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Immunofluorescence imaging of ciliary substructures is a key tool in cilia research, but multiplex staining is often limited by the availability of antibodies from different host species.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We developed a novel goat-derived gamma tubulin antibody for basal body labelling and validated its specificity through triple-label immunofluorescence in different cell lines.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The antibody showed strong, specific staining of basal bodies under methanol fixation, enabling clear visualization in combination with rabbit- and mouse-derived antibodies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This antibody expands multiplex imaging capabilities for ciliary substructures, supporting both cilia research and diagnostics of ciliopathies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12860-026-00595-7","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T08:25:27Z","timestamp":1779956727000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A goat-derived gamma-tubulin antibody for triple-channel imaging of primary cilia"],"prefix":"10.1186","volume":"27","author":[{"given":"Eliza","family":"Karapetian","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Gerhardt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Lemanska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monia","family":"Budnik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Stahl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas","family":"F\u00fcner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Hirschberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thorsten","family":"Pfirrmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,5,28]]},"reference":[{"issue":"1","key":"595_CR1","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1186\/2046-2530-1-7","volume":"1","author":"HL May-Simera","year":"2012","unstructured":"May-Simera HL, Kelley MW. 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The animal experimentation has been approved by the Institutional Review Board of preclincs GmbH, as well as the competent authorities. We confirm the compliance with all relevant guidelines, especially Directive 2010\/63\/EU, the German Animal Welfare Act (TierSchG) and the Animal Welfare Experimental Animal Ordinance (TierSchVersV). The animals were privately owned by Behring Campus Eystrup GmbH which is approved by the competent authorities as an official source for experimental animals. We can confirm that we obtained informed consent from the owner. 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