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All sample collection procedures were conducted using non-lethal sampling methods, in accordance with institutional laboratory regulations and established conservation guidelines for critically endangered anuran species. Field sampling activities were conducted under permission of the Ministry of Agriculture, Forestry, and Fisheries of Grenada, with oversight by the Office of the Chief Veterinary Officer, in compliance with national wildlife regulations. No animal was euthanized, sacrificed, or anesthetized at any stage of this study. A single wild female was sampled non-lethally by toe clip, buccal swab, and blood swab, following the IUCN Policy Statement on Research Involving Species at Risk of Extinction [\n                      \n                      ]. The two toe-clip sites were disinfected with diluted povidone-iodine before and after clipping, and the animal was confirmed to have achieved hemostasis and to be responsive before being released at the exact location of capture. 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