{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T12:49:27Z","timestamp":1754398167810,"version":"3.37.3"},"reference-count":9,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2019,8,13]],"date-time":"2019-08-13T00:00:00Z","timestamp":1565654400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,8,13]],"date-time":"2019-08-13T00:00:00Z","timestamp":1565654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005633","name":"Syd\u00e4ntutkimuss\u00e4\u00e4ti\u00f6","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005633","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Finnish Academy Center of Excellence on Cardiovascular and Metabolic disease"},{"name":"European Research Council"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Mol and Cell Biol"],"published-print":{"date-parts":[[2019,12]]},"DOI":"10.1186\/s12860-019-0215-2","type":"journal-article","created":{"date-parts":[[2019,8,13]],"date-time":"2019-08-13T12:03:08Z","timestamp":1565697788000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Isolation of fresh endothelial cells from porcine heart for cardiovascular studies: a new fast protocol suitable for genomic, transcriptomic and cell biology studies"],"prefix":"10.1186","volume":"20","author":[{"given":"Olli-Pekka A.","family":"H\u00e4tinen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johanna E.","family":"L\u00e4hteenvuo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henna J.","family":"Korpela","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juho J.","family":"Pajula","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7593-2708","authenticated-orcid":false,"given":"Seppo","family":"Yl\u00e4-Herttuala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,8,13]]},"reference":[{"issue":"2","key":"215_CR1","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1161\/01.RES.0000255691.76142.4a","volume":"100","author":"WC Aird","year":"2007","unstructured":"Aird WC. Phenotypic heterogeneity of the endothelium: I. structure, function, and mechanisms. Circ Res. 2007;100(2):158\u201373.","journal-title":"Circ Res"},{"issue":"1","key":"215_CR2","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1093\/nar\/gkg061","volume":"31","author":"E Karsenty","year":"2003","unstructured":"Karsenty E, Barillot E, Tosser-Klopp G, et al. The GENETPIG database: a tool for comparative mapping in pig (Sus scrofa). Nucleic Acids Res. 2003;31(1):138\u201341.","journal-title":"Nucleic Acids Res"},{"issue":"6","key":"215_CR3","doi-asserted-by":"publisher","first-page":"1085","DOI":"10.1038\/nprot.2008.71","volume":"3","author":"JR van Beijnum","year":"2008","unstructured":"van Beijnum JR, Rousch M, Castermans K, van der Linden E, Griffioen AW. Isolation of endothelial cells from fresh tissues. Nat Protoc. 2008;3(6):1085\u201391.","journal-title":"Nat Protoc"},{"issue":"11","key":"215_CR4","doi-asserted-by":"publisher","first-page":"4164","DOI":"10.1182\/blood-2003-10-3537","volume":"103","author":"DA Lacorre","year":"2004","unstructured":"Lacorre DA, Baekkevold ES, Garrido I, et al. Plasticity of endothelial cells: rapid dedifferentiation of freshly isolated high endothelial venule endothelial cells outside the lymphoid tissue microenvironment. Blood. 2004;103(11):4164\u201372.","journal-title":"Blood"},{"issue":"11","key":"215_CR5","doi-asserted-by":"publisher","first-page":"2443","DOI":"10.1161\/ATVBAHA.107.141705","volume":"27","author":"MYK Lee","year":"2007","unstructured":"Lee MYK, Tse HF, Siu CW, Zhu SG, Man RYK, Vanhoutte PM. Genomic changes in regenerated porcine coronary arterial endothelial cells. Arterioscler Thromb Vasc Biol. 2007;27(11):2443\u20139.","journal-title":"Arterioscler Thromb Vasc Biol"},{"issue":"4","key":"215_CR6","doi-asserted-by":"publisher","first-page":"540","DOI":"10.3390\/microarrays4040540","volume":"4","author":"Michael Januszyk","year":"2015","unstructured":"Januszyk M, Rennert R, Sorkin M, et al. Evaluating the Effect of Cell Culture on Gene Expression in Primary Tissue Samples Using Microfluidic-Based Single Cell Transcriptional Analysis. Microarrays. 2015;4(4):540\u201350.","journal-title":"Microarrays"},{"issue":"3","key":"215_CR7","first-page":"187","volume":"12","author":"Marina Zaitseva","year":"2006","unstructured":"Zaitseva M, Vollenhoven BJ, Rogers PAW. In vitro culture significantly alters gene expression profiles and reduces differences between myometrial and fibroid smooth muscle cells. Mol Hum Reprod. 2006;12(3):187\u2013207.","journal-title":"MHR: Basic science of reproductive medicine"},{"issue":"2","key":"215_CR8","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1177\/0267659107079402","volume":"22","author":"B Neumann","year":"2007","unstructured":"Neumann B, Guo K, Paul A, et al. Perfusionists in the world of tissue engineering - a quick and easy method for safe isolation of huge quantities of porcine cardiomyocytes. Perfusion. 2007;22(2):137\u201342.","journal-title":"Perfusion"},{"key":"215_CR9","doi-asserted-by":"crossref","unstructured":"Decicco-skinner KL, Henry GH, Cataisson C, et al. Endothelial cell tube formation assay for the in vitro study of angiogenesis. J Vis Exp. 2014;10(91):1\u20138.","DOI":"10.3791\/51312"}],"container-title":["BMC Molecular and Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12860-019-0215-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s12860-019-0215-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12860-019-0215-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,11]],"date-time":"2020-08-11T23:08:26Z","timestamp":1597187306000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcmolcellbiol.biomedcentral.com\/articles\/10.1186\/s12860-019-0215-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,13]]},"references-count":9,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,12]]}},"alternative-id":["215"],"URL":"https:\/\/doi.org\/10.1186\/s12860-019-0215-2","relation":{},"ISSN":["2661-8850"],"issn-type":[{"type":"electronic","value":"2661-8850"}],"subject":[],"published":{"date-parts":[[2019,8,13]]},"assertion":[{"value":"3 January 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 August 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 August 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Not applicable","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"32"}}