{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T22:05:32Z","timestamp":1784066732047,"version":"3.55.0"},"reference-count":92,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,4,15]],"date-time":"2020-04-15T00:00:00Z","timestamp":1586908800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,4,15]],"date-time":"2020-04-15T00:00:00Z","timestamp":1586908800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/J004669\/1"],"award-info":[{"award-number":["BB\/J004669\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/P016774\/1"],"award-info":[{"award-number":["BB\/P016774\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BBS\/E\/T\/000PR9817"],"award-info":[{"award-number":["BBS\/E\/T\/000PR9817"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Genomics"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The Weddell Seal (\n                      <jats:italic>Leptonychotes weddelli<\/jats:italic>\n                      ) represents a remarkable example of adaptation to diving among marine mammals. This species is capable of diving &gt;\u2009900\u2009m deep and remaining underwater for more than 60\u2009min. A number of key physiological specializations have been identified, including the low levels of aerobic, lipid-based metabolism under hypoxia, significant increase in oxygen storage in blood and muscle; high blood volume and extreme cardiovascular control. These adaptations have been linked to increased abundance of key proteins, suggesting an important, yet still understudied role for gene reprogramming.\n                    <\/jats:p>\n                    <jats:p>In this study, we investigate the possibility that post-transcriptional gene regulation by microRNAs (miRNAs) has contributed to the adaptive evolution of diving capacities in the Weddell Seal.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Using small RNA data across 4 tissues (brain, heart, muscle and plasma), in 3 biological replicates, we generate the first miRNA annotation in this species, consisting of 559 high confidence, manually curated miRNA loci. Evolutionary analyses of miRNA gain and loss highlight a high number of Weddell seal specific miRNAs.<\/jats:p>\n                    <jats:p>Four hundred sixteen miRNAs were differentially expressed (DE) among tissues, whereas 80 miRNAs were differentially expressed (DE) across all tissues between pups and adults and age differences for specific tissues were detected in 188 miRNAs. mRNA targets of these altered miRNAs identify possible protective mechanisms in individual tissues, particularly relevant to hypoxia tolerance, anti-apoptotic pathways, and nitric oxide signal transduction. Novel, lineage-specific miRNAs associated with developmental changes target genes with roles in angiogenesis and vasoregulatory signaling.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Altogether, we provide an overview of miRNA composition and evolution in the Weddell seal, and the first insights into their possible role in the specialization to diving.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12864-020-6675-0","type":"journal-article","created":{"date-parts":[[2020,4,15]],"date-time":"2020-04-15T07:03:17Z","timestamp":1586934197000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["microRNA profiling in the Weddell seal suggests novel regulatory mechanisms contributing to diving adaptation"],"prefix":"10.1186","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4893-2400","authenticated-orcid":false,"given":"Luca","family":"Penso-Dolfin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilfried","family":"Haerty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allyson","family":"Hindle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Di Palma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,4,15]]},"reference":[{"key":"6675_CR1","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.dsr2.2012.08.025","volume":"88","author":"K Heerah","year":"2013","unstructured":"Heerah K, Andrews-Goff V, Williams G, Sultan E, Hindell M, Patterson T, Charrassin J-B. Ecology of Weddell seals during winter: influence of environmental parameters on their foraging behaviour. Deep-Sea Res II Top Stud Oceanogr. 2013;88:23\u201333.","journal-title":"Deep-Sea Res II Top Stud Oceanogr."},{"issue":"6","key":"6675_CR2","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1038\/scientificamerican0687-100","volume":"256","author":"WM Zapol","year":"1987","unstructured":"Zapol WM. Diving adaptations of the Weddell seal. Sci Am. 1987;256(6):100\u20137.","journal-title":"Sci Am."},{"issue":"6","key":"6675_CR3","doi-asserted-by":"publisher","first-page":"514","DOI":"10.1002\/ar.20545","volume":"290","author":"MD Uhen","year":"2007","unstructured":"Uhen MD. Evolution of marine mammals: back to the sea after 300 million years. Anat Rec. 2007;290(6):514\u201322.","journal-title":"Anat Rec."