{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:34:05Z","timestamp":1785890045348,"version":"3.56.0"},"reference-count":38,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2015,5,13]],"date-time":"2015-05-13T00:00:00Z","timestamp":1431475200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2015,12]]},"DOI":"10.1186\/s12866-015-0431-7","type":"journal-article","created":{"date-parts":[[2015,5,13]],"date-time":"2015-05-13T15:55:45Z","timestamp":1431532545000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Heat fixation inactivates viral and bacterial pathogens and is compatible with downstream MALDI mass spectrometry tissue imaging"],"prefix":"10.1186","volume":"15","author":[{"given":"Lisa H","family":"Cazares","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean A","family":"Van Tongeren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Costantino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tara","family":"Kenny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole L","family":"Garza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ginger","family":"Donnelly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas","family":"Lane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rekha G","family":"Panchal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sina","family":"Bavari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,5,13]]},"reference":[{"issue":"6","key":"431_CR1","doi-asserted-by":"publisher","first-page":"1489","DOI":"10.1074\/mcp.R112.024869","volume":"12","author":"K Sk\u00f6ld","year":"2013","unstructured":"Sk\u00f6ld K, Alm H, Scholz B. The impact of biosampling procedures on molecular data interpretation. Molecular & cellular proteomics : MCP. 2013;12(6):1489\u2013501.","journal-title":"Molecular & cellular proteomics : MCP"},{"issue":"1","key":"431_CR2","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1007\/s00280-007-0440-9","volume":"61","author":"RJ Jones","year":"2008","unstructured":"Jones RJ, Boyce T, Fennell M, Jacobs V, Pinto F, Duffield E, et al. The impact of delay in cryo-fixation on biomarkers of Src tyrosine kinase activity in human breast and bladder cancers. Cancer Chemother Pharmacol. 2008;61(1):23\u201332.","journal-title":"Cancer Chemother Pharmacol"},{"issue":"6","key":"431_CR3","doi-asserted-by":"publisher","first-page":"2807","DOI":"10.1021\/pr401232e","volume":"13","author":"C Stingl","year":"2014","unstructured":"Stingl C, S\u00f6derquist M, Karlsson O, Bor\u00e9n M, Luider TM. Uncovering effects of ex-vivo protease activity during proteomics and peptidomics sample extraction in rat brain tissue by oxygen-18 labelling. J Proteome Res. 2014;13(6):2807\u201317.","journal-title":"J Proteome Res"},{"issue":"18","key":"431_CR4","doi-asserted-by":"publisher","first-page":"3702","DOI":"10.1002\/pmic.200701143","volume":"8","author":"M Ronci","year":"2008","unstructured":"Ronci M, Bonanno E, Colantoni A, Pieroni L, Di Ilio C, Spagnoli LG, et al. Protein unlocking procedures of formalin-fixed paraffin-embedded tissues: application to MALDI-TOF imaging MS investigations. Proteomics. 2008;8(18):3702\u201314.","journal-title":"Proteomics"},{"issue":"14","key":"431_CR5","doi-asserted-by":"publisher","first-page":"2941","DOI":"10.1039\/c0an00269k","volume":"136","author":"MJ Hackett","year":"2011","unstructured":"Hackett MJ, McQuillan JA, El-Assaad F, Aitken JB, Levina A, Cohen DD, et al. Chemical alterations to murine brain tissue induced by formalin fixation: implications for biospectroscopic imaging and mapping studies of disease pathogenesis. Analyst. 