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Peptides to represent these B-cell epitopes were obtained from commercially available arrays, and were subjected to enzyme linked immunosorbent assay with sera obtained from dengue seropositive healthy volunteers (DENV1 n\u2009=\u200912: DENV2 n\u2009=\u200912: DENV3 n\u2009=\u200912 and DENV4 n\u2009=\u200912), and 10 dengue seronegative healthy volunteers from Sri Lanka. The cut-off value for the positive antibody response was set by taking the mean response of a peptide to the negative sera plus three standard deviations. The peptides (N\u00a0=\u00a07)\u00a0showing the broad immune responses were used to generate antibodies in three mice (Balb\/c) batches. The mice antisera were then subjected to microneutralization assays against all the four DENV serotypes. An EC<jats:sub>50<\/jats:sub>viral neutralization\u2009\u2265\u200940 times the serum dilution was considered as neutralizing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Five of the E-peptide and two prM peptides were recognised by most individuls exposed to infections with each of the four serotypes, showing a serotype cross-reactive broad antibody response.\u00a0The mice immune sera against the peptides representing the five E protein epitopes neutralized all the four DENV serotypes. Two of these five epitopes are from the Domain II, whereas one of them includes the whole bc-loop region.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The antibody responses of highly conserved epitopes across the serotypes, were broadly responsive with sera of all four DENV serotypes collected from individuals infected with only one DENV serotype. Weakly conserved epitopes showed rather specific antibody responses dominated by one or few serotypes.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12865-021-00462-4","type":"journal-article","created":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T13:03:47Z","timestamp":1635944627000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Natural immunogenic properties of bioinformatically predicted linear B-cell epitopes of dengue envelope and pre-membrane proteins"],"prefix":"10.1186","volume":"22","author":[{"given":"Mahesha N.","family":"Nadugala","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandima","family":"Jeewandara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramesh S.","family":"Jadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gathsaurie N.","family":"Malavige","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aravinda M.","family":"de Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prasad H.","family":"Premaratne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charitha L.","family":"Goonasekara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,11,3]]},"reference":[{"key":"462_CR1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12060","author":"S Bhatt","year":"2013","unstructured":"Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, et al. 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In addition, informed written consent was obtained from all the adult subjects, and the parents\/guardians of the child participants, prior to the collection of blood. The Ethical Review Committee of Faculty of Medicine, General Sir John Kotelawala Defence University reviewed and approved the protocols on animal studies (RP\/2014\/01), which adhered to the guidelines by International Council of Laboratory Animal Science (ICLAS).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"Intellectual properties protected by two national patents; Patent No: 19832\u2014An epitope-based vaccine target with viral neutralization potential for dengue disease. Patent No: 19833\u2014A diagnostic marker for specific identification of the dengue viruses.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Disclosure"}}],"article-number":"71"}}