},{"issue":"5","key":"6675_CR4","doi-asserted-by":"publisher","first-page":"806","DOI":"10.1242\/jeb.060681","volume":"215","author":"MA De Miranda","year":"2012","unstructured":"De Miranda MA, Schlater AE, Green TL, Kanatous SB. In the face of hypoxia: myoglobin increases in response to hypoxic conditions and lipid supplementation in cultured Weddell seal skeletal muscle cells. J Exp Biol. 2012;215(5):806\u201313.","journal-title":"J Exp Biol."},{"issue":"23","key":"6675_CR5","doi-asserted-by":"crossref","first-page":"3601","DOI":"10.1242\/jeb.205.23.3601","volume":"205","author":"S Kanatous","year":"2002","unstructured":"Kanatous S, Davis R, Watson R, Polasek L, Williams T, Mathieu-Costello O. Aerobic capacities in the skeletal muscles of Weddell seals: key to longer dive durations? J Exp Biol. 2002;205(23):3601\u20138.","journal-title":"J Exp Biol."},{"issue":"10","key":"6675_CR6","doi-asserted-by":"publisher","first-page":"1676","DOI":"10.1242\/jeb.040923","volume":"213","author":"SJ Trumble","year":"2010","unstructured":"Trumble SJ, Noren SR, Cornick LA, Hawke TJ, Kanatous SB. Age-related differences in skeletal muscle lipid profiles of Weddell seals: clues to developmental changes. J Exp Biol. 2010;213(10):1676\u201384.","journal-title":"J Exp Biol."},{"issue":"6138","key":"6675_CR7","doi-asserted-by":"publisher","first-page":"1234192","DOI":"10.1126\/science.1234192","volume":"340","author":"S Mirceta","year":"2013","unstructured":"Mirceta S, Signore AV, Burns JM, Cossins AR, Campbell KL, Berenbrink M. Evolution of mammalian diving capacity traced by myoglobin net surface charge. Science. 2013;340(6138):1234192.","journal-title":"Science."},{"issue":"20","key":"6675_CR8","doi-asserted-by":"publisher","first-page":"3325","DOI":"10.1242\/jeb.031252","volume":"214","author":"PJ Ponganis","year":"2011","unstructured":"Ponganis PJ, Meir JU, Williams CL. In pursuit of Irving and Scholander: a review of oxygen store management in seals and penguins. J Exp Biol. 2011;214(20):3325\u201339.","journal-title":"J Exp Biol."},{"issue":"6","key":"6675_CR9","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1038\/scientificamerican1263-92","volume":"209","author":"PF Scholander","year":"1963","unstructured":"Scholander PF. The master switch of life. Sci Am. 1963;209(6):92\u2013107.","journal-title":"Sci Am."},{"issue":"6","key":"6675_CR10","first-page":"R704","volume":"316","author":"AG Hindle","year":"2019","unstructured":"Hindle AG, Allen KN, Batten AJ, H\u00fcckst\u00e4dt LA, Turner-Maier J, Schulberg SA, Johnson J, Karlsson E, Lindblad-Toh K, Costa DP. Low guanylyl cyclase activity in Weddell seals: implications for peripheral vasoconstriction and perfusion of the brain during diving. Am J Phys Regul Integr Comp Phys. 2019;316(6):R704\u201315.","journal-title":"Am J Phys Regul Integr Comp Phys."},{"issue":"1","key":"6675_CR11","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/S0092-8674(03)00231-9","volume":"113","author":"J Brennecke","year":"2003","unstructured":"Brennecke J, Hipfner DR, Stark A, Russell RB, Cohen SM. Bantam encodes a developmentally regulated microRNA that controls cell proliferation and regulates the proapoptotic gene hid in Drosophila. Cell. 2003;113(1):25\u201336.","journal-title":"Cell."},{"issue":"7","key":"6675_CR12","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1016\/j.cub.2008.02.081","volume":"18","author":"N Bushati","year":"2008","unstructured":"Bushati N, Stark A, Brennecke J, Cohen SM. Temporal reciprocity of miRNAs and their targets during the maternal-to-zygotic transition in Drosophila. Curr Biol. 2008;18(7):501\u20136.","journal-title":"Curr Biol."},{"issue":"5","key":"6675_CR13","doi-asserted-by":"publisher","first-page":"843","DOI":"10.1016\/0092-8674(93)90529-Y","volume":"75","author":"RC Lee","year":"1993","unstructured":"Lee RC, Feinbaum RL, Ambros V. The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell. 1993;75(5):843\u201354.","journal-title":"Cell."},{"issue":"9","key":"6675_CR14","doi-asserted-by":"publisher","first-page":"790","DOI":"10.1016\/S0960-9822(03)00250-1","volume":"13","author":"P Xu","year":"2003","unstructured":"Xu P, Vernooy SY, Guo M, Hay BA. The Drosophila microRNA Mir-14 suppresses cell death and is required for normal fat metabolism. Curr Biol. 2003;13(9):790\u20135.","journal-title":"Curr Biol."},{"issue":"2","key":"6675_CR15","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1016\/S0092-8674(04)00045-5","volume":"116","author":"DP Bartel","year":"2004","unstructured":"Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004;116(2):281\u201397.","journal-title":"Cell."},{"issue":"4","key":"6675_CR16","doi-asserted-by":"publisher","first-page":"642","DOI":"10.1016\/j.cell.2009.01.035","volume":"136","author":"RW Carthew","year":"2009","unstructured":"Carthew RW, Sontheimer EJ. Origins and mechanisms of miRNAs and siRNAs. Cell. 2009;136(4):642\u201355.","journal-title":"Cell."},{"issue":"2","key":"6675_CR17","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/j.gde.2007.02.004","volume":"17","author":"R Niwa","year":"2007","unstructured":"Niwa R, Slack FJ. The evolution of animal microRNA function. Curr Opin Genet Dev. 2007;17(2):145\u201350.","journal-title":"Curr Opin Genet Dev."