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However, the dazzling morphological differentiations have caused numerous systematic confusion within the big genus, especially the puzzlingly generic taxonomy. So far, there is lack of molecular phylogenetic study to address the chaotic situation. In this study, we undertook mitochondrial genome sequencing of 42 representative species including 18 newly-sequenced ones from Eastern Asia and reconstructed the phylogenetic framework of stag beetles in <jats:italic>Dorcus<\/jats:italic> sensu lato for the first time.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The mitogenome datasets of <jats:italic>Dorcus<\/jats:italic> species have indicated the variable mitogenomic lengths ranged from 15,785 to 19,813\u00a0bp. Each mitogenome contained 13 PCGs, 2 rRNAs, 22 tRNAs, and a control region, and all PCGs were under strong purifying selection (Ka\/Ks\u2009&lt;\u20091). Notably, we have identified the presence of a substantial intergenic spacer (IGS) between the <jats:italic>trnA<\/jats:italic><jats:sup><jats:italic>ser (UCN)<\/jats:italic><\/jats:sup> and <jats:italic>NAD1<\/jats:italic> genes, with varying lengths ranging from 129\u00a0bp (in <jats:italic>D. hansi<\/jats:italic>) to 158\u00a0bp (in <jats:italic>D. tityus<\/jats:italic>). The mitogenomic phylogenetic analysis of 42 species showed that Eastern Asia <jats:italic>Dorcus<\/jats:italic> was monophyletic, and divided into eight clades with significant genetic distance. Four of them, Clade VIII, VII, VI and I are clustered by the representative species of <jats:italic>Serrognathus<\/jats:italic> Motschulsky, <jats:italic>Kirchnerius<\/jats:italic> Schenk, <jats:italic>Falcicornis<\/jats:italic> S\u00e9guy and <jats:italic>Dorcus<\/jats:italic> s.s. respectively, which supported their fully generic positions as the previous morphological study presented. The topology also showed the remaining clades were distinctly separated from the species of <jats:italic>Dorcus<\/jats:italic> sensu lato, which implied that each of them might demonstrate independent generic status. The Linnaeus nomenclatures were suggested as <jats:italic>Eurydorcus<\/jats:italic> Didier stat. res., <jats:italic>Eurytrachellelus<\/jats:italic> Didier stat. res., <jats:italic>Hemisodorcus<\/jats:italic> Thomson stat. res. and <jats:italic>Velutinodorcus<\/jats:italic> Maes stat. res. For Clade V, IV, III and II respectively.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>This study recognized the monophyly of <jats:italic>Dorcus<\/jats:italic> stag beetles and provided a framework for the molecular phylogeny of this group for the first time. The newly generated mitogenomic data serves as a valuable resource for future investigations on lucanid beetles. The generic relationship would facilitate the systematics of <jats:italic>Dorcus<\/jats:italic> stag beetles and thus be useful for exploring their evolutionary, ecological, and conservation aspects.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12862-024-02225-2","type":"journal-article","created":{"date-parts":[[2024,5,21]],"date-time":"2024-05-21T07:02:06Z","timestamp":1716274926000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The first mitogenomic phylogenetic framework of Dorcus sensu lato (Coleoptera: Lucanidae), with an emphasis on generic taxonomy in Eastern Asia"],"prefix":"10.1186","volume":"24","author":[{"given":"Muhammad","family":"Jafir","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyang","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjing","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Wan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,5,21]]},"reference":[{"key":"2225_CR1","first-page":"418","volume":"43","author":"X Wan","year":"2006","unstructured":"Wan X, Yang X. 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