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Our previous study showed that an approved drug pyronaridine (PND) is highly effective against CE, both <jats:italic>in vitro<\/jats:italic> and in an animal model. To identify possible target genes, transcriptome analysis was performed with <jats:italic>E. granulosus sensu stricto<\/jats:italic> protoscoleces treated with PND.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>A total of 1,321 genes were differentially expressed in protoscoleces treated with PND, including 541 upregulated and 780 downregulated genes. Gene ontology and KEGG analyses revealed that the spliceosome, mitogen-activated protein kinase (MAPK) pathway and ATP-binding cassette (ABC) transporters were the top three enriched pathways. Western blot analysis showed that PND treatment resulted in a dose-dependent increase in protein expression levels of <jats:italic>Eg<\/jats:italic>MKK1 (MKK3\/6-like) and <jats:italic>Eg<\/jats:italic>MKK2 (MEK1\/2-like), two members of MAPK cascades. Interestingly, several heat shock protein (HSP) genes were greatly downregulated including stress-inducible HSPs and their constitutive cognates, and some of them belong to <jats:italic>Echinococcus<\/jats:italic>-specific expansion of HSP70.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>PND has a great impact on the spliceosome, MAPK pathway and ABC transporters, which may underline the mechanisms by which PND kills <jats:italic>E. granulosus<\/jats:italic> protoscoleces. In addition, PND downregulates HSPs expression, suggesting a close relationship between the drug and HSPs.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12864-021-07875-w","type":"journal-article","created":{"date-parts":[[2021,7,13]],"date-time":"2021-07-13T09:04:26Z","timestamp":1626167066000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Transcriptome analysis uncovers the key pathways and candidate genes related to the treatment of Echinococcus granulosus protoscoleces with the repurposed drug pyronaridine"],"prefix":"10.1186","volume":"22","author":[{"given":"Yingfang","family":"Yu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weisi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjing","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueting","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Duan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shizhu","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiumin","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liping","family":"Duan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,7,13]]},"reference":[{"issue":"3","key":"7875_CR1","doi-asserted-by":"publisher","first-page":"518","DOI":"10.1128\/JCM.02420-15","volume":"54","author":"NI Agudelo Higuita","year":"2016","unstructured":"Agudelo Higuita NI, Brunetti E, McCloskey C. 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