},{"issue":"5755","key":"6675_CR18","doi-asserted-by":"publisher","first-page":"1817","DOI":"10.1126\/science.1121158","volume":"310","author":"KK-H Farh","year":"2005","unstructured":"Farh KK-H, Grimson A, Jan C, Lewis BP, Johnston WK, Lim LP, Burge CB, Bartel DP. The widespread impact of mammalian MicroRNAs on mRNA repression and evolution. Science. 2005;310(5755):1817\u201321.","journal-title":"Science."},{"issue":"3","key":"6675_CR19","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1007\/s00335-005-0066-3","volume":"17","author":"C Sevignani","year":"2006","unstructured":"Sevignani C, Calin GA, Siracusa LD, Croce CM. Mammalian microRNAs: a small world for fine-tuning gene expression. Mamm Genome. 2006;17(3):189\u2013202.","journal-title":"Mamm Genome."},{"key":"6675_CR20","doi-asserted-by":"publisher","first-page":"e3959","DOI":"10.7717\/peerj.3959","volume":"5","author":"J Guan","year":"2017","unstructured":"Guan J, Long K, Ma J, Zhang J, He D, Jin L, Tang Q, Jiang A, Wang X, Hu Y. Comparative analysis of the microRNA transcriptome between yak and cattle provides insight into high-altitude adaptation. PeerJ. 2017;5:e3959.","journal-title":"PeerJ."},{"issue":"2","key":"6675_CR21","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1093\/jmcb\/mjx053","volume":"10","author":"KK Biggar","year":"2018","unstructured":"Biggar KK, Storey KB. Functional impact of microRNA regulation in models of extreme stress adaptation. J Mol Cell Biol. 2018;10(2):93\u2013101.","journal-title":"J Mol Cell Biol."},{"key":"6675_CR22","doi-asserted-by":"publisher","first-page":"230","DOI":"10.3389\/fgene.2018.00230","volume":"9","author":"CL Riggs","year":"2018","unstructured":"Riggs CL, Summers A, Warren DE, Nilsson GE, Lefevre S, Dowd W, Milton S, Podrabsky JE. Small non-coding RNA expression and vertebrate anoxia tolerance. Front Genet. 2018;9:230.","journal-title":"Front Genet."},{"issue":"6","key":"6675_CR23","doi-asserted-by":"publisher","first-page":"586","DOI":"10.1016\/j.bbagrm.2018.04.007","volume":"1861","author":"H Hadj-Moussa","year":"2018","unstructured":"Hadj-Moussa H, Logan SM, Seibel BA, Storey KB. Potential role for microRNA in regulating hypoxia-induced metabolic suppression in jumbo squids. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms. 2018;1861(6):586\u201393.","journal-title":"Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms"},{"issue":"4","key":"6675_CR24","first-page":"358","volume":"8","author":"PJ Lyons","year":"2013","unstructured":"Lyons PJ, Lang-Ouellette D. CryomiRs: towards the identification of a cold-associated family of microRNAs. Comp Biochem Physiol Part D: Genomics Proteomics. 2013;8(4):358\u201364.","journal-title":"Comp Biochem Physiol Part D: Genomics Proteomics."},{"key":"6675_CR25","doi-asserted-by":"publisher","first-page":"8866","DOI":"10.1038\/srep08866","volume":"5","author":"C Nehammer","year":"2015","unstructured":"Nehammer C, Podolska A, Mackowiak SD, Kagias K, Pocock R. Specific microRNAs regulate heat stress responses in Caenorhabditis elegans. Sci Rep. 2015;5:8866.","journal-title":"Sci Rep."},{"issue":"Database issue","key":"6675_CR26","doi-asserted-by":"publisher","first-page":"D68","DOI":"10.1093\/nar\/gkt1181","volume":"42","author":"A Kozomara","year":"2014","unstructured":"Kozomara A, Griffiths-Jones S. miRBase: annotating high confidence microRNAs using deep sequencing data. Nucleic Acids Res. 2014;42(Database issue):D68\u201373 https:\/\/doi.org\/10.1093\/nar\/gkt1181.","journal-title":"Nucleic Acids Res."},{"issue":"16","key":"6675_CR27","doi-asserted-by":"publisher","first-page":"2446","DOI":"10.1093\/bioinformatics\/btx210","volume":"33","author":"C Paicu","year":"2017","unstructured":"Paicu C, Mohorianu I, Stocks M, Xu P, Coince A, Billmeier M, Dalmay T, Moulton V, Moxon S. miRCat2: accurate prediction of plant and animal microRNAs from next-generation sequencing datasets. Bioinformatics. 2017;33(16):2446\u201354.","journal-title":"Bioinformatics."},{"issue":"1","key":"6675_CR28","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1093\/nar\/gkr688","volume":"40","author":"MR Friedl\u00e4nder","year":"2011","unstructured":"Friedl\u00e4nder MR, Mackowiak SD, Li N, Chen W, Rajewsky N. miRDeep2 accurately identifies known and hundreds of novel microRNA genes in seven animal clades. Nucleic Acids Res. 2011;40(1):37\u201352.","journal-title":"Nucleic Acids Res."},{"issue":"11","key":"6675_CR29","doi-asserted-by":"publisher","first-page":"1944","DOI":"10.1373\/clinchem.2009.125310","volume":"55","author":"X Ji","year":"2009","unstructured":"Ji X, Takahashi R, Hiura Y, Hirokawa G, Fukushima Y, Iwai N. Plasma miR-208 as a biomarker of myocardial injury. Clin Chem. 2009;55(11):1944\u20139.","journal-title":"Clin Chem."},{"issue":"7","key":"6675_CR30","doi-asserted-by":"publisher","first-page":"135","DOI":"10.21037\/atm.2016.03.40","volume":"4","author":"Y Xin","year":"2016","unstructured":"Xin Y, Yang C, Han Z. Circulating miR-499 as a potential biomarker for acute myocardial infarction. Ann Translational Med. 2016;4(7):135.","journal-title":"Ann Translational Med."