{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T14:46:17Z","timestamp":1790606777137,"version":"4.1.0"},"reference-count":43,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,1,17]],"date-time":"2020-01-17T00:00:00Z","timestamp":1579219200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,17]],"date-time":"2020-01-17T00:00:00Z","timestamp":1579219200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Genomics"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Reproductive capacity can be altered by challenges experienced during critical periods of development, including fetal development and early neonatal life. Gossypol is a polyphenolic compound, commonly found in cotton seeds, that impairs male reproduction. Here, we investigated whether the exposure to gossypol in utero and during lactation alters male reproductive function in sheep. From conception until 60\u2009days postpartum, ewes were randomly assigned to a control diet or a gossypol-rich diet based on cottonseed. Lamb testicles were removed at 60\u2009days of age and subjected to RNA-sequencing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Lambs derived from the maternal cottonseed diet showed significantly lower growth and lower testis weight as a proportion of the total body weight, and reduced testosterone levels. In addition, the testis transcriptome was significantly altered by the maternal cottonseed diet. Most of the altered genes are directly implicated in testis development and sperm biology, cell communication, iron ion metabolism, calcium homeostasis and signaling, among other functions. Interestingly, network analysis revealed that exposure to gossypol significantly disturbed coexpression patterns among spermatogenesis-related genes, suggesting a disruption in coregulation mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings provide evidence that maternal exposure to gossypol alters male reproductive function in the offspring, with potential lasting or lifelong negative consequences.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12864-020-6487-2","type":"journal-article","created":{"date-parts":[[2020,1,17]],"date-time":"2020-01-17T09:02:50Z","timestamp":1579251770000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Gestational and lactational exposure to gossypol alters the testis transcriptome"],"prefix":"10.1186","volume":"21","author":[{"given":"Helder","family":"Louvandini","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia S.","family":"Corr\u00eaa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roc\u00edo","family":"Amor\u00edn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihe","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Egon H.","family":"Ieda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina R.","family":"Jimenez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siu M.","family":"Tsai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Concepta M.","family":"McManus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6661-3991","authenticated-orcid":false,"given":"Francisco","family":"Pe\u00f1agaricano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,1,17]]},"reference":[{"key":"6487_CR1","first-page":"231635","volume":"2014","author":"IC Gadelha","year":"2014","unstructured":"Gadelha IC, Fonseca NB, Oloris SC, Melo MM, Soto-Blanco B. Gossypol toxicity from cottonseed products. Sci World J. 2014;2014:231635.","journal-title":"Sci World J"},{"issue":"7","key":"6487_CR2","doi-asserted-by":"publisher","first-page":"1168","DOI":"10.3382\/ps.0741168","volume":"74","author":"SR Fernandez","year":"1995","unstructured":"Fernandez SR, Zhang Y, Parsons CM. Dietary formulation with cottonseed meal on a total amino acid versus a digestible amino acid basis. Poult Sci. 1995;74(7):1168\u201379.","journal-title":"Poult Sci"},{"issue":"1","key":"6487_CR3","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/0024-3205(85)90623-X","volume":"37","author":"YC Lin","year":"1985","unstructured":"Lin YC, Fukaya T, Rikihisa Y, Walton A. Gossypol in female fertility control: ovum implantation and early pregnancy inhibited in rats. Life Sci. 1985;37(1):39\u201347.","journal-title":"Life Sci"},{"issue":"6","key":"6487_CR4","doi-asserted-by":"publisher","first-page":"469","DOI":"10.3109\/01480548509041070","volume":"8","author":"RK Lagerl\u00f6f","year":"1985","unstructured":"Lagerl\u00f6f RK, Tone JN. The effect of gossypol acetic acid on female reproduction. Drug Chem Toxicol. 