015.10.013","volume":"26","author":"DG Hardie","year":"2016","unstructured":"Hardie DG, Schaffer BE, Brunet A. AMPK: an energy-sensing pathway with multiple inputs and outputs. Trends Cell Biol. 2016;26(3):190\u2013201.","journal-title":"Trends Cell Biol"},{"issue":"10","key":"832_CR19","doi-asserted-by":"publisher","first-page":"774","DOI":"10.1038\/nrm2249","volume":"8","author":"DG Hardie","year":"2007","unstructured":"Hardie DG. AMP-activated\/SNF1 protein kinases: conserved guardians of cellular energy. Nat Rev Mol Cell Biol. 2007;8(10):774\u201385.","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"6","key":"832_CR20","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1016\/j.molcel.2017.05.032","volume":"66","author":"D Garcia","year":"2017","unstructured":"Garcia D, Shaw RJ. AMPK: mechanisms of cellular energy sensing and restoration of metabolic balance. Mol Cell. 2017;66(6):789\u2013800.","journal-title":"Mol Cell"},{"issue":"44","key":"832_CR21","doi-asserted-by":"publisher","first-page":"27879","DOI":"10.1074\/jbc.271.44.27879","volume":"271","author":"SA Hawley","year":"1996","unstructured":"Hawley SA, Davison M, Woods A, Davies SP, Beri RK, Carling D, et al. Characterization of the AMP-activated protein kinase kinase from rat liver and identification of threonine 172 as the major site at which it phosphorylates AMP-activated protein kinase. J Biol Chem. 1996;271(44):27879\u201387.","journal-title":"J Biol Chem"},{"issue":"1","key":"832_CR22","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1074\/jbc.M605790200","volume":"282","author":"T Pang","year":"2007","unstructured":"Pang T, Xiong B, Li J-Y, Qiu B-Y, Jin G-Z, Shen J-K, et al. Conserved \u03b1-helix acts as autoinhibitory sequence in AMP-activated protein kinase \u03b1 subunits. J Biol Chem. 2007;282(1):495\u2013506.","journal-title":"J Biol Chem"},{"issue":"1","key":"832_CR23","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1042\/bj2480129","volume":"248","author":"G Adamek","year":"1987","unstructured":"Adamek G, Felix R, Guenther HL, Fleisch H. Fatty acid oxidation in bone tissue and bone cells in culture. Characterization and hormonal influences. Biochem J. 1987;248(1):129\u201337.","journal-title":"Biochem J"},{"issue":"1","key":"832_CR24","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1007\/s00198-016-3844-8","volume":"28","author":"T An","year":"2017","unstructured":"An T, Hao J, Sun S, Li R, Yang M, Cheng G, et al. Efficacy of statins for osteoporosis: a systematic review and meta-analysis. Osteoporos Int. 2017;28(1):47\u201357.","journal-title":"Osteoporos Int"},{"issue":"2","key":"832_CR25","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1007\/s11914-018-0426-z","volume":"16","author":"B Lecka-Czernik","year":"2018","unstructured":"Lecka-Czernik B, Baroi S, Stechschulte LA, Chougule AS. Marrow fat-a new target to treat bone diseases? Curr Osteoporos Rep. 2018;16(2):123\u20139.","journal-title":"Curr Osteoporos Rep"},{"issue":"4","key":"832_CR26","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1038\/s41580-022-00547-x","volume":"24","author":"GR Steinberg","year":"2023","unstructured":"Steinberg GR, Hardie DG. New insights into activation and function of the AMPK. Nat Rev Mol Cell Biol. 2023;24(4):255\u201372.","journal-title":"Nat Rev Mol Cell Biol"},{"issue":"16","key":"832_CR27","doi-asserted-by":"publisher","first-page":"2987","DOI":"10.1111\/febs.13698","volume":"283","author":"FA Ross","year":"2016","unstructured":"Ross FA, MacKintosh C, Hardie DG. AMP-activated protein kinase: a cellular energy sensor that comes in 12 flavours. FEBS J. 2016;283(16):2987\u20133001.","journal-title":"FEBS J"},{"issue":"7453","key":"832_CR28","doi-asserted-by":"publisher","DOI":"10.1038\/nature12189","volume":"498","author":"L Chen","year":"2013","unstructured":"Chen L, Xin FJ, Wang J, Hu J, Zhang YY, Wan S, et al. Conserved regulatory elements in AMPK. Nature. 2013;498(7453):E8-10.","journal-title":"Nature"},{"issue":"10","key":"832_CR29","doi-asserted-by":"publisher","first-page":"1237","DOI":"10.1038\/cr.2013.121","volume":"23","author":"FJ Xin","year":"2013","unstructured":"Xin FJ, Wang J, Zhao RQ, Wang ZX, Wu JW. Coordinated regulation of AMPK activity by multiple elements in the \u03b1-subunit. Cell Res. 2013;23(10):1237\u201340.","journal-title":"Cell Res"},{"issue":"1","key":"832_CR30","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1038\/cr.2014.150","volume":"25","author":"X Li","year":"2015","unstructured":"Li X, Wang L, Zhou XE, Ke J, de Waal PW, Gu X, et al. Structural basis of AMPK regulation by adenine nucleotides and glycogen. Cell Res. 2015;25(1):50\u201366.","journal-title":"Cell Res"}],"container-title":["Cellular & Molecular Biology Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s11658-025-00832-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s11658-025-00832-5","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s11658-025-00832-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T11:59:14Z","timestamp":1768910354000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1186\/s11658-025-00832-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,13]]},"references-count":30,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["832"],"URL":"https:\/\/doi.org\/10.1186\/s11658-025-00832-5","relation":{},"ISSN":["1689-1392"],"issn-type":[{"value":"1689-1392","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,13]]},"assertion":[{"value":"4 June 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 November 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 December 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The experimental plan received approval from the Medical Ethics Committee of Shandong University Qilu Hospital (no. KYLL-202306-049; date of approval: 2023-07-06), which operates in accordance with the ethical guidelines of the International Council for Laboratory Animal Science.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"7"}}