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Rice is a good target for diet-related disease prevention strategies because it is widely consumed. Liu et al<jats:italic>.<\/jats:italic> (Proc Natl Acad Sci USA 115(44):11327\u201311332, 2018. <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/doi.org\/10.1073\/pnas.1806304115\">https:\/\/doi.org\/10.1073\/pnas.1806304115<\/jats:ext-link>) demonstrated that increasing the number of cell layers and thickness of putative aleurone in <jats:italic>ta2-1<\/jats:italic> (<jats:italic>thick aleurone 2-1<\/jats:italic>) mutant rice enhances simultaneously the content of multiple micronutrients. However, the increases of aleurone-associated nutrients were not proportional to the increases in the aleurone thickness. In this study, first, cytohistological analyses and transmission electron microscopy demonstrated that the multilayer in <jats:italic>ta2-1<\/jats:italic> exhibited aleurone cell structural features. Second, we detected an increase in insoluble fibre and insoluble bound-phenolic compounds, a shift in aleurone-specific neutral non-starch polysaccharide profile, enhancement of phytate and minerals such as iron, zinc, potassium, magnesium, sulphur, and manganese, enrichment of triacylglycerol and phosphatidylcholine but slight reduction in free fatty acid, and an increase in oleic fatty acid composition. These findings support our hypothesis that the expanded aleurone-like layers in <jats:italic>ta2-1<\/jats:italic> maintained some of the distinctive aleurone features and composition. We provide perspectives to achieve even greater filling of this expanded micronutrient sink to provide a means for multiple micronutrient enhancements in rice.<\/jats:p>","DOI":"10.1186\/s12284-021-00543-3","type":"journal-article","created":{"date-parts":[[2021,12,15]],"date-time":"2021-12-15T08:07:43Z","timestamp":1639555663000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Rice with Multilayer Aleurone: A Larger Sink for Multiple Micronutrients"],"prefix":"10.1186","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0523-168X","authenticated-orcid":false,"given":"Ronald","family":"Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoba","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinxin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Crispin A.","family":"Howitt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony R.","family":"Bird","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Ming","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip J.","family":"Larkin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,12,13]]},"reference":[{"key":"543_CR1","unstructured":"AOAC International (2016) Official methods of analysis of AOAC International (Association of Official Analytical Chemists International ed.), 20th ed. 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