},{"issue":"2","key":"6675_CR31","doi-asserted-by":"publisher","first-page":"405","DOI":"10.3390\/ijms19020405","volume":"19","author":"S Zhang","year":"2018","unstructured":"Zhang S, Chen N. Regulatory role of MicroRNAs in muscle atrophy during exercise intervention. Int J Mol Sci. 2018;19(2):405.","journal-title":"Int J Mol Sci."},{"issue":"1","key":"6675_CR32","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1101\/gr.140269.112","volume":"23","author":"J Meunier","year":"2013","unstructured":"Meunier J, Lemoine F, Soumillon M, Liechti A, Weier M, Guschanski K, Hu H, Khaitovich P, Kaessmann H. Birth and expression evolution of mammalian microRNA genes. Genome Res. 2013;23(1):34\u201345.","journal-title":"Genome Res."},{"issue":"1","key":"6675_CR33","doi-asserted-by":"publisher","first-page":"17050","DOI":"10.1038\/s41598-018-34243-8","volume":"8","author":"L Penso-Dolfin","year":"2018","unstructured":"Penso-Dolfin L, Moxon S, Haerty W, Di Palma F. The evolutionary dynamics of microRNAs in domestic mammals. Sci Rep. 2018;8(1):17050.","journal-title":"Sci Rep."},{"issue":"3","key":"6675_CR34","doi-asserted-by":"publisher","first-page":"e91172","DOI":"10.1371\/journal.pone.0091172","volume":"9","author":"MP Hoeppner","year":"2014","unstructured":"Hoeppner MP, Lundquist A, Pirun M, Meadows JR, Zamani N, Johnson J, Sundstr\u00f6m G, Cook A, FitzGerald MG, Swofford R. An improved canine genome and a comprehensive catalogue of coding genes and non-coding transcripts. PLoS One. 2014;9(3):e91172.","journal-title":"PLoS One."},{"issue":"2","key":"6675_CR35","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1002\/bdrc.20072","volume":"78","author":"CT Lee","year":"2006","unstructured":"Lee CT, Risom T, Strauss WM. MicroRNAs in mammalian development. Birth Defects Res C Embryo Today. 2006;78(2):129\u201339.","journal-title":"Birth Defects Res C Embryo Today."},{"issue":"2","key":"6675_CR36","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1002\/bdrc.20070","volume":"78","author":"L Song","year":"2006","unstructured":"Song L, Tuan RS. MicroRNAs and cell differentiation in mammalian development. Birth Defects Res C Embryo Today. 2006;78(2):140\u20139.","journal-title":"Birth Defects Res C Embryo Today."},{"issue":"9","key":"6675_CR37","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1242\/jeb.202.9.1091","volume":"202","author":"RW Davis","year":"1999","unstructured":"Davis RW, Kanatous SB. Convective oxygen transport and tissue oxygen consumption in Weddell seals during aerobic dives. J Exp Biol. 1999;202(9):1091\u2013113.","journal-title":"J Exp Biol."},{"issue":"4","key":"6675_CR38","doi-asserted-by":"publisher","first-page":"1148","DOI":"10.1152\/jappl.1995.79.4.1148","volume":"79","author":"GP Guyton","year":"1995","unstructured":"Guyton GP, Stanek KS, Schneider RC, Hochachka PW, Hurford WE, Zapol DG, Liggins GC, Zapol WM. Myoglobin saturation in free-diving Weddell seals. J Appl Physiol. 1995;79(4):1148\u201355.","journal-title":"J Appl Physiol."},{"issue":"24","key":"6675_CR39","doi-asserted-by":"crossref","first-page":"35607","DOI":"10.18632\/oncotarget.9597","volume":"7","author":"Y Li","year":"2016","unstructured":"Li Y, Lu J, Bao X, Wang X, Wu J, Li X, Hong W. MiR-499-5p protects cardiomyocytes against ischaemic injury via anti-apoptosis by targeting PDCD4. Oncotarget. 2016;7(24):35607.","journal-title":"Oncotarget."},{"issue":"4","key":"6675_CR40","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1042\/CS20180465","volume":"133","author":"J Tang","year":"2019","unstructured":"Tang J, Zhuang S. Histone acetylation and DNA methylation in ischemia\/reperfusion injury. Clin Sci. 2019;133(4):597\u2013609.","journal-title":"Clin Sci."},{"key":"6675_CR41","doi-asserted-by":"publisher","first-page":"6055","DOI":"10.1038\/ncomms7055","volume":"6","author":"TM Williams","year":"2015","unstructured":"Williams TM, Fuiman LA, Kendall T, Berry P, Richter B, Noren SR, Thometz N, Shattock MJ, Farrell E, Stamper AM. Exercise at depth alters bradycardia and incidence of cardiac anomalies in deep-diving marine mammals. Nat Commun. 2015;6:6055.","journal-title":"Nat Commun."},{"issue":"6","key":"6675_CR42","doi-asserted-by":"publisher","first-page":"2364","DOI":"10.1159\/000452506","volume":"39","author":"J Zhu","year":"2016","unstructured":"Zhu J, Yao K, Wang Q, Guo J, Shi H, Ma L, Liu H, Gao W, Zou Y, Ge J. Ischemic postconditioning-regulated miR-499 protects the rat heart against ischemia\/reperfusion injury by inhibiting apoptosis through PDCD4. Cell Physiol Biochem. 2016;39(6):2364\u201380.","journal-title":"Cell Physiol Biochem."},{"issue":"2","key":"6675_CR43","doi-asserted-by":"publisher","first-page":"864","DOI":"10.1159\/000490078","volume":"47","author":"M Jiang","year":"2018","unstructured":"Jiang M, Wang H, Jin M, Yang X, Ji H, Jiang Y, Zhang H, Wu F, Wu G, Lai X. Exosomes from MiR-30d-5p-ADSCs reverse acute ischemic stroke-induced, autophagy-mediated brain injury by promoting M2 microglial\/macrophage polarization. Cell Physiol Biochem. 2018;47(2):864\u201378.","journal-title":"Cell Physiol Biochem."