1985;8(6):469\u201382.","journal-title":"Drug Chem Toxicol"},{"issue":"5","key":"6487_CR5","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1016\/0093-691X(96)00021-0","volume":"45","author":"R Randel","year":"1996","unstructured":"Randel R, Willard S, Wyse S, French L. Effects of diets containing free gossypol on follicular development, embryo recovery and corpus luteum function in Brangus heifers treated with bFSH. Theriogenology. 1996;45(5):911\u201322.","journal-title":"Theriogenology"},{"issue":"1","key":"6487_CR6","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/0010-7824(81)90072-X","volume":"24","author":"D Hahn","year":"1981","unstructured":"Hahn D, Rusticus C, Probst A, Homm R, Johnson A. Antifertility and endocrine activities of gossypol in rodents. Contraception. 1981;24(1):97\u2013105.","journal-title":"Contraception"},{"issue":"3","key":"6487_CR7","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1016\/0300-483X(86)90060-0","volume":"40","author":"R Heywood","year":"1986","unstructured":"Heywood R, Lloyd G, Majeed S, Gopinath C. The toxicity of gossypol to the male rat. Toxicology. 1986;40(3):279\u201384.","journal-title":"Toxicology"},{"issue":"4","key":"6487_CR8","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1016\/0300-483X(84)90084-2","volume":"32","author":"M G\u00e5fvels","year":"1984","unstructured":"G\u00e5fvels M, Wang J, Bergh A, Damber J-E, Selstam G. Toxic effects of the antifertility agent gossypol in male rats. Toxicology. 1984;32(4):325\u201333.","journal-title":"Toxicology"},{"issue":"12","key":"6487_CR9","doi-asserted-by":"publisher","first-page":"3354","DOI":"10.1016\/j.fct.2010.09.004","volume":"48","author":"A El-Sharaky","year":"2010","unstructured":"El-Sharaky A, Newairy A, Elguindy N, Elwafa A. Spermatotoxicity, biochemical changes and histological alteration induced by gossypol in testicular and hepatic tissues of male rats. Food Chem Toxicol. 2010;48(12):3354\u201361.","journal-title":"Food Chem Toxicol"},{"issue":"1","key":"6487_CR10","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/0093-691X(94)90657-5","volume":"42","author":"P Chenoweth","year":"1994","unstructured":"Chenoweth P, Risco C, Larsen R, Velez J, Tran T, Chase C Jr. Effects of dietary gossypol on aspects of semen quality, sperm morphology and sperm production in young Brahman bulls. Theriogenology. 1994;42(1):1\u201313.","journal-title":"Theriogenology"},{"issue":"6","key":"6487_CR11","doi-asserted-by":"publisher","first-page":"1171","DOI":"10.1016\/j.theriogenology.2003.07.007","volume":"61","author":"ME Hassan","year":"2004","unstructured":"Hassan ME, Smith GW, Ott RS, Faulkner DB, Firkins LD, Ehrhart E, Schaeffer DJ. Reversibility of the reproductive toxicity of gossypol in peripubertal bulls. Theriogenology. 2004;61(6):1171\u20139.","journal-title":"Theriogenology"},{"issue":"5","key":"6487_CR12","doi-asserted-by":"publisher","first-page":"1628","DOI":"10.2527\/1992.7051628x","volume":"70","author":"R Randel","year":"1992","unstructured":"Randel R, Chase C Jr, Wyse S. Effects of gossypol and cottonseed products on reproduction of mammals. J Anim Sci. 1992;70(5):1628\u201338.","journal-title":"J Anim Sci"},{"issue":"1","key":"6487_CR13","doi-asserted-by":"publisher","first-page":"538","DOI":"10.1016\/j.mce.2013.09.008","volume":"382","author":"E Zambrano","year":"2014","unstructured":"Zambrano E, Guzman C, Rodriguez-Gonzalez GL, Durand-Carbajal M, Nathanielsz PW. Fetal programming of sexual development and reproductive function. Mol Cell Endocrinol. 2014;382(1):538\u201349.","journal-title":"Mol Cell Endocrinol"},{"key":"6487_CR14","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1186\/1477-7827-9-94","volume":"9","author":"FC Toledo","year":"2011","unstructured":"Toledo FC, Perobelli JE, Pedrosa FP, Anselmo-Franci JA, Kempinas WD. In utero protein restriction causes growth delay and alters sperm parameters in adult male rats. Reprod Biol Endocrinol. 