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This protein is known to regulate nitrogen metabolism, contribute to nitrite susceptibility and determine virulence in aspergilli. We hypothesized that MeaB is required for proper nitric oxide (NO) metabolism of fungi. Here, we tested this hypothesis on the human pathogenic fungus <jats:italic>Aspergillus fumigatus<\/jats:italic> using a transcriptomics approach.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Deletion of the <jats:italic>meaB<\/jats:italic> gene increased nitrite, diethylamine NONOate and menadione-sodium bisulfite stress sensitivity, but not that of <jats:italic>terc<\/jats:italic>-butyl hydroperoxide or H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>. The conidia of the <jats:italic>\u0394meaB<\/jats:italic> mutant showed no altered susceptibility to human macrophages. When the transcriptome of the <jats:italic>\u0394meaB<\/jats:italic> strain was compared with the wild-type strain (Af293), genes involved in siderophore biosynthesis or glucanases were enriched in the up-regulated gene set, whereas genes encoding heme-binding proteins or chitinases were enriched in the down-regulated gene set. The 90 mM NaNO<jats:sub>2<\/jats:sub>-induced stress elicited a response in the <jats:italic>\u0394meaB<\/jats:italic> gene deletion mutant that was very similar to that of the wild-type strain in the presence of 135 mM NaNO<jats:sub>2<\/jats:sub>. These stress responses included the downregulation of mitotic cell cycle and ribosomal protein genes, and the upregulation of nitrosative stress response (<jats:italic>fhpA<\/jats:italic>,<jats:italic> fhpB<\/jats:italic>,<jats:italic> gnoA<\/jats:italic>), nitrate utilization (<jats:italic>niaD<\/jats:italic>,<jats:italic> niiA<\/jats:italic>), several iron acquisition and ergosterol biosynthesis genes, as well as the alternative oxidase gene <jats:italic>aoxA<\/jats:italic>. These stress treatments also altered the transcriptional activity of secondary metabolite cluster and carbohydrate-active enzyme (CAZyme) genes. Nitrite treatment upregulated arginine metabolism genes only in the wild-type strain. The observed transcriptional changes were associated with reduced growth, increased redox imbalance, increased sterol content in both strains and increased nitrite sensitivity of the <jats:italic>\u0394meaB<\/jats:italic> mutant on arginine as sole C and N source.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>Transcriptomic data implies that MeaB affected fine-tuned regulation of arginine metabolism genes, and the alteration in arginine dependent processes (including siderophore production and possibly NO homeostasis) was responsible for the altered phenotype of the <jats:italic>\u0394meaB<\/jats:italic> mutant. Our results also suggest that, although inhibition of <jats:italic>A. fumigatus<\/jats:italic> defense against nitrosative stress may not be an effective antifungal therapy for all <jats:italic>A. fumigatus<\/jats:italic> strains, a combined approach based on disruption of both iron and NO homeostasis is promising.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1186\/s12864-025-11990-3","type":"journal-article","created":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T13:30:38Z","timestamp":1759152638000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The MeaB bZIP transcription factor is needed for proper nitrosative stress response induced by nitrite in Aspergillus fumigatus"],"prefix":"10.1186","volume":"26","author":[{"given":"Kinga Edina","family":"Varga","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zsigmond","family":"Benk\u0151","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K\u00e1roly","family":"Antal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kitti","family":"P\u00e1zm\u00e1ndi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zolt\u00e1n","family":"Palczert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Istv\u00e1n","family":"P\u00f3csi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tam\u00e1s","family":"Emri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,9,29]]},"reference":[{"key":"11990_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/j.simyco.2021.100115","author":"A Arastehfar","year":"2021","unstructured":"Arastehfar A, Carvalho A, Houbraken J, Lombardi L, Garcia-Rubio R, Jenks JD, Rivero-Menendez O, Aljohani R, Jacobsen ID, Berman J, Osherov N, Hedayati MT, Ilkit M, Armstrong-James D, Gabald\u00f3n T, Meletiadis J, Kostrzewa M, Pan W, Lass-Fl\u00f6rl C, Perlin DS, Hoenigl M. 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