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A variety of spatial technologies are now commercially available, each offering distinct technical parameters such as cellular resolution, detection sensitivity, gene coverage, and throughput. This wide range of options can make it challenges or create confusion for researchers to select the most appropriate platform for their specific research objectives. In this paper, we will analyze and compare seven major commercially available spatial platforms to guide researchers in choosing the most suitable option for their needs.<\/jats:p>","DOI":"10.1186\/s12864-025-11235-3","type":"journal-article","created":{"date-parts":[[2025,1,20]],"date-time":"2025-01-20T03:51:30Z","timestamp":1737345090000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":99,"title":["A practical guide for choosing an optimal spatial transcriptomics technology from seven major commercially available options"],"prefix":"10.1186","volume":"26","author":[{"given":"Hyun Ju","family":"Lim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton","family":"Buzdin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinmin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,1,20]]},"reference":[{"issue":"1","key":"11235_CR1","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1038\/nrg3832","volume":"16","author":"N Crosetto","year":"2015","unstructured":"Crosetto N, Bienko M, van Oudenaarden A. 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