},{"issue":"2","key":"6675_CR44","doi-asserted-by":"publisher","first-page":"272","DOI":"10.1093\/cvr\/cvt172","volume":"100","author":"Y Sun","year":"2013","unstructured":"Sun Y, Chen D, Cao L, Zhang R, Zhou J, Chen H, Li Y, Li M, Cao J, Wang Z. MiR-490-3p modulates the proliferation of vascular smooth muscle cells induced by ox-LDL through targeting PAPP-A. Cardiovasc Res. 2013;100(2):272\u20139.","journal-title":"Cardiovasc Res."},{"issue":"4","key":"6675_CR45","doi-asserted-by":"publisher","first-page":"739","DOI":"10.1016\/j.neuron.2010.09.029","volume":"68","author":"WT Birdsong","year":"2010","unstructured":"Birdsong WT, Fierro L, Williams FG, Spelta V, Naves LA, Knowles M, Marsh-Haffner J, Adelman JP, Almers W, Elde RP. Sensing muscle ischemia: coincident detection of acid and ATP via interplay of two ion channels. Neuron. 2010;68(4):739\u201349.","journal-title":"Neuron."},{"issue":"1_supplement","key":"6675_CR46","first-page":"861","volume":"32","author":"K Allen","year":"2018","unstructured":"Allen K, Hindle A, V\u00e1zquez-Medina JP, Lawler JM, Mellish J-AE, Horning M. Age-and Muscle-Specific Oxidative Stress Management Strategies in a Long-Lived Diver, the Weddell Seal. FASEB J. 2018;32(1_supplement):861\u20135.","journal-title":"FASEB J"},{"issue":"3\u20134","key":"6675_CR47","first-page":"198","volume":"142","author":"JP V\u00e1zquez-Medina","year":"2006","unstructured":"V\u00e1zquez-Medina JP, Zenteno-Sav\u00edn T, Elsner R. Antioxidant enzymes in ringed seal tissues: potential protection against dive-associated ischemia\/reperfusion. Comp Biochem Physiol C: Toxicol Pharmacol. 2006;142(3\u20134):198\u2013204.","journal-title":"Comp Biochem Physiol C: Toxicol Pharmacol."},{"issue":"4","key":"6675_CR48","doi-asserted-by":"publisher","first-page":"356","DOI":"10.1530\/acta.0.1290356","volume":"129","author":"GC Liggins","year":"1993","unstructured":"Liggins GC, France JT, Schneider RC, Knox BS, Zapol WM. Concentrations, metabolic clearance rates, production rates and plasma binding of cortisol in Antarctic phocid seals. Eur J Endocrinol. 1993;129(4):356\u20139.","journal-title":"Eur J Endocrinol."},{"issue":"5","key":"6675_CR49","doi-asserted-by":"publisher","first-page":"737","DOI":"10.1139\/z99-022","volume":"77","author":"JM Burns","year":"1999","unstructured":"Burns JM. The development of diving behavior in juvenile Weddell seals: pushing physiological limits in order to survive. Can J Zool. 1999;77(5):737\u201347.","journal-title":"Can J Zool."},{"issue":"2","key":"6675_CR50","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1007\/BF00689915","volume":"151","author":"G Kooyman","year":"1983","unstructured":"Kooyman G, Castellini M, Davis R, Maue R. Aerobic diving limits of immature Weddell seals. J Comp Physiol B: Biochemical Systemic Environ Physiology. 1983;151(2):171\u20134.","journal-title":"J Comp Physiol B: Biochemical Systemic Environ Physiology."},{"issue":"4","key":"6675_CR51","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1007\/BF00691568","volume":"138","author":"G Kooyman","year":"1980","unstructured":"Kooyman G, Wahrenbrock E, Castellini M, Davis R, Sinnett E. Aerobic and anaerobic metabolism during voluntary diving in Weddell seals: evidence of preferred pathways from blood chemsitry and behavior. J Comp Physiol B: Biochemical Systemic Environ Physiology. 1980;138(4):335\u201346.","journal-title":"J Comp Physiol B: Biochemical Systemic Environ Physiology."},{"issue":"5","key":"6675_CR52","doi-asserted-by":"publisher","first-page":"732","DOI":"10.1086\/physzool.66.5.30163821","volume":"66","author":"PJ Ponganis","year":"1993","unstructured":"Ponganis PJ, Kooyman GL, Castellini MA. Determinants of the aerobic dive limit of Weddell seals: analysis of diving metabolic rates, postdive end tidal PO2's, and blood and muscle oxygen stores. Physiol Zool. 1993;66(5):732\u201349.","journal-title":"Physiol Zool."},{"key":"6675_CR53","first-page":"417","volume-title":"Proceedings of the 4th CPB Meeting in Africa: MARA","author":"J Burns","year":"2008","unstructured":"Burns J, Hammill M. Does iron availability limit oxygen store development in seal pups. In: Proceedings of the 4th CPB Meeting in Africa: MARA; 2008. p. 417\u201328."},{"issue":"10","key":"6675_CR54","doi-asserted-by":"publisher","first-page":"1793","DOI":"10.1242\/jeb.082099","volume":"216","author":"SJ Geiseler","year":"2013","unstructured":"Geiseler SJ, Blix AS, Burns JM, Folkow LP. Rapid postnatal development of myoglobin from large liver iron stores in hooded seals. J Exp Biol. 2013;216(10):1793\u20138.","journal-title":"J Exp Biol."},{"issue":"2","key":"6675_CR55","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1053\/beha.2002.0207","volume":"15","author":"C Camaschella","year":"2002","unstructured":"Camaschella C, Roetto A, De Gobbi M. Genetic haemochromatosis: genes and mutations associated with iron loading. Best Pract Res Clin Haematol. 2002;15(2):261\u201376.","journal-title":"Best Pract Res Clin Haematol."