2011;136(14):2941\u201352.","journal-title":"Analyst"},{"issue":"8","key":"431_CR6","doi-asserted-by":"publisher","first-page":"3543","DOI":"10.1021\/pr800286z","volume":"7","author":"P Chaurand","year":"2008","unstructured":"Chaurand P, Latham JC, Lane KB, Mobley JA, Polosukhin VV, Wirth PS, et al. Imaging mass spectrometry of intact proteins from alcohol-preserved tissue specimens: bypassing formalin fixation. J Proteome Res. 2008;7(8):3543\u201355.","journal-title":"J Proteome Res"},{"issue":"18","key":"431_CR7","doi-asserted-by":"publisher","first-page":"3801","DOI":"10.1002\/pmic.200800201","volume":"8","author":"RJ Goodwin","year":"2008","unstructured":"Goodwin RJ, Dungworth JC, Cobb SR, Pitt AR. Time-dependent evolution of tissue markers by MALDI-MS imaging. Proteomics. 2008;8(18):3801\u20138.","journal-title":"Proteomics"},{"issue":"9","key":"431_CR8","doi-asserted-by":"publisher","first-page":"1751","DOI":"10.1002\/pmic.200900641","volume":"10","author":"RJ Goodwin","year":"2010","unstructured":"Goodwin RJ, Lang AM, Allingham H, Bor\u00e9n M, Pitt AR. Stopping the clock on proteomic degradation by heat treatment at the point of tissue excision. Proteomics. 2010;10(9):1751\u201361.","journal-title":"Proteomics"},{"issue":"3","key":"431_CR9","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1136\/jcp.23.3.273","volume":"23","author":"CP Mayers","year":"1970","unstructured":"Mayers CP. Histological fixation by microwave heating. J Clin Pathol. 1970;23(3):273\u20135.","journal-title":"J Clin Pathol"},{"issue":"2","key":"431_CR10","doi-asserted-by":"publisher","first-page":"974","DOI":"10.1021\/pr8006446","volume":"8","author":"M Svensson","year":"2009","unstructured":"Svensson M, Bor\u00e9n M, Sk\u00f6ld K, F\u00e4lth M, Sj\u00f6gren B, Andersson M, et al. Heat stabilization of the tissue proteome: a new technology for improved proteomics. J Proteome Res. 2009;8(2):974\u201381.","journal-title":"J Proteome Res"},{"issue":"1","key":"431_CR11","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/j.jneumeth.2011.01.004","volume":"196","author":"MM Ahmed","year":"2011","unstructured":"Ahmed MM, Gardiner KJ. Preserving protein profiles in tissue samples: differing outcomes with and without heat stabilization. J Neurosci Methods. 2011;196(1):99\u2013106.","journal-title":"J Neurosci Methods"},{"issue":"1","key":"431_CR12","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/j.jprot.2011.06.009","volume":"75","author":"K Kultima","year":"2011","unstructured":"Kultima K, Sk\u00f6ld K, Bor\u00e9n M. Biomarkers of disease and post-mortem changes - Heat stabilization, a necessary tool for measurement of protein regulation. J Proteomics. 2011;75(1):145\u201359.","journal-title":"J Proteomics"},{"issue":"2","key":"431_CR13","doi-asserted-by":"publisher","first-page":"743","DOI":"10.1021\/pr300805f","volume":"12","author":"RM Sturm","year":"2013","unstructured":"Sturm RM, Greer T, Woodards N, Gemperline E, Li L. Mass spectrometric evaluation of neuropeptidomic profiles upon heat stabilization treatment of neuroendocrine tissues in crustaceans. J Proteome Res. 2013;12(2):743\u201352.","journal-title":"J Proteome Res"},{"issue":"3","key":"431_CR14","doi-asserted-by":"publisher","first-page":"303","DOI":"10.1002\/jms.1537","volume":"44","author":"ML Vestal","year":"2009","unstructured":"Vestal ML. Modern MALDI time-of-flight mass spectrometry. Journal of mass spectrometry : JMS. 2009;44(3):303\u201317.","journal-title":"Journal of mass spectrometry : JMS"},{"issue":"4","key":"431_CR15","doi-asserted-by":"publisher","first-page":"476","DOI":"10.1002\/jms.1180","volume":"42","author":"P