{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T08:28:34Z","timestamp":1786350514968,"version":"build-2736575974"},"reference-count":53,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":5,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Animal and Plant Quarantine Agency, Ministry of Agriculture, Food, and Rural Affairs (MAFRA), Republic of Korea","award":["B-1543073-2024-26-01"],"award-info":[{"award-number":["B-1543073-2024-26-01"]}]},{"name":"Animal and Plant Quarantine Agency, Ministry of Agriculture, Food, and Rural Affairs (MAFRA), Republic of Korea","award":["B-1543073-2024-26-01"],"award-info":[{"award-number":["B-1543073-2024-26-01"]}]},{"name":"Animal and Plant Quarantine Agency, Ministry of Agriculture, Food, and Rural Affairs (MAFRA), Republic of Korea","award":["B-1543073-2024-26-01"],"award-info":[{"award-number":["B-1543073-2024-26-01"]}]},{"name":"Animal and Plant Quarantine Agency, Ministry of Agriculture, Food, and Rural Affairs (MAFRA), Republic of Korea","award":["B-1543073-2024-26-01"],"award-info":[{"award-number":["B-1543073-2024-26-01"]}]},{"name":"Animal and Plant Quarantine Agency, Ministry of Agriculture, Food, and Rural Affairs (MAFRA), Republic of Korea","award":["B-1543073-2024-26-01"],"award-info":[{"award-number":["B-1543073-2024-26-01"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Genom Data"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Production of high-quality biological products depends on the careful selection and consistent management of seed strains. In this study, we generated complete genome sequences of bacterial strains employed in inactivated animal vaccines in South Korea and comprehensively examined their gene annotations. The genome data thus obtained provide a solid basis for confirming, at the strain level, whether manufacturers are using the same seed strains over time. In addition, these data offer a useful reference resource to support stable large-scale production and quality control of biological products.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Data description<\/jats:title>\n                    <jats:p>\n                      The complete genomes of eleven bacterial strains employed for inactivated vaccine production were generated and annotated. A hybrid assembly workflow combining Illumina short reads (NovaSeq 6000) with Oxford Nanopore long reads (MinION) produced high-quality, gap-free genomes for all strains, with genome sizes ranging from 2.28 Mb to 5.35 Mb. The strains comprised\n                      <jats:italic>Pasteurella multocida<\/jats:italic>\n                      (D, 3A, A),\n                      <jats:italic>Actinobacillus pleuropneumoniae<\/jats:italic>\n                      (2 and 5),\n                      <jats:italic>Glaesserella parasuis<\/jats:italic>\n                      4,\n                      <jats:italic>Mannheimia haemolytica<\/jats:italic>\n                      KO,\n                      <jats:italic>Avibacterium paragallinarum<\/jats:italic>\n                      C, and three\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      strains (F41, YC21-F17, K99S). All assemblies exhibited high completeness (&gt;99%) and minimal contamination (&lt;1%), ensuring reliable downstream genomic characterization.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12863-026-01475-x","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T14:06:41Z","timestamp":1785938801000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High-quality genome assembly and annotation of inactivated animal vaccine bacteria strains in South Korea"],"prefix":"10.1186","volume":"27","author":[{"given":"Yeonkyeong","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Ju","family":"Nah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su-Min","family":"Go","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun-Ok","family":"Ku","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Il","family":"Jang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,8,5]]},"reference":[{"key":"1475_CR1","doi-asserted-by":"publisher","unstructured":"Sheets R. & Petricciani, J. Vaccine cell substrates 2004. Expert Rev vVaccines. 2004. https:\/\/doi.org\/10.1586\/14760584.3.6.633","DOI":"10.1586\/14760584.3.6.633"},{"key":"1475_CR2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.07.005","author":"T Horimoto","year":"2006","unstructured":"Horimoto T et al. The development and characterization of H5 influenza virus vaccines derived from a 2003 human isolate. Vaccine. 