2011;9:94.","journal-title":"Reprod Biol Endocrinol"},{"issue":"2","key":"6487_CR15","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1677\/joe.1.06712","volume":"192","author":"CV Teixeira","year":"2007","unstructured":"Teixeira CV, Silandre D, de Souza Santos AM, Delalande C, Sampaio FJ, Carreau S, da Fonte RC. Effects of maternal undernutrition during lactation on aromatase, estrogen, and androgen receptors expression in rat testis at weaning. J Endocrinol. 2007;192(2):301\u201311.","journal-title":"J Endocrinol"},{"key":"6487_CR16","first-page":"837","volume-title":"Schalm\u2019s Veterinary Hematology","author":"SR Byers","year":"2010","unstructured":"Byers SR, Kramer JW. Normal hematology of sheep and goats. In: Weiss DJ, Wardrop KJ, editors. Schalm\u2019s Veterinary Hematology. Ames: Wiley-Blackwell; 2010. p. 837."},{"issue":"2","key":"6487_CR17","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/0010-7824(85)90030-7","volume":"31","author":"A Donaldson","year":"1985","unstructured":"Donaldson A, Sufi S, Jeffcoate S. Inhibition by gossypol of testosterone production by mouse Leydig cells in vitro. Contraception. 1985;31(2):165\u201371.","journal-title":"Contraception"},{"issue":"5","key":"6487_CR18","doi-asserted-by":"publisher","first-page":"683","DOI":"10.1016\/0022-4731(86)90011-7","volume":"25","author":"S Pearce","year":"1986","unstructured":"Pearce S, Sufi S, O'Shaughnessy P, Donaldson A, Jeffcoate S. Site of gossypol inhibition of steroidogenesis in purified mouse Leydig cells. J Steroid Biochem. 1986;25(5):683\u20137.","journal-title":"J Steroid Biochem"},{"issue":"1","key":"6487_CR19","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1093\/molehr\/8.1.8","volume":"8","author":"A Eriksson","year":"2002","unstructured":"Eriksson A, T\u00f6h\u00f6nen V, Wedell A, Nordqvist K. Isolation of the human testatin gene and analysis in patients with abnormal gonadal development. Mol Hum Reprod. 2002;8(1):8\u201315.","journal-title":"Mol Hum Reprod"},{"issue":"1","key":"6487_CR20","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/S0303-7207(01)00666-9","volume":"184","author":"C Olesen","year":"2001","unstructured":"Olesen C, Larsen NJ, Byskov AG, Harboe TL, Tommerup N. Human FATE is a novel X-linked gene expressed in fetal and adult testis. Mol Cell Endocrinol. 2001;184(1):25\u201332.","journal-title":"Mol Cell Endocrinol"},{"issue":"6","key":"6487_CR21","doi-asserted-by":"publisher","first-page":"2149","DOI":"10.1016\/S0002-9440(10)63571-7","volume":"163","author":"RI Skotheim","year":"2003","unstructured":"Skotheim RI, Korkmaz KS, Klokk TI, Abeler VM, Korkmaz CG, Nesland JM, Fossa SD, Lothe RA, Saatcioglu F. NKX3.1 expression is lost in testicular germ cell tumors. Am J Pathol. 2003;163(6):2149\u201354.","journal-title":"Am J Pathol"},{"issue":"1","key":"6487_CR22","doi-asserted-by":"publisher","first-page":"378","DOI":"10.1006\/dbio.1995.1152","volume":"169","author":"TG Wolfsberg","year":"1995","unstructured":"Wolfsberg TG, Straight PD, Gerena RL, Huovila A-PJ, Primakoff P, Myles DG, White JM. ADAM, a widely distributed and developmentally regulated gene family encoding membrane proteins with a disintegrin and metalloprotease domain. Dev Biol. 1995;169(1):378\u201383.","journal-title":"Dev Biol"},{"issue":"2","key":"6487_CR23","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1083\/jcb.131.2.275","volume":"131","author":"TG Wolfsberg","year":"1995","unstructured":"Wolfsberg TG, Primakoff P, Myles DG, White JM. ADAM, a novel family of membrane proteins containing a Disintegrin and Metalloprotease domain: multipotential functions in cell-cell and cell-matrix interactions. J Cell Biol. 1995;131(2):275\u20138.","journal-title":"J Cell Biol"},{"issue":"5","key":"6487_CR24","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1007\/s10059-010-0138-4","volume":"30","author":"E Kim","year":"2010","unstructured":"Kim E, Lee Y, Kim JS, Song BS, Kim SU, Huh JW, Lee SR, Kim SH, Hong Y, Chang KT. Extracellular domain of V-set and immunoglobulin domain containing 1 (VSIG1) interacts with sertoli cell membrane protein, while its PDZ-binding motif forms a complex with ZO-1. Mol Cell. 2010;30(5):443\u20138.","journal-title":"Mol Cell"},{"issue":"2","key":"6487_CR25","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1095\/biolreprod.116.145458","volume":"96","author":"T Iwamori","year":"2017","unstructured":"Iwamori T, Iwamori N, Matsumoto M, Ono E, Matzuk MM. Identification of KIAA1210 as a novel X-chromosome-linked protein that localizes to the acrosome and associates with the ectoplasmic specialization in testes. Biol Reprod. 