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The use of zebrafish is a useful model system to study vertebrate gene expression and development. Here we characterize <jats:italic>znf143a<\/jats:italic>, a novel paralog of <jats:italic>znf143b<\/jats:italic>, previously known simply as <jats:italic>znf143<\/jats:italic> in zebrafish. This study reveals a comparison of quantitative and spatial expression patterns, transcriptional activity, and a knockdown analysis of both ZNF143 proteins.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>ZNF143a and ZNF143b have a fairly strong conservation with 65% amino acid sequence identity, and both are potent activators in transient transfection experiments. In situ hybridization analyses of both <jats:italic>znf143<\/jats:italic> mRNAs show that these genes are expressed strongly in regions of the brain at 24\u2009h post fertilization in zebrafish development. A transient knockdown analysis of <jats:italic>znf143<\/jats:italic> expression from either gene using CRISPR interference revealed similar morphological defects in brain development, and caused brain abnormalities in up to 50% of injected embryos. Although present in the same tissues, <jats:italic>znf143a<\/jats:italic> is expressed at a higher level in early development which might confer an evolutionary benefit for the maintenance of two paralogs in zebrafish.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p><jats:italic>znf143a<\/jats:italic> encodes a strong activator protein with high expression in neural tissues during early embryogenesis in zebrafish. Similar to its paralogous gene, <jats:italic>znf143b<\/jats:italic>, both <jats:italic>znf143<\/jats:italic> genes are required for normal development in zebrafish.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12860-020-0247-7","type":"journal-article","created":{"date-parts":[[2020,1,22]],"date-time":"2020-01-22T16:02:55Z","timestamp":1579708975000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Two paralogous znf143 genes in zebrafish encode transcriptional activator proteins with similar functions but expressed at different levels during early development"],"prefix":"10.1186","volume":"21","author":[{"given":"Laura","family":"Huning","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4199-369X","authenticated-orcid":false,"given":"Gary R.","family":"Kunkel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,1,22]]},"reference":[{"key":"247_CR1","doi-asserted-by":"publisher","first-page":"39953","DOI":"10.1074\/jbc.M608507200","volume":"281","author":"E Myslinski","year":"2006","unstructured":"Myslinski E, Gerard M-A, Krol A, Carbon P. 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