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However, traditional grazing sheep often experience mineral nutrient deficiencies, especially copper (Cu), due to inadequate mineral nutrients from natural pastures.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The results indicated that dietary Cu deficiency and supplementation significantly reduced and elevated liver concentration of Cu, respectively (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). <jats:italic>FOXO3<\/jats:italic>, <jats:italic>PLIN1<\/jats:italic>, <jats:italic>ACTN2<\/jats:italic>, and <jats:italic>GHRHR<\/jats:italic> were identified as critical genes using the weighted gene co-expression network analysis (WGCNA), quantitative real-time polymerase chain reaction (qRT-PCR), and receiver operating characteristic curve (ROC) validation as potential biomarkers for evaluating Cu status in grazing sheep. Combining these critical genes with gene functional enrichment analysis, it was observed that dietary Cu deficiency may impair liver regeneration and compromise ribosomal function. Conversely, dietary Cu supplementation may enhance ribosomal function, promote lipid accumulation, and stimulate growth and metabolism in grazing sheep. Metabolomics analysis indicated that dietary Cu deficiency significantly decreased the abundance of metabolites such as cholic acid (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). On the other hand, dietary Cu supplementation significantly increased the abundance of metabolites such as palmitic acid (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Integrative analysis of the transcriptome and metabolome revealed that dietary Cu deficiency may reduce liver lipid metabolism while Cu supplementation may elevate it in grazing sheep.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>The Cu content in diets may have an impact on hepatic lipid metabolism in grazing sheep. These findings provide new insights into the consequences of dietary Cu deficiency and supplementation on sheep liver and can provide valuable guidance for herders to rationalize the use of mineral supplements.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12864-024-10134-3","type":"journal-article","created":{"date-parts":[[2024,2,27]],"date-time":"2024-02-27T15:02:34Z","timestamp":1709046154000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Transcriptomics and metabolomics analysis reveal the dietary copper deficiency and supplementation effects of liver gene expression and metabolite change in grazing sheep"],"prefix":"10.1186","volume":"25","author":[{"given":"Xiwei","family":"Jin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingbo","family":"Meng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Mi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,2,27]]},"reference":[{"key":"10134_CR1","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1016\/j.jes.2020.06.024","volume":"101","author":"Q Zhang","year":"2021","unstructured":"Zhang Q, Que M, Li W, Gao S, Tan X, Bu D. 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