Chaurand","year":"2007","unstructured":"Chaurand P, Schriver KE, Caprioli RM. Instrument design and characterization for high resolution MALDI-MS imaging of tissue sections. Journal of mass spectrometry : JMS. 2007;42(4):476\u201389.","journal-title":"Journal of mass spectrometry : JMS"},{"issue":"1","key":"431_CR16","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1016\/j.mad.2004.09.032","volume":"126","author":"TC Rohner","year":"2005","unstructured":"Rohner TC, Staab D, Stoeckli M. MALDI mass spectrometric imaging of biological tissue sections. Mech Ageing Dev. 2005;126(1):177\u201385.","journal-title":"Mech Ageing Dev"},{"issue":"6","key":"431_CR17","doi-asserted-by":"publisher","first-page":"884","DOI":"10.1046\/j.1365-2672.1999.00935.x","volume":"87","author":"L Croci","year":"1999","unstructured":"Croci L, Ciccozzi M, De Medici D, Di Pasquale S, Fiore A, Mele A, et al. Inactivation of hepatitis A virus in heat-treated mussels. J Appl Microbiol. 1999;87(6):884\u20138.","journal-title":"J Appl Microbiol"},{"issue":"3","key":"431_CR18","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1089\/fpd.2010.0681","volume":"8","author":"H Sow","year":"2011","unstructured":"Sow H, Desbiens M, Morales-Rayas R, Ngazoa SE, Jean J. Heat inactivation of hepatitis A virus and a norovirus surrogate in soft-shell clams (Mya arenaria). Foodborne Pathog Dis. 2011;8(3):387\u201393.","journal-title":"Foodborne Pathog Dis"},{"key":"431_CR19","doi-asserted-by":"crossref","unstructured":"Aguirre JS, Pin C, Rodriguez MR, Garcia de Fernando GD. Analysis of the variability in the number of viable bacteria after mild heat treatment of food. Appl Environ Microbiol. 2009;75(22):6992\u20137.","DOI":"10.1128\/AEM.00452-09"},{"issue":"3","key":"431_CR20","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1128\/JVI.67.3.1269-1277.1993","volume":"67","author":"RM Kinney","year":"1993","unstructured":"Kinney RM, Chang GJ, Tsuchiya KR, Sneider JM, Roehrig JT, Woodward TM, et al. Attenuation of Venezuelan equine encephalitis virus strain TC-83 is encoded by the 5\u2019-noncoding region and the E2 envelope glycoprotein. J Virol. 1993;67(3):1269\u201377.","journal-title":"J Virol"},{"issue":"Pt 12","key":"431_CR21","doi-asserted-by":"publisher","first-page":"1109","DOI":"10.1099\/jmm.0.05180-0","volume":"52","author":"MS Lever","year":"2003","unstructured":"Lever MS, Nelson M, Ireland PI, Stagg AJ, Beedham RJ, Hall GA, et al. Experimental aerogenic Burkholderia mallei (glanders) infection in the BALB\/c mouse. J Med Microbiol. 2003;52(Pt 12):1109\u201315.","journal-title":"J Med Microbiol"},{"issue":"1","key":"431_CR22","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/j.jasms.2006.08.015","volume":"18","author":"SN Jackson","year":"2007","unstructured":"Jackson SN, Wang HY, Woods AS. In situ structural characterization of glycerophospholipids and sulfatides in brain tissue using MALDI-MS\/MS. J Am Soc Mass Spectrom. 2007;18(1):17\u201326.","journal-title":"J Am Soc Mass Spectrom"},{"issue":"5","key":"431_CR23","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1016\/j.chemphyslip.2012.06.001","volume":"165","author":"LJ Sparvero","year":"2012","unstructured":"Sparvero LJ, Amoscato AA, Dixon CE, Long JB, Kochanek PM, Pitt BR, et al. Mapping of phospholipids by MALDI imaging (MALDI-MSI): realities and expectations. Chem Phys Lipids. 2012;165(5):545\u201362.","journal-title":"Chem Phys Lipids"},{"issue":"14","key":"431_CR24","doi-asserted-by":"publisher","first-page":"5001","DOI":"10.1039\/c4ay00320a","volume":"6","author":"SN Jackson","year":"2014","unstructured":"Jackson SN, Barbacci D, Egan T, Lewis EK, Schultz JA, Woods AS. MALDI-Ion Mobility Mass Spectrometry of Lipids in Negative Ion Mode. Analytical methods : advancing methods and applications. 