},{"issue":"2","key":"6675_CR56","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1038\/84859","volume":"27","author":"T LaVaute","year":"2001","unstructured":"LaVaute T, Smith S, Cooperman S, Iwai K, Land W, Meyron-Holtz E, Drake SK, Miller G, Abu-Asab M, Tsokos M. Targeted deletion of the gene encoding iron regulatory protein-2 causes misregulation of iron metabolism and neurodegenerative disease in mice. Nat Genet. 2001;27(2):209.","journal-title":"Nat Genet."},{"issue":"5","key":"6675_CR57","doi-asserted-by":"publisher","first-page":"757","DOI":"10.1007\/s00360-010-0448-z","volume":"180","author":"J Prewitt","year":"2010","unstructured":"Prewitt J, Freistroffer D, Schreer J, Hammill M, Burns J. Postnatal development of muscle biochemistry in nursing harbor seal (Phoca vitulina) pups: limitations to diving behavior? J Comp Physiol B. 2010;180(5):757\u201366.","journal-title":"J Comp Physiol B."},{"issue":"5","key":"6675_CR58","doi-asserted-by":"publisher","first-page":"740","DOI":"10.1242\/jeb.037929","volume":"213","author":"J Burns","year":"2010","unstructured":"Burns J, Skomp N, Bishop N, Lestyk K, Hammill M. Development of aerobic and anaerobic metabolism in cardiac and skeletal muscles from harp and hooded seals. J Exp Biol. 2010;213(5):740\u20138.","journal-title":"J Exp Biol."},{"issue":"16","key":"6675_CR59","doi-asserted-by":"publisher","first-page":"2559","DOI":"10.1242\/jeb.018119","volume":"211","author":"S Kanatous","year":"2008","unstructured":"Kanatous S, Hawke T, Trumble S, Pearson L, Watson R, Garry D, Williams T, Davis R. The ontogeny of aerobic and diving capacity in the skeletal muscles of Weddell seals. J Exp Biol. 2008;211(16):2559\u201365.","journal-title":"J Exp Biol."},{"issue":"5","key":"6675_CR60","first-page":"R743","volume":"245","author":"R Davis","year":"1983","unstructured":"Davis R, Castellini M, Kooyman G, Maue R. Renal glomerular filtration rate and hepatic blood flow during voluntary diving in Weddell seals. Am J Phys Regul Integr Comp Phys. 1983;245(5):R743\u20138.","journal-title":"Am J Phys Regul Integr Comp Phys."},{"issue":"3","key":"6675_CR61","doi-asserted-by":"publisher","first-page":"424","DOI":"10.1152\/jappl.1980.49.3.424","volume":"49","author":"G Liggins","year":"1980","unstructured":"Liggins G, Qvist J, Hochachka P, Murphy B, Creasy R, Schneider R, Snider M, Zapol W. Fetal cardiovascular and metabolic responses to simulated diving in the Weddell seal. J Appl Physiol. 1980;49(3):424\u201330.","journal-title":"J Appl Physiol."},{"issue":"6","key":"6675_CR62","doi-asserted-by":"publisher","first-page":"e3000306","DOI":"10.1371\/journal.pbio.3000306","volume":"17","author":"JC McKnight","year":"2019","unstructured":"McKnight JC, Bennett KA, Bronkhorst M, Russell DJ, Balfour S, Milne R, Bivins M, Moss SE, Colier W, Hall AJ. Shining new light on mammalian diving physiology using wearable near-infrared spectroscopy. PLoS Biol. 2019;17(6):e3000306.","journal-title":"PLoS Biol."},{"issue":"5","key":"6675_CR63","doi-asserted-by":"publisher","first-page":"968","DOI":"10.1152\/jappl.1979.47.5.968","volume":"47","author":"WM Zapol","year":"1979","unstructured":"Zapol WM, Liggins G, Schneider RC, Qvist J, Snider MT, Creasy RK, Hochachka PW. Regional blood flow during simulated diving in the conscious Weddell seal. J Appl Physiol. 1979;47(5):968\u201373.","journal-title":"J Appl Physiol."},{"issue":"3","key":"6675_CR64","first-page":"H991","volume":"278","author":"CM Brophy","year":"2000","unstructured":"Brophy CM, Knoepp L, Xin J, Pollock JS. Functional expression of NOS 1 in vascular smooth muscle. Am J Phys Heart Circ Phys. 2000;278(3):H991\u20137.","journal-title":"Am J Phys Heart Circ Phys."},{"issue":"12","key":"6675_CR65","doi-asserted-by":"publisher","first-page":"1322","DOI":"10.1161\/01.RES.0000078171.52542.9E","volume":"92","author":"SA Khan","year":"2003","unstructured":"Khan SA, Skaf MW, Harrison RW, Lee K, Minhas KM, Kumar A, Fradley M, Shoukas AA, Berkowitz DE, Hare JM. Nitric oxide regulation of myocardial contractility and calcium cycling: independent impact of neuronal and endothelial nitric oxide synthases. Circ Res. 2003;92(12):1322\u20139.","journal-title":"Circ Res."},{"issue":"1","key":"6675_CR66","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/S0014-2999(01)01560-6","volume":"435","author":"BA Weissman","year":"2002","unstructured":"Weissman BA, Jones CL, Liu Q, Gross SS. Activation and inactivation of neuronal nitric oxide synthase: characterization of Ca2+\u2212dependent [125I] Calmodulin binding. Eur J Pharmacol. 2002;435(1):9\u201318.","journal-title":"Eur J Pharmacol."},{"issue":"11","key":"6675_CR67","doi-asserted-by":"publisher","first-page":"4141","DOI":"10.1172\/JCI42980","volume":"120","author":"G Ghosh","year":"2010","unstructured":"Ghosh G, Subramanian IV, Adhikari N, Zhang X, Joshi HP, Basi D, Chandrashekhar Y, Hall JL, Roy S, Zeng Y. Hypoxia-induced microRNA-424 expression in human endothelial cells regulates HIF-\u03b1 isoforms and promotes angiogenesis. J Clin Invest. 2010;120(11):4141\u201354.","journal-title":"J Clin Invest."