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In humans, the MBK-1 ortholog DYRK1A is associated with a variety of disorders, most prominently with neurological defects observed in Down syndrome. To better understand <jats:italic>mbk-1<\/jats:italic>\u2019s physiological roles and their dependence on genetic background, we analyzed the influence of <jats:italic>mbk-1<\/jats:italic> loss on the transcriptomes of wildtype and long-lived, germline-deficient or insulin-receptor defective, <jats:italic>C. elegans<\/jats:italic> strains by RNA-sequencing.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p><jats:italic>mbk-1<\/jats:italic> loss elicited global changes in transcription that were less pronounced in insulin-receptor mutant than in germline-deficient or wildtype <jats:italic>C. elegans<\/jats:italic>. Irrespective of genetic background, <jats:italic>mbk-1<\/jats:italic> regulated genes were enriched for functions in biological processes related to organic acid metabolism and pathogen defense. qPCR-studies confirmed <jats:italic>mbk-1<\/jats:italic> dependent induction of all three <jats:italic>C. elegans<\/jats:italic> \u03949-fatty acid desaturases, <jats:italic>fat-5<\/jats:italic>, <jats:italic>fat-6<\/jats:italic> and <jats:italic>fat-7<\/jats:italic>, in wildtype, germline-deficient and insulin-receptor mutant strains. Conversely, <jats:italic>mbk-1<\/jats:italic> dependent expression patterns of selected pathogen resistance genes, including <jats:italic>asp-12<\/jats:italic>, <jats:italic>dod-24<\/jats:italic> and <jats:italic>drd-50<\/jats:italic>, differed across the genetic backgrounds examined. Finally, <jats:italic>cth-1<\/jats:italic> and <jats:italic>cysl-2<\/jats:italic>, two genes which connect pathogen resistance to the metabolism of the gaseous messenger and lifespan regulator hydrogen sulfide (H<jats:sub>2<\/jats:sub>S), were commonly suppressed by <jats:italic>mbk-1<\/jats:italic> loss only in wildtype and germline-deficient, but not in insulin-receptor mutant <jats:italic>C. elegans<\/jats:italic>.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>Our work reveals previously unknown roles of <jats:italic>C. elegans mbk-1<\/jats:italic> in the regulation of fatty acid desaturase- and H<jats:sub>2<\/jats:sub>S metabolic-genes. These roles are only partially dependent on genetic background. Considering the particular importance of fatty acid desaturation and H<jats:sub>2<\/jats:sub>S for longevity of germline-deficient <jats:italic>C. elegans<\/jats:italic>, we propose that these processes at least in part account for the previous observation that <jats:italic>mbk-1<\/jats:italic> preferentially regulates lifespan in these worms.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12864-021-08176-y","type":"journal-article","created":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T10:03:23Z","timestamp":1641290603000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Regulation of fatty acid desaturase- and immunity gene-expression by mbk-1\/DYRK1A in Caenorhabditis elegans"],"prefix":"10.1186","volume":"23","author":[{"given":"Hildegard I. 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