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Skin equivalent organotypic cultures can be engineered in vitro to study skin biology and wound closure by modelling the specific cellular components of the skin. This study aimed to develop a novel bioactive platelet-rich plasma (PRP) leukocyte depleted scaffold to facilitate the study of common clinical skin wounds in patients with poor chronic skin perfusion and low leukocyte infiltration. A scratch assay was performed on the skin model to mimic two skin wound conditions, an untreated condition and a condition treated with recombinant tumour necrotic factor (rTNF) to imitate the stimulation of an inflammatory state. Gene expression of <jats:italic>IL8<\/jats:italic> and <jats:italic>TGFA<\/jats:italic> was analysed in both conditions. Statistical analysis was done through ANOVA and paired student t-test. <jats:italic>P<\/jats:italic>\u00a0&lt;\u20090.05 was considered significant.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>A skin model that consisted of a leukocyte-depleted, platelet-rich plasma scaffold was setup with embedded fibroblasts as dermal equivalents and seeded keratinocytes as multi-layered epidermis. Gene expression levels of <jats:italic>IL8<\/jats:italic> and <jats:italic>TGFA<\/jats:italic> were significantly different between the control and scratched conditions (<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.001 and <jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.01 respectively), as well as between the control and treated conditions (p\u2009&lt;\u20090.01 and <jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.001 respectively). The scratch assay induced <jats:italic>IL8<\/jats:italic> upregulation after 3\u2009h (<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.05) which continued to increase up to day 1 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.05). On the other hand, the administration of TNF led to the downregulation of <jats:italic>IL8<\/jats:italic> (<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.01), followed by an upregulation on day 2. <jats:italic>IL8<\/jats:italic> gene expression decreased in the scratched condition after day 1 as the natural healing process took place and was lower than in the treated condition on day 8 (<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.05).<\/jats:p>\n                <jats:p>Both untreated and treated conditions showed a downregulation of <jats:italic>TGFA<\/jats:italic> 3\u2009h after scratch when compared with the control condition (<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.01). Administration of rTNF showed significant downregulation of <jats:italic>TGFA<\/jats:italic> after 24\u2009h when compared with the control (<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.01) and treated conditions (<jats:italic>p<\/jats:italic>\u00a0&lt;\u20090.05).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>This study suggests that a leukocyte-depleted PRP-based skin equivalent can be a useful model for the in vitro study of chronic skin wounds related to poor skin perfusion.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12860-021-00366-6","type":"journal-article","created":{"date-parts":[[2021,5,10]],"date-time":"2021-05-10T13:18:52Z","timestamp":1620652732000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Novel leukocyte-depleted platelet-rich plasma-based skin equivalent as an in vitro model of chronic wounds: a preliminary study"],"prefix":"10.1186","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3003-4151","authenticated-orcid":false,"given":"Elisa","family":"Seria","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Galea","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph","family":"Borg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin","family":"Schembri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriella","family":"Grech","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah Samut","family":"Tagliaferro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Felice","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,5,10]]},"reference":[{"issue":"1","key":"366_CR1","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1177\/000331979704800111","volume":"48","author":"CV Ruckley","year":"1997","unstructured":"Ruckley CV. 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