},{"key":"6675_CR68","doi-asserted-by":"publisher","first-page":"e05005","DOI":"10.7554\/eLife.05005","volume":"4","author":"V Agarwal","year":"2015","unstructured":"Agarwal V, Bell GW, Nam J-W, Bartel DP. Predicting effective microRNA target sites in mammalian mRNAs. Elife. 2015;4:e05005.","journal-title":"Elife."},{"issue":"1","key":"6675_CR69","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1161\/CIRCRESAHA.113.300975","volume":"113","author":"SJ Matkovich","year":"2013","unstructured":"Matkovich SJ, Hu Y, Dorn GW. Regulation of cardiac microRNAs by cardiac microRNAs. Circ Res. 2013;113(1):62\u201371.","journal-title":"Circ Res."},{"issue":"6200","key":"6675_CR70","doi-asserted-by":"publisher","first-page":"1074","DOI":"10.1126\/science.1253714","volume":"345","author":"M Carneiro","year":"2014","unstructured":"Carneiro M, Rubin CJ, Di Palma F, Albert FW, Alfoldi J, Barrio AM, Pielberg G, Rafati N, Sayyab S, Turner-Maier J, et al. Rabbit genome analysis reveals a polygenic basis for phenotypic change during domestication. Science. 2014;345(6200):1074\u20139.","journal-title":"Science."},{"issue":"7518","key":"6675_CR71","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1038\/nature13726","volume":"513","author":"D Brawand","year":"2014","unstructured":"Brawand D, Wagner CE, Li YI, Malinsky M, Keller I, Fan S, Simakov O, Ng AY, Lim ZW, Bezault E, et al. The genomic substrate for adaptive radiation in African cichlid fish. Nature. 2014;513(7518):375\u201381.","journal-title":"Nature."},{"key":"6675_CR72","doi-asserted-by":"publisher","unstructured":"Mehta TK, Koch C, Nash W, Knaack SA, Sudhakar P, Olbei M, Bastkowski S, Penso-Dolfin L, Korcsmaros T, Haerty W, et al. Evolution of regulatory networks controlling adaptive traits in cichlids. bioRxiv:496034 https:\/\/doi.org\/10.1101\/496034.","DOI":"10.1101\/496034"},{"key":"6675_CR73","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1093\/gbe\/evq002","volume":"2","author":"CC Babbitt","year":"2010","unstructured":"Babbitt CC, Fedrigo O, Pfefferle AD, Boyle AP, Horvath JE, Furey TS, Wray GA. Both noncoding and protein-coding RNAs contribute to gene expression evolution in the primate brain. Genome Biol Evol. 2010;2:67\u201379.","journal-title":"Genome Biol Evol."},{"issue":"9","key":"6675_CR74","doi-asserted-by":"publisher","first-page":"693","DOI":"10.1038\/nrg1940","volume":"7","author":"P Khaitovich","year":"2006","unstructured":"Khaitovich P, Enard W, Lachmann M, Paabo S. Evolution of primate gene expression. Nat Rev Genet. 2006;7(9):693\u2013702.","journal-title":"Nat Rev Genet."},{"issue":"10","key":"6675_CR75","doi-asserted-by":"publisher","first-page":"e1002327","DOI":"10.1371\/journal.pgen.1002327","volume":"7","author":"HY Hu","year":"2011","unstructured":"Hu HY, Guo S, Xi J, Yan Z, Fu N, Zhang X, Menzel C, Liang H, Yang H, Zhao M, et al. MicroRNA expression and regulation in human, chimpanzee, and macaque brains. PLoS Genet. 2011;7(10):e1002327.","journal-title":"PLoS Genet."},{"issue":"1","key":"6675_CR76","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/j.gene.2004.11.034","volume":"345","author":"IK Jordan","year":"2005","unstructured":"Jordan IK, Marino-Ramirez L, Koonin EV. Evolutionary significance of gene expression divergence. Gene. 2005;345(1):119\u201326.","journal-title":"Gene."},{"key":"6675_CR77","unstructured":"FastQC [https:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/]."},{"issue":"1","key":"6675_CR78","doi-asserted-by":"publisher","first-page":"11.12. 11","DOI":"10.1002\/0471250953.bi1112s47","volume":"47","author":"AR Quinlan","year":"2014","unstructured":"Quinlan AR. BEDTools: the Swiss-army tool for genome feature analysis. Curr Protocols Bioinformatics. 2014;47(1):11.12. 11\u201334.","journal-title":"Curr Protocols Bioinformatics"},{"issue":"1","key":"6675_CR79","doi-asserted-by":"publisher","first-page":"12.12. 11","DOI":"10.1002\/0471250953.bi1202s26","volume":"26","author":"IL Hofacker","year":"2009","unstructured":"Hofacker IL. R NA Secondary Structure Analysis Using the Vienna RNA Package. Curr Protocols Bioinformatics. 2009;26(1):12.12. 11\u20136.","journal-title":"Curr Protocols Bioinformatics"},{"key":"6675_CR80","volume-title":"RStudio: Integrated Development for R. RStudio, Inc., Boston, MA","author":"RStudio Team","year":"2015","unstructured":"RStudio Team. RStudio: Integrated Development for R. RStudio, Inc., Boston, MA. 2015. URL http:\/\/www.rstudio.com\/."},{"issue":"12","key":"6675_CR81","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","volume":"15","author":"MI Love","year":"2014","unstructured":"Love MI, Huber W, Anders S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 2014;15(12):550.","journal-title":"Genome Biol."},{"issue":"1","key":"6675_CR82","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1023\/A:1010933404324","volume":"45","author":"L Breiman","year":"2001","unstructured":"Breiman L. Random forests. Mach Learn. 2001;45(1):5\u201332.","journal-title":"Mach Learn."},{"issue":"1","key":"6675_CR83","doi-asserted-by":"publisher","first-page":"328","DOI":"10.1186\/1471-2105-8-328","volume":"8","author":"R Diaz-Uriarte","year":"2007","unstructured":"Diaz-Uriarte R. GeneSrF and varSelRF: a web-based tool and R package for gene selection and classification using random forest. BMC Bioinformatics. 