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The mosquito exhibits typical sexually dimorphic behaviors such as courtship, mating, host seeking, bloodfeeding, and oviposition. All these behaviors are mainly regulated by the brain; however, little is known about the function and neuron composition of the mosquito brain. In this study, we generated an initial atlas of the adult male and female brain of <jats:italic>Ae. aegypti<\/jats:italic> using 10xGenomics based single-nucleus RNA sequencing.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>We identified 35 brain cell clusters in male and female brains, and 15 of those clusters were assigned to known cell types. Identified cell types include glia (astrocytes), Kenyon cells, (ventral) projection neurons, monoaminergic neurons, medulla neurons, and proximal medulla neurons. In addition, the cell type compositions of male and female brains were compared to each other showing that they were quantitatively distinct, as 17 out of 35 cell clusters varied significantly in their cell type proportions. Overall, the transcriptomes from each cell cluster looked very similar between the male and female brain as only up to 25 genes were differentially expressed in these clusters. The sex determination factor <jats:italic>Nix<\/jats:italic> was highly expressed in neurons and glia of the male brain, whereas <jats:italic>doublesex<\/jats:italic> (<jats:italic>dsx<\/jats:italic>) was expressed in all neuron and glia cell clusters of the male and female brain.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>An initial cell atlas of the brain of the mosquito <jats:italic>Ae. aegypti<\/jats:italic> has been generated showing that the cellular compositions of the male and female brains of this hematophagous insect differ significantly from each other. Although some of the rare brain cell types have not been detected in our single biological replicate, this study provides an important basis for the further development of a complete brain cell atlas as well as a better understanding of the neurobiology of the brains of male and female mosquitoes and their sexually dimorphic behaviors.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12864-022-08327-9","type":"journal-article","created":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T19:03:38Z","timestamp":1644519818000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Cellular diversity and gene expression profiles in the male and female brain of Aedes aegypti"],"prefix":"10.1186","volume":"23","author":[{"given":"Yingjun","family":"Cui","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanta K.","family":"Behura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander W. 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