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Genomic approaches have become essential for distinguishing between local transmission and imported infections. The state of Sarawak, Malaysia, provides a pertinent example of a malaria-eliminating setting under pressure from\n                      <jats:italic>Plasmodium<\/jats:italic>\n                      parasite importation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In this study, we analysed 21\n                      <jats:italic>Plasmodium falciparum<\/jats:italic>\n                      isolates obtained from archived whole blood samples collected between 2008 and 2010 and compared them to 9,518 publicly available isolates from Central Africa (518), East Africa (849), Horn of Africa (25), Oceania (349), South America (75), South Asia (404) Southeast Asia (5,182) and West Africa (2,116). By applying nanopore sequencing and population genomic analyses, we found that most of the cases (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200913\/15) likely originated from endemic regions outside Malaysia, supported by patient travel histories and high multiplicity of infection levels. These findings and drug resistance profiles are consistent with the historical epidemiology of the suspected source regions. Notably, two cases showed genomic evidence of origins inconsistent with the patients\u2019 reported travel histories, underscoring the limitations of traditional epidemiological methods. Identity-by-descent analysis revealed clustering in only two cases, indicating that the majority of infections were likely isolated introductions rather than evidence of sustained local transmission.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Overall, our results highlight the power of malaria genomics in discerning imported from locally acquired cases and emphasise its critical role in maintaining malaria elimination, particularly in regions situated along major migration and labour exchange corridors.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12864-026-12653-7","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T02:14:09Z","timestamp":1771553649000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Whole genome sequencing to inform the epidemiology of Plasmodium falciparum malaria in the elimination setting of Malaysia"],"prefix":"10.1186","volume":"27","author":[{"given":"Mark K. 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