2007;8(1):328.","journal-title":"BMC Bioinformatics."},{"issue":"23","key":"6675_CR84","doi-asserted-by":"publisher","first-page":"3150","DOI":"10.1093\/bioinformatics\/bts565","volume":"28","author":"L Fu","year":"2012","unstructured":"Fu L, Niu B, Zhu Z, Wu S, Li W. CD-HIT: accelerated for clustering the next-generation sequencing data. Bioinformatics. 2012;28(23):3150\u20132.","journal-title":"Bioinformatics."},{"issue":"3","key":"6675_CR85","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","volume":"215","author":"SF Altschul","year":"1990","unstructured":"Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. J Mol Biol. 1990;215(3):403\u201310.","journal-title":"J Mol Biol."},{"key":"6675_CR86","volume-title":"PHYLIP (Phylogeny Inference Package) version 3.6. Distributed by the author","author":"J Felsenstein","year":"2005","unstructured":"Felsenstein J. PHYLIP (Phylogeny Inference Package) version 3.6. Distributed by the author. Seattle: Department of Genome Sciences, University of Washington; 2005."},{"issue":"1","key":"6675_CR87","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.2517-6161.1995.tb02031.x","volume":"57","author":"Y Benjamini","year":"1995","unstructured":"Benjamini Y, Hochberg Y. Controlling the false discovery rate: a practical and powerful approach to multiple testing. J R Stat Soc Ser B Methodol. 1995;57(1):289\u2013300.","journal-title":"J R Stat Soc Ser B Methodol."},{"issue":"1","key":"6675_CR88","doi-asserted-by":"publisher","first-page":"R1","DOI":"10.1186\/gb-2003-5-1-r1","volume":"5","author":"AJ Enright","year":"2003","unstructured":"Enright AJ, John B, Gaul U, Tuschl T, Sander C, Marks DS. MicroRNA targets in Drosophila. Genome Biol. 2003;5(1):R1.","journal-title":"Genome Biol."},{"issue":"10","key":"6675_CR89","doi-asserted-by":"publisher","first-page":"1278","DOI":"10.1038\/ng2135","volume":"39","author":"M Kertesz","year":"2007","unstructured":"Kertesz M, Iovino N, Unnerstall U, Gaul U, Segal E. The role of site accessibility in microRNA target recognition. Nat Genet. 2007;39(10):1278.","journal-title":"Nat Genet."},{"key":"6675_CR90","doi-asserted-by":"crossref","unstructured":"Raudvere U, Kolberg L, Kuzmin I, Arak T, Adler P, Peterson H, Vilo J. g: Profiler: a web server for functional enrichment analysis and conversions of gene lists (2019 update). Nucleic Acids Res. 2019.","DOI":"10.1093\/nar\/gkz369"},{"issue":"1","key":"6675_CR91","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/nar\/gkn923","volume":"37","author":"DW Huang","year":"2008","unstructured":"Huang DW, Sherman BT, Lempicki RA. Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists. Nucleic Acids Res. 2008;37(1):1\u201313.","journal-title":"Nucleic Acids Res."},{"issue":"1","key":"6675_CR92","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1038\/nprot.2008.211","volume":"4","author":"BT Sherman","year":"2009","unstructured":"Sherman BT, Lempicki RA. Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources. Nat Protoc. 2009;4(1):44\u201357.","journal-title":"Nat Protoc."}],"container-title":["BMC Genomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12864-020-6675-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1186\/s12864-020-6675-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1186\/s12864-020-6675-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,3]],"date-time":"2024-08-03T21:25:00Z","timestamp":1722720300000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcgenomics.biomedcentral.com\/articles\/10.1186\/s12864-020-6675-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,15]]},"references-count":92,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["6675"],"URL":"https:\/\/doi.org\/10.1186\/s12864-020-6675-0","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/851394","asserted-by":"object"},{"id-type":"doi","id":"10.21203\/rs.3.rs-15753\/v2","asserted-by":"object"},{"id-type":"doi","id":"10.21203\/rs.3.rs-15753\/v1","asserted-by":"object"}]},"ISSN":["1471-2164"],"issn-type":[{"value":"1471-2164","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,15]]},"assertion":[{"value":"5 December 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 March 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 April 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Samples were collected from wild Weddell seal adults and pups near McMurdo Station, Antarctica as part of a larger study [\n                      \n                      ]. Handling of <i>L. weddellii<\/i> individuals and sample export was conducted under appropriate scientific authorizations (National Marine Fisheries Service #19439, #18662, ACA #2016\u2013005) and approved by the Massachusetts General Hospital IACUC. No further permissions were required to study wild Weddell seals. Consent to participate is not applicable, since our study does not involve human individuals, or any animal owned by an institution, individual or farm.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"303"}}