{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T07:12:53Z","timestamp":1781853173220,"version":"3.54.5"},"reference-count":33,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,6,27]],"date-time":"2021-06-27T00:00:00Z","timestamp":1624752000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,6,27]],"date-time":"2021-06-27T00:00:00Z","timestamp":1624752000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Immunol"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p><jats:italic>Pneumocystis<\/jats:italic> pneumonia (PcP), which is caused by <jats:italic>Pneumocystis carinii,<\/jats:italic> is a life-threatening infection that affects immunocompromised individuals. Unfortunately, chemoprophylaxis and dapsone are only effective for half of the patients with PcP, indicating that additional preventive methods are needed. We predicated the <jats:italic>pneumocystis<\/jats:italic> surface protein A12 sequence 1\u201385 by DNAStar software and BepiPred, and identified it as a potential vaccine candidate by bioresearch.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>We used recombinant A12<jats:sub>1\u201385<\/jats:sub> as antigen to immunized mice and detected serum titer of IgG, expression of inflammatory factors by EILSA, qRT-PCR and flow cytometry.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Our results showed that immunization with recombinant A12<jats:sub>1\u201385<\/jats:sub> increased the serum titer of IgG, promoted the secretion of T lymphocytes, increased the expression of inflammatory factors, and elevated lung inflammatory injury in mice.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Our findings suggest that A12<jats:sub>1\u201385<\/jats:sub> is a potential vaccine target for preventing <jats:italic>Pneumocystis carinii<\/jats:italic>. The evaluation of A12<jats:sub>1\u201385<\/jats:sub>-elicited antibodies in the prevention of PcP in humans deserves further investigation.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12865-021-00436-6","type":"journal-article","created":{"date-parts":[[2021,6,26]],"date-time":"2021-06-26T23:04:23Z","timestamp":1624748663000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Immunization with Pneumocystis carinii A121\u201385 antigen activates immune function against P. carinii"],"prefix":"10.1186","volume":"22","author":[{"given":"Tong","family":"Tong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongxin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanhong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jilu","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,6,27]]},"reference":[{"key":"436_CR1","doi-asserted-by":"publisher","unstructured":"Corallo CE, Coutsouvelis J, Morgan S, Morrissey O, Avery S. 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The study was carried out in compliance with the ARRIVE guidelines. All protocols were approved by the Animal Ethics Committee of Anhui Medical University (No. 20200938).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"40"}}