2014;6(14):5001\u20137.","journal-title":"Analytical methods : advancing methods and applications"},{"issue":"3","key":"431_CR25","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M900229-MCP200","volume":"10","author":"B Scholz","year":"2011","unstructured":"Scholz B, Sk\u00f6ld K, Kultima K, Fernandez C, Waldemarson S, Savitski MM, et al. Impact of temperature dependent sampling procedures in proteomics and peptidomics\u2013a characterization of the liver and pancreas post mortem degradome. Molecular & cellular proteomics: MCP. 2011;10(3):M900229-MCP900200-900221.","journal-title":"Molecular & cellular proteomics: MCP"},{"key":"431_CR26","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1186\/1477-5956-8-61","volume":"8","author":"CB Rountree","year":"2010","unstructured":"Rountree CB, Van Kirk CA, You H, Ding W, Dang H, VanGuilder HD, et al. Clinical application for the preservation of phospho-proteins through in-situ tissue stabilization. Proteome Sci. 2010;8:61.","journal-title":"Proteome Sci"},{"issue":"16","key":"431_CR27","doi-asserted-by":"publisher","first-page":"4912","DOI":"10.1016\/j.jprot.2012.07.006","volume":"75","author":"RJ Goodwin","year":"2012","unstructured":"Goodwin RJ, Nilsson A, Borg D, Langridge-Smith PR, Harrison DJ, Mackay CL, et al. Conductive carbon tape used for support and mounting of both whole animal and fragile heat-treated tissue sections for MALDI MS imaging and quantitation. J Proteomics. 2012;75(16):4912\u201320.","journal-title":"J Proteomics"},{"issue":"18","key":"431_CR28","doi-asserted-by":"publisher","first-page":"3785","DOI":"10.1002\/pmic.200800320","volume":"8","author":"RJ Goodwin","year":"2008","unstructured":"Goodwin RJ, Pennington SR, Pitt AR. Protein and peptides in pictures: imaging with MALDI mass spectrometry. Proteomics. 2008;8(18):3785\u2013800.","journal-title":"Proteomics"},{"issue":"3","key":"431_CR29","doi-asserted-by":"crossref","first-page":"285","DOI":"10.4315\/0362-028X-61.3.285","volume":"61","author":"MR Clavero","year":"1998","unstructured":"Clavero MR, Beuchat LR, Doyle MP. Thermal inactivation of Escherichia coli O157:H7 isolated from ground beef and bovine feces, and suitability of media for enumeration. J Food Prot. 1998;61(3):285\u20139.","journal-title":"J Food Prot"},{"issue":"3","key":"431_CR30","doi-asserted-by":"crossref","first-page":"674","DOI":"10.4315\/0362-028X-70.3.674","volume":"70","author":"C Thomas","year":"2007","unstructured":"Thomas C, Swayne DE. Thermal inactivation of H5N1 high pathogenicity avian influenza virus in naturally infected chicken meat. J Food Prot. 2007;70(3):674\u201380.","journal-title":"J Food Prot"},{"issue":"5","key":"431_CR31","doi-asserted-by":"publisher","first-page":"1050","DOI":"10.1111\/j.1365-2672.2012.05282.x","volume":"112","author":"E Tuladhar","year":"2012","unstructured":"Tuladhar E, Bouwknegt M, Zwietering MH, Koopmans M, Duizer E. Thermal stability of structurally different viruses with proven or potential relevance to food safety. J Appl Microbiol. 2012;112(5):1050\u20137.","journal-title":"J Appl Microbiol"},{"issue":"2","key":"431_CR32","doi-asserted-by":"publisher","first-page":"543","DOI":"10.1128\/AEM.01039-07","volume":"74","author":"L Baert","year":"2008","unstructured":"Baert L, Wobus CE, Van Coillie E, Thackray LB, Debevere J, Uyttendaele M. Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure. Appl Environ Microbiol. 