2017;96(2):469\u201377.","journal-title":"Biol Reprod"},{"issue":"1","key":"6487_CR26","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1006\/abbi.2001.2322","volume":"389","author":"J Bylund","year":"2001","unstructured":"Bylund J, Oliw EH. Cloning and characterization of CYP4F21: a prostaglandin E2 20-hydroxylase of ram seminal vesicles. Arch Biochem Biophys. 2001;389(1):123\u20139.","journal-title":"Arch Biochem Biophys"},{"issue":"2","key":"6487_CR27","doi-asserted-by":"publisher","first-page":"803","DOI":"10.1152\/physrev.00023.2012","volume":"93","author":"MD Parker","year":"2013","unstructured":"Parker MD, Boron WF. The divergence, actions, roles, and relatives of sodium-coupled bicarbonate transporters. Physiol Rev. 2013;93(2):803\u2013959.","journal-title":"Physiol Rev"},{"key":"6487_CR28","doi-asserted-by":"publisher","first-page":"56","DOI":"10.3389\/fcell.2014.00056","volume":"2","author":"A Allais-Bonnet","year":"2014","unstructured":"Allais-Bonnet A, Pailhoux E. Role of the prion protein family in the gonads. Front Cell Dev Biol. 2014;2:56.","journal-title":"Front Cell Dev Biol"},{"issue":"7","key":"6487_CR29","doi-asserted-by":"publisher","first-page":"2075","DOI":"10.1021\/acs.jafc.8b06946","volume":"67","author":"W Lim","year":"2019","unstructured":"Lim W, Ham J, Park S, Bae H, You S, Song G. Gossypol induces disruption of spermatogenesis and Steroidogenesis in male mice. J Agric Food Chem. 2019;67(7):2075.","journal-title":"J Agric Food Chem"},{"issue":"2\u20133","key":"6487_CR30","doi-asserted-by":"publisher","first-page":"270","DOI":"10.1016\/S0167-4889(00)00033-1","volume":"1496","author":"C-R Jan","year":"2000","unstructured":"Jan C-R, Lin M-C, Chou K-J, Huang J-K. Novel effects of gossypol, a chemical contraceptive in man: mobilization of internal Ca2+ and activation of external Ca2+ entry in intact cells. Biochimica et Biophysica Acta (BBA)-Mol Cell Res. 2000;1496(2\u20133):270\u20136.","journal-title":"Biochimica et Biophysica Acta (BBA)-Mol Cell Res"},{"issue":"7","key":"6487_CR31","doi-asserted-by":"publisher","first-page":"851","DOI":"10.1016\/S0041-0101(01)00194-5","volume":"40","author":"J-S Cheng","year":"2002","unstructured":"Cheng J-S, Liu C-P, Lo Y-K, Chou K-J, Lin M-C, Su W, Law Y-P, Wang J-L, Chen W-C, Jan C-R. Gossypol, a component in cottonseed, induced increases in cytosolic Ca2+ levels in Chang liver cells. Toxicon. 2002;40(7):851\u20136.","journal-title":"Toxicon"},{"key":"6487_CR32","first-page":"117","volume":"46","author":"J-S Cheng","year":"2003","unstructured":"Cheng J-S, Lo Y-K, Yeh J-H, Cheng H-H, Liu C-P, Chen W-C, Jan C-R. Effect of gossypol on intracellular Ca2+ regulation in human hepatoma cells. Chin J Phys. 2003;46:117\u201322.","journal-title":"Chin J Phys"},{"issue":"9","key":"6487_CR33","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1016\/j.tcb.2014.03.007","volume":"24","author":"M Sotomayor","year":"2014","unstructured":"Sotomayor M, Gaudet R, Corey DP. Sorting out a promiscuous superfamily: towards cadherin connectomics. Trends Cell Biol. 2014;24(9):524\u201336.","journal-title":"Trends Cell Biol"},{"issue":"1","key":"6487_CR34","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/j.neuroscience.2007.12.046","volume":"158","author":"C Sager","year":"2009","unstructured":"Sager C, Tapken D, Kott S, Hollmann M. Functional modulation of AMPA receptors by transmembrane AMPA receptor regulatory proteins. Neuroscience. 