2006. https:\/\/doi.org\/10.1016\/j.vaccine.2005.07.005","journal-title":"Vaccine"},{"key":"1475_CR3","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.04259-23","author":"K Krysztopa-Grzybowska","year":"2024","unstructured":"Krysztopa-Grzybowska K, et al. The whole genome sequence of Polish vaccine strain Mycobacterium bovis BCG Moreau. 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Data set 11. Plasmid p5_1 sequence of Actinobacillus pleuropneumoniae 5. NCBI. 2026.  https:\/\/identifiers.org\/ncbi\/insdc:JBSQGB020000002"},{"key":"1475_CR29","unstructured":"Lee Y. Data set 12. Genome assembly of Actinobacillus pleuropneumoniae 5. NCBI. 2026. https:\/\/identifiers.org\/insdc.gca:GCA_054171605"},{"key":"1475_CR30","unstructured":"Lee Y. Data set 13. Chromosome sequence of Glaesserella parasuis 4. NCBI. 2026. https:\/\/identifiers.org\/insdc:CM135138"},{"key":"1475_CR31","unstructured":"Lee Y. Data set 14. Genome assembly of Glaesserella parasuis 4. NCBI. 2026. https:\/\/identifiers.org\/insdc.gca:GCA_054171585"},{"key":"1475_CR32","unstructured":"Lee Y. Data set 15. Chromosome sequence of Pasteurella multocida A. NCBI. 2026. https:\/\/identifiers.org\/insdc:CM135137"},{"key":"1475_CR33","unstructured":"Lee Y. Data set 16. Genome assembly of Pasteurella multocida A. 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NCBI. 2026. https:\/\/identifiers.org\/ncbi\/insdc:JBSQGG020000002"},{"key":"1475_CR40","unstructured":"Lee Y. Data set 23. Plasmid pF41_2 sequence of Escherichia coli F41. NCBI. 2026. https:\/\/identifiers.org\/ncbi\/insdc:JBSQGG020000003"},{"key":"1475_CR41","unstructured":"Lee Y. Data set 24. Plasmid pF41_3 sequence of Escherichia coli F41. NCBI. 2026. https:\/\/identifiers.org\/ncbi\/insdc:JBSQGG020000004"},{"key":"1475_CR42","unstructured":"Lee Y. Data set 25. Plasmid pF41_4 sequence of Escherichia coli F41. NCBI. 2026. https:\/\/identifiers.org\/ncbi\/insdc:JBSQGG020000005"},{"key":"1475_CR43","unstructured":"Lee Y. Data set 26. Genome assembly of Escherichia coli F41. NCBI. 2026. https:\/\/identifiers.org\/insdc.gca:GCA_054171505"},{"key":"1475_CR44","unstructured":"Lee Y. Data set 27. Chromosome sequence of Escherichia coli YC21-F17. NCBI. 2026.  https:\/\/identifiers.org\/insdc:CM176886"},{"key":"1475_CR45","unstructured":"Lee Y. Data set 28. Plasmid pYC21-F17_1 sequence of Escherichia coli YC21-F17. NCBI. 2026. https:\/\/identifiers.org\/ncbi\/insdc:JBYUJG010000002"},{"key":"1475_CR46","unstructured":"Lee Y. Data set 29. Plasmid pYC21-F17_2 sequence of Escherichia coli YC21-F17. NCBI. 2026.  https:\/\/identifiers.org\/ncbi\/insdc:JBYUJG010000003"},{"key":"1475_CR47","unstructured":"Lee Y. Data set 30. Plasmid pYC21-F17_3 sequence of Escherichia coli YC21-F17. NCBI. 2026.  https:\/\/identifiers.org\/ncbi\/insdc:JBYUJG010000004"},{"key":"1475_CR48","unstructured":"Lee Y. Data set 31. Genome assembly of Escherichia coli YC21-F17. NCBI. 2026. https:\/\/identifiers.org\/insdc.gca:GCA_058136955"},{"key":"1475_CR49","unstructured":"Lee Y. Data set 32. Chromosome sequence of Escherichia coli K99S. NCBI. 2026. https:\/\/identifiers.org\/insdc:CM135132"},{"key":"1475_CR50","unstructured":"Lee Y. Data set 33. Plasmid pK99S_1 sequence of Escherichia coli K99S. 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NCBI. 2026. https:\/\/identifiers.org\/insdc.gca:GCA_054171465"}],"container-title":["BMC Genomic Data"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12863-026-01475-x","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12863-026-01475-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12863-026-01475-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T07:39:55Z","timestamp":1786347595000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1186\/s12863-026-01475-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,5]]},"references-count":53,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,12]]}},"alternative-id":["1475"],"URL":"https:\/\/doi.org\/10.1186\/s12863-026-01475-x","relation":{},"ISSN":["2730-6844"],"issn-type":[{"value":"2730-6844","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,5]]},"assertion":[{"value":"9 March 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 July 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 August 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Not applicable.","order":1,"name":"Ethics","label":"Ethics approval and consent to participate","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Not applicable.","order":2,"name":"Ethics","label":"Consent for publication","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"65"}}