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As a typical cell-rupture feeder,\n                      <jats:italic>E. onukii<\/jats:italic>\n                      secretes proteases while using its stylet to probe the tender shoots of tea plants (\n                      <jats:italic>Camellia sinensis<\/jats:italic>\n                      ). This study identified and analyzed proteases expressed specifically in the salivary gland (SG) and gut of\n                      <jats:italic>E. onukii<\/jats:italic>\n                      through enzymatic activity assays complemented with an integrated analysis of transcriptomic and proteomic data.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In total, 129 contigs representing seven types of putative proteases were identified. Transcript abundance of digestive proteases and enzymatic activity assays showed that cathepsin B-like protease, cathepsin L-like protease, and serine proteases (trypsin- and chymotrypsin-like protease) were highly abundant in the gut but moderately abundant in the SG. The abundance pattern of digestive proteases in the SG and gut of\n                      <jats:italic>E. onukii<\/jats:italic>\n                      differed from that of other hemipterans, including\n                      <jats:italic>Nilaparvata lugens<\/jats:italic>\n                      ,\n                      <jats:italic>Laodelphax striatellus<\/jats:italic>\n                      ,\n                      <jats:italic>Acyrthosiphum pisum<\/jats:italic>\n                      ,\n                      <jats:italic>Halyomorpha halys<\/jats:italic>\n                      and\n                      <jats:italic>Nephotettix cincticeps<\/jats:italic>\n                      . Phylogenetic analysis showed that aminopeptidase N-like proteins and serine proteases abundant in the SG or gut of hemipterans formed two distinct clusters.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Altogether, this study provides insightful information on the digestive system of\n                      <jats:italic>E. onukii<\/jats:italic>\n                      . Compared to five other hemipteran species, we observed different patterns of proteases abundant in the SG and gut of\n                      <jats:italic>E. onukii<\/jats:italic>\n                      . These results will be beneficial in understanding the interaction between tea plants and\n                      <jats:italic>E. onukii<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12864-021-07578-2","type":"journal-article","created":{"date-parts":[[2021,4,15]],"date-time":"2021-04-15T06:18:21Z","timestamp":1618467501000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Transcriptomic and proteomic analysis of putative digestive proteases in the salivary gland and gut of Empoasca (Matsumurasca) onukii Matsuda"],"prefix":"10.1186","volume":"22","author":[{"given":"Ensi","family":"Shao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujuan","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaomin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yichen","family":"Liao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufei","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sijun","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiong","family":"Guan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhipeng","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,4,15]]},"reference":[{"issue":"9","key":"7578_CR1","doi-asserted-by":"publisher","first-page":"1235","DOI":"10.1016\/j.jinsphys.2012.06.008","volume":"58","author":"S Jin","year":"2012","unstructured":"Jin S, Chen ZM, Backus EA, Sun XL, Xiao B. 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