{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,4]],"date-time":"2024-05-04T00:36:11Z","timestamp":1714782971659},"reference-count":45,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T00:00:00Z","timestamp":1714694400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T00:00:00Z","timestamp":1714694400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Genes Nutr"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Evidences have shown that obesity is influenced by various factors, including various hormones such as thyroid hormones and the body\u2019s metabolism rate. It seems that practical solutions such as weight loss diets and common drugs can affect these potential disorders. In this study, we investigate one of these common drugs, N-Acetylcysteine (NAC), on expressions of <jats:italic>UCP1<\/jats:italic> and factors related to thyroid function in adults with obesity.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods and analysis<\/jats:title>\n                <jats:p>The current investigation was carried out as a randomized clinical trial (RCT) including 43 adults with obesity who were potential candidates for bariatric surgery. These individuals were randomly divided into two groups: 600\u00a0mg of NAC (<jats:italic>n<\/jats:italic>\u2009=\u200922) or placebo (<jats:italic>n<\/jats:italic>\u2009=\u200921) for a duration of 8 weeks. Visceral adipose tissue was utilized in the context of bariatric surgery to investigate the gene expression of <jats:italic>UCP1<\/jats:italic> and thyroid function. Polymerase chain reaction (PCR) was performed in duplicate for <jats:italic>UCP1, DIO2, DIO3, THR\u03b1<\/jats:italic> and <jats:italic>\u03b2<\/jats:italic>, and <jats:italic>18s<\/jats:italic> RNA (as an internal control) using the provided instructions to investigate the expression of the respective genes.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Our findings revealed that after 8 weeks compared to placebo, NAC caused a significant decrease in the expression of the <jats:italic>DIO3<\/jats:italic> gene as one of the genes related to thyroid function and metabolism. However, regarding other related genes, no statistically significant was found (despite the increase in <jats:italic>UCP1, DIO2<\/jats:italic>, and <jats:italic>THR\u03b1<\/jats:italic> expression and decrease in <jats:italic>THR\u03b2<\/jats:italic> expression). In addition, after adjustment of possible confounders, no significant effect was observed on anthropometric factors and serum levels of thyroid hormones.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>The results of this study indicate that, following an 8-week period, NAC effectively decreases the expression of the <jats:italic>DIO3<\/jats:italic> gene in the visceral fat tissue, in comparison to the placebo.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12263-024-00744-7","type":"journal-article","created":{"date-parts":[[2024,5,3]],"date-time":"2024-05-03T14:02:21Z","timestamp":1714744941000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of N-acetylcysteine on the expressions of UCP1 and factors related to thyroid function in visceral adipose tissue of obese adults: a randomized, double-blind clinical trial"],"prefix":"10.1186","volume":"19","author":[{"given":"Mohammad Hassan","family":"Sohouli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ghazaleh","family":"Eslamian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyed Hossein","family":"Ardehali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyed Ahmad","family":"Raeissadat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ghazaleh","family":"Shimi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katayoun","family":"Pourvali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hamid","family":"Zand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,5,3]]},"reference":[{"issue":"5","key":"744_CR1","doi-asserted-by":"publisher","first-page":"288","DOI":"10.1038\/s41574-019-0176-8","volume":"15","author":"M Bl\u00fcher","year":"2019","unstructured":"Bl\u00fcher M. 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