{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T01:45:30Z","timestamp":1793065530720,"version":"4.5.0"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T00:00:00Z","timestamp":1769040000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T00:00:00Z","timestamp":1771200000000},"content-version":"vor","delay-in-days":25,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100004951","name":"Atat\u00fcrk \u00dcniversitesi","doi-asserted-by":"publisher","award":["TDK-2020-8498)"],"award-info":[{"award-number":["TDK-2020-8498)"]}],"id":[{"id":"10.13039\/501100004951","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Mol and Cell Biol"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Irisin is a myokine that is secreted by muscle tissue and is recognized for its regulatory effects on inflammation. The objective of this study was to examine the potential anti-inflammatory function of irisin in the context of colon cancer, with a focus on its effects on the Caco-2 cell line. Method: Specifically, pro-inflammatory cytokine levels (IL-6 and TNF-\u03b1), apoptotic marker Caspase-3 activity, and changes in the NF-\u03baB signaling pathway were assessed using ELISA assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The findings of this study indicated a substantial augmentation in IL-6 levels in the 100 nM irisin-treated group in comparison to the control group (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). Furthermore, a significant divergence in Caspase-3 activity was observed between the 10 nM and 100 nM irisin groups (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05). While direct antiproliferative effects of irisin on Caco-2 cells were not evident in analyses of cellular toxicity, a significant increase in Caspase-3 activity suggested activation of apoptotic pathways. Furthermore, the upregulation of NF-\u03baB signaling, which is generally linked to cancer progression through the suppression of apoptosis, was observed following irisin treatment.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Irisin did not exert a consistent antiproliferative effect in Caco-2 cells under the tested conditions. Instead, irisin exposure was associated with alterations in inflammatory and apoptotic markers, including increased IL-6 and NF-\u03baB levels and modulation of caspase-3 activity. These findings suggest that irisin may act as a context-dependent regulator of inflammatory and apoptotic signaling rather than a direct anti-inflammatory or antiproliferative agent in colon cancer cells.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12860-026-00568-w","type":"journal-article","created":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T02:05:45Z","timestamp":1769047545000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effects of irisin on inflammatory and apoptotic markers in the Caco-2 colon cancer cell line"],"prefix":"10.1186","volume":"27","author":[{"given":"Elif Zeynep","family":"Ozturk","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ebubekir","family":"Bakan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurcan Kilic","family":"Baygutalp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zafer","family":"Bayraktutan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,1,22]]},"reference":[{"issue":"1","key":"568_CR1","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1152\/physrev.00015.2003","volume":"84","author":"B Cannon","year":"2004","unstructured":"Cannon B, Nedergaard J. 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