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However, the single-cell molecular architecture of MCs and the key molecular events during the differentiation remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We performed single-cell RNA sequencing (RNA sequencing) and single-cell assay for transposase-accessible chromatin (ATAC-Seq) to analyze the developmental features of MCs to heart, kidney, spleen, liver, and brain in human embryos at gestational ages 7\u201317 weeks.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We found that\n                      <jats:italic>EGR1<\/jats:italic>\n                      might be relevant to the differentiation of heterogeneous MC sub-clusters. Meanwhile,\n                      <jats:italic>RPL10P9<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>+<\/jats:italic>\n                      <\/jats:sup>\n                      <jats:italic>PTMAP5<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>+<\/jats:italic>\n                      <\/jats:sup>\n                      MCs had the closest expression profiling with endocardial cells.\n                      <jats:italic>NDUFA4L2<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>+<\/jats:italic>\n                      <\/jats:sup>\n                      <jats:italic>A2M<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>+<\/jats:italic>\n                      <\/jats:sup>\n                      MCs presented the potentials to form endothelial cells (ECs) and hematopoietic stem cells, and\n                      <jats:italic>MEF2C<\/jats:italic>\n                      might be involved in this process.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings provide insights into the molecular architecture and lineage progression of MCs during early human embryonic organogenesis, offering a valuable reference for regenerative medicine and organ bioengineering.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12860-025-00561-9","type":"journal-article","created":{"date-parts":[[2025,12,25]],"date-time":"2025-12-25T16:32:55Z","timestamp":1766680375000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular architecture of mesoderm cells across early to middle stage of human embryo development at single-cell resolution"],"prefix":"10.1186","volume":"27","author":[{"given":"Wei","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haiyan","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhibin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dandan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yane","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenting","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenlong","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhipeng","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinqiong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhanye","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minglin","family":"Ou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donge","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Dai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,12,25]]},"reference":[{"issue":"7\u20138","key":"561_CR1","doi-asserted-by":"publisher","first-page":"531","DOI":"10.1079\/IVP2002371","volume":"47","author":"U Technau","year":"2003","unstructured":"Technau U, Scholz CB. 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