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Approximately 80% of patients initially diagnosed with locally advanced or metastatic disease survive only 4\u201311\u2009months after diagnosis. Tremendous efforts have been made toward understanding the biology of PDAC.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>In this study, we first utilized next-generation sequencing technique and existing microarray datasets to identify significant differentially expressed genes between PDAC and non-tumor adjacent tissue. By comparing top significant survival genes in PDAC Gene Expression Profiling Interactive Analysis database and PDAC transcriptome data from patients, our integrated analysis discovered five potential central genes (i.e., <jats:italic>MYEOV<\/jats:italic>, <jats:italic>KCNN4<\/jats:italic>, <jats:italic>FAM83A<\/jats:italic>, <jats:italic>S100A16<\/jats:italic>, and <jats:italic>DDX60L<\/jats:italic>). Subsequently, we analyzed the cellular functions of the potential novel oncogenes <jats:italic>MYEOV and DDX60L<\/jats:italic>, which are highly expressed in PDAC cells. Notably, the knockdown of <jats:italic>MYEOV and DDX60L<\/jats:italic> significantly inhibited the metastasis of cancer cells and induced apoptosis. Further RNA sequencing analyses showed that massive signaling pathways, particularly the TNF signaling pathway and nuclear factor-kappa B (NF-\u03baB) signaling pathway, were affected in siRNA-treated cancer cells. The siDDX60L and siMYEOV significantly inhibited the expression of chemokine <jats:italic>CXCL2<\/jats:italic>, which may potentially affect the tumor microenvironment in PDAC tissues.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The present findings identified the novel oncogene <jats:italic>DDX60L<\/jats:italic>, which was highly expressed in PDAC. Transcriptome profiling through siRNA knockdown of <jats:italic>DDX60L<\/jats:italic> uncovered its functional roles in the PDAC in humans.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12864-021-08137-5","type":"journal-article","created":{"date-parts":[[2021,11,19]],"date-time":"2021-11-19T11:27:31Z","timestamp":1637321251000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Identification and functional analysis of novel oncogene DDX60L in pancreatic ductal adenocarcinoma"],"prefix":"10.1186","volume":"22","author":[{"given":"Hongjin","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Tai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuanpeng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziwei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Shui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juehua","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihua","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaosong","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,11,18]]},"reference":[{"issue":"1","key":"8137_CR1","doi-asserted-by":"publisher","first-page":"7","DOI":"10.3322\/caac.21590","volume":"70","author":"RL Siegel","year":"2020","unstructured":"Siegel RL, Miller KD, Jemal A. 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All patients provided written informed consent. This study was conducted in accordance with the tenets of the Declaration of Helsinki.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, there is no professional or other personal interest of any nature or kind in any product, service and\/or company that could be construed as influencing the position presented in the manuscript.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"833"}}