2008;74(2):543\u20136.","journal-title":"Appl Environ Microbiol"},{"issue":"8","key":"431_CR33","doi-asserted-by":"publisher","first-page":"1394","DOI":"10.1007\/s13361-014-0883-2","volume":"25","author":"DM Anderson","year":"2014","unstructured":"Anderson DM, Ablonczy Z, Koutalos Y, Spraggins J, Crouch RK, Caprioli RM, et al. High resolution MALDI imaging mass spectrometry of retinal tissue lipids. J Am Soc Mass Spectrom. 2014;25(8):1394\u2013403.","journal-title":"J Am Soc Mass Spectrom"},{"issue":"7\u20138","key":"431_CR34","doi-asserted-by":"publisher","first-page":"924","DOI":"10.1002\/pmic.201300434","volume":"14","author":"EE Jones","year":"2014","unstructured":"Jones EE, Powers TW, Neely BA, Cazares LH, Troyer DA, Parker AS, et al. MALDI imaging mass spectrometry profiling of proteins and lipids in clear cell renal cell carcinoma. Proteomics. 2014;14(7\u20138):924\u201335.","journal-title":"Proteomics"},{"issue":"5","key":"431_CR35","doi-asserted-by":"publisher","first-page":"1289","DOI":"10.1039\/c2an36337b","volume":"138","author":"D Gode","year":"2013","unstructured":"Gode D, Volmer DA. Lipid imaging by mass spectrometry - a review. Analyst. 2013;138(5):1289\u2013315.","journal-title":"Analyst"},{"issue":"18","key":"431_CR36","doi-asserted-by":"publisher","first-page":"4357","DOI":"10.1007\/s00216-014-7846-0","volume":"406","author":"S Guo","year":"2014","unstructured":"Guo S, Qiu L, Wang Y, Qin X, Liu H, He M, et al. Tissue imaging and serum lipidomic profiling for screening potential biomarkers of thyroid tumors by matrix-assisted laser desorption\/ionization-Fourier transform ion cyclotron resonance mass spectrometry. Anal Bioanal Chem. 2014;406(18):4357\u201370.","journal-title":"Anal Bioanal Chem"},{"key":"431_CR37","first-page":"13","volume":"71C","author":"F Jerneren","year":"2014","unstructured":"Jerneren F, S\u00f6derquist M, Karlsson O. Post-sampling release of free fatty acids - effects of heat stabilization and methods of euthanasia. J Pharmacol Toxicol Methods. 2014;71C:13\u201320.","journal-title":"J Pharmacol Toxicol Methods"},{"issue":"7","key":"431_CR38","doi-asserted-by":"publisher","first-page":"622","DOI":"10.1002\/jms.3382","volume":"49","author":"NH Patterson","year":"2014","unstructured":"Patterson NH, Thomas A, Chaurand P. Monitoring time-dependent degradation of phospholipids in sectioned tissues by MALDI imaging mass spectrometry. Journal of mass spectrometry : JMS. 2014;49(7):622\u20137.","journal-title":"Journal of mass spectrometry : JMS"}],"container-title":["BMC Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s12866-015-0431-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12866-015-0431-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12866-015-0431-7","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12866-015-0431-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T04:17:59Z","timestamp":1599020279000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcmicrobiol.biomedcentral.com\/articles\/10.1186\/s12866-015-0431-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,13]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["431"],"URL":"https:\/\/doi.org\/10.1186\/s12866-015-0431-7","relation":{},"ISSN":["1471-2180"],"issn-type":[{"value":"1471-2180","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,5,13]]},"assertion":[{"value":"11 August 2014","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 April 2015","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 May 2015","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}],"article-number":"101"}}