2009;158(1):45\u201354.","journal-title":"Neuroscience"},{"key":"6487_CR35","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.cimid.2018.11.005","volume":"62","author":"DB Moretti","year":"2019","unstructured":"Moretti DB, Jimenez CR, Trinca HM, Machado-Neto R, Louvandini H. Effect of maternal cottonseed feed on the immune and antioxidant status of Santa Ines lambs. Comp Immunol Microbiol Infect Dis. 2019;62:58\u201363.","journal-title":"Comp Immunol Microbiol Infect Dis"},{"issue":"1","key":"6487_CR36","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1017\/S0021859600020451","volume":"72","author":"JJ Robinson","year":"1969","unstructured":"Robinson JJ, Foster WH, Forbes TJ. The estimation of the milk yield of a ewe from body weight data on the suckling lamb. J Agric Sci. 1969;72(1):103\u20137.","journal-title":"J Agric Sci"},{"issue":"4","key":"6487_CR37","doi-asserted-by":"publisher","first-page":"R36","DOI":"10.1186\/gb-2013-14-4-r36","volume":"14","author":"D Kim","year":"2013","unstructured":"Kim D, Pertea G, Trapnell C, Pimentel H, Kelley R, Salzberg S. TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions. Genome Biol. 2013;14(4):R36.","journal-title":"Genome Biol"},{"issue":"2","key":"6487_CR38","doi-asserted-by":"publisher","first-page":"166","DOI":"10.1093\/bioinformatics\/btu638","volume":"31","author":"S Anders","year":"2015","unstructured":"Anders S, Pyl PT, Huber W. HTSeq-a Python framework to work with high-throughput sequencing data. Bioinformatics. 2015;31(2):166\u20139.","journal-title":"Bioinformatics"},{"issue":"1","key":"6487_CR39","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1093\/bioinformatics\/btp616","volume":"26","author":"MD Robinson","year":"2010","unstructured":"Robinson MD, McCarthy DJ, Smyth GK. edgeR: a bioconductor package for differential expression analysis of digital gene expression data. Bioinformatics. 2010;26(1):139\u201340.","journal-title":"Bioinformatics"},{"issue":"8","key":"6487_CR40","doi-asserted-by":"publisher","first-page":"4174","DOI":"10.3168\/jds.2010-4137","volume":"94","author":"B Zhang","year":"2011","unstructured":"Zhang B, Pe\u00f1agaricano F, Driver A, Chen H, Khatib H. Differential expression of heat shock protein genes and their splice variants in bovine preimplantation embryos. J Dairy Sci. 2011;94(8):4174\u201382.","journal-title":"J Dairy Sci"},{"issue":"4","key":"6487_CR41","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2-DDCT method. Methods. 2001;25(4):402\u20138.","journal-title":"Methods"},{"issue":"1","key":"6487_CR42","doi-asserted-by":"publisher","first-page":"e1001057","DOI":"10.1371\/journal.pcbi.1001057","volume":"7","author":"P Langfelder","year":"2011","unstructured":"Langfelder P, Luo R, Oldham MC, Horvath S. Is my network module preserved and reproducible? PLoS Comput Biol. 2011;7(1):e1001057.","journal-title":"PLoS Comput Biol"},{"issue":"1","key":"6487_CR43","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1186\/1471-2105-9-559","volume":"9","author":"P Langfelder","year":"2008","unstructured":"Langfelder P, Horvath S. WGCNA: an R package for weighted correlation network analysis. BMC Bioinformatics. 2008;9(1):559.","journal-title":"BMC Bioinformatics"}],"container-title":["BMC Genomics"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12864-020-6487-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s12864-020-6487-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12864-020-6487-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T19:07:14Z","timestamp":1610737634000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcgenomics.biomedcentral.com\/articles\/10.1186\/s12864-020-6487-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,17]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["6487"],"URL":"https:\/\/doi.org\/10.1186\/s12864-020-6487-2","relation":{},"ISSN":["1471-2164"],"issn-type":[{"value":"1471-2164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,17]]},"assertion":[{"value":"5 September 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 January 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 January 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"All the animal procedures were approved by the Committee on Animal Research and Ethics (008\/2015) of the Center of Nuclear Energy in Agriculture, University of S\u00e3o Paulo, Brazil.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The corresponding author, Francisco Pe\u00f1agaricano, is a member of the editorial board for the BMC Genomics journal.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"59"}}