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The organisms have evolved sophisticated defense system to alleviate heavy metal toxicity. Hydrogen sulfide (H\n                      <jats:sub>2<\/jats:sub>\n                      S) effectively alleviates heavy metal toxicity in plants and reduces oxidative stress in mammals. However, the function of H\n                      <jats:sub>2<\/jats:sub>\n                      S for alleviating heavy metal toxicity in aquatic organisms remains less clear.\n                      <jats:italic>Tetrahymena thermophila<\/jats:italic>\n                      is an important model organism to evaluate toxic contaminants in an aquatic environment. In this study, the molecular roles of exogenously H\n                      <jats:sub>2<\/jats:sub>\n                      S application were explored by RNA sequencing under Cd stress in\n                      <jats:italic>T. thermophila<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The exposure of 30\u2009\u03bcM Cd resulted in\n                      <jats:italic>T. thermophila<\/jats:italic>\n                      growth inhibition, cell nigrescence, and malondialdehyde (MDA) content considerably increase. However, exogenous NaHS (donor of H\n                      <jats:sub>2<\/jats:sub>\n                      S, 70\u2009\u03bcM) significantly alleviated the Cd-induced toxicity by inhibiting Cd absorbtion, promoting CdS nanoparticles formation and improving antioxidant system. Comparative transcriptome analysis showed that the expression levels of 9152 genes changed under Cd stress (4658 upregulated and 4494 downregulated). However, only 1359 genes were differentially expressed with NaHS treatment under Cd stress (1087 upregulated and 272 downregulated). The functional categories of the differentially expressed genes (DEGs) by gene ontology (GO) revealed that the transcripts involved in the oxidation\u2013reduction process, oxidoreductase activity, glutathione peroxidase activity, and cell redox homeostasis were the considerable enrichments between Cd stress and NaHS treatment under Cd stress. Kyoto Encyclopedia of Genes and Genomes (KEGG) indicated that the carbon metabolism, glutathione metabolism, metabolism of xenobiotics by cytochrome P450, and ABC transporters were significantly differentially expressed components between Cd stress and NaHS treatment under Cd stress in\n                      <jats:italic>T. thermophila<\/jats:italic>\n                      . The relative expression levels of six DEGs were further confirmed through quantitative real-time polymerase chain reaction (qRT-PCR).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      NaHS alleviated Cd stress mainly through inhibiting Cd absorbtion, promoting CdS nanoparticles formation, increasing oxidation resistance, and regulation of transport in free-living unicellular\n                      <jats:italic>T. thermophila<\/jats:italic>\n                      . These findings will expand our understanding for H\n                      <jats:sub>2<\/jats:sub>\n                      S functions in the freshwater protozoa.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12864-020-07337-9","type":"journal-article","created":{"date-parts":[[2021,1,6]],"date-time":"2021-01-06T15:05:26Z","timestamp":1609945526000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Comparative transcriptome analysis uncovers roles of hydrogen sulfide for alleviating cadmium toxicity in Tetrahymena thermophila"],"prefix":"10.1186","volume":"22","author":[{"given":"Hongrui","family":"Lv","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Bo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1595-9797","authenticated-orcid":false,"given":"Wei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,1,6]]},"reference":[{"key":"7337_CR1","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1007\/s00344-017-9730-6","volume":"37","author":"P Ahmad","year":"2018","unstructured":"Ahmad P, Ahanger MA, Egamberdieva D, Alam P, Alyemeni MN, Ashraf M. 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