{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T12:47:59Z","timestamp":1782218879543,"version":"3.54.5"},"reference-count":49,"publisher":"Springer Science and Business Media LLC","issue":"1","funder":[{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["AGL2012-34985"],"award-info":[{"award-number":["AGL2012-34985"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2015,12]]},"DOI":"10.1186\/s12866-015-0591-5","type":"journal-article","created":{"date-parts":[[2015,10,30]],"date-time":"2015-10-30T12:46:58Z","timestamp":1446209218000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["The secreted autotransporter toxin (Sat) does not act as a virulence factor in the probiotic Escherichia coli strain Nissle 1917"],"prefix":"10.1186","volume":"15","author":[{"given":"Lorena","family":"Toloza","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Gim\u00e9nez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Jose","family":"F\u00e1brega","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carina Shianya","family":"Alvarez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Aguilera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Alexandra","family":"Ca\u00f1as","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel","family":"Mart\u00edn-Venegas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josefa","family":"Badia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Baldom\u00e0","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,10,30]]},"reference":[{"issue":"11","key":"591_CR1","doi-asserted-by":"crossref","first-page":"1617","DOI":"10.1136\/gut.2003.037747","volume":"53","author":"W Kruis","year":"2004","unstructured":"Kruis W, Fric P, Pokrotnieks J, Lukas M, Fixa B, Kascak M, et al. Maintaining remission of ulcerative colitis with the probiotic Escherichia coli Nissle 1917 is as effective as with standard mesalazine. Gut. 2004;53(11):1617. 23.","journal-title":"Gut"},{"key":"591_CR2","doi-asserted-by":"crossref","first-page":"122","DOI":"10.3109\/08910600903444267","volume":"21","author":"U Sonnenborn","year":"2009","unstructured":"Sonnenborn U, Schulze J. The non-pathogenic Escherichia coli strain Nissle 1917-features of a versatile probiotic. Microb Ecol Health Dis. 2009;21:122\u201358.","journal-title":"Microb Ecol Health Dis"},{"key":"591_CR3","doi-asserted-by":"crossref","first-page":"600","DOI":"10.1159\/000333307","volume":"29","author":"CA Jacobi","year":"2011","unstructured":"Jacobi CA, Malfertheiner P. Escherichia coli Nissle 1917 (Mutaflor): new insights into an old probiotic bacterium. Dig Dis. 2011;29:600\u20137.","journal-title":"Dig Dis"},{"issue":"8","key":"591_CR4","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1111\/j.1753-4887.2010.00305.x","volume":"68","author":"I Trebichavsky","year":"2010","unstructured":"Trebichavsky I, Splichal I, Rada V, Splichalova A. Modulation of natural immunity in the gut by Escherichia coli Nissle 1917. Nutr Rev. 2010;68(8):459\u201364.","journal-title":"Nutr Rev"},{"issue":"12","key":"591_CR5","doi-asserted-by":"crossref","first-page":"e1308","DOI":"10.1371\/journal.pone.0001308","volume":"2","author":"SN Ukena","year":"2007","unstructured":"Ukena SN, Singh A, Dringenberg U, Engelhardt R, Seidler U, Hansen W. Probiotic Escherichia coli Nissle 1917 inhibits leaky gut by enhancing mucosal integrity. PLoS One. 2007;2(12):e1308.","journal-title":"PLoS One"},{"key":"591_CR6","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1111\/j.1462-5822.2006.00836.x","volume":"9","author":"A Zyrek","year":"2007","unstructured":"Zyrek A, Cichon C, Helms S, Enders C, Sonnenborn U, Schmidt MA. Molecular mechanisms underlying the probiotic effects of Escherichia coli Nissle 1917 involve ZO-2 and PKCzeta redistribution resulting in tight junction and epithelial barrier repair. Cell Microbiol. 2007;9:804\u201315.","journal-title":"Cell Microbiol"},{"key":"591_CR7","doi-asserted-by":"crossref","unstructured":"Hering NA, Richter JF, Fromm A, Wieser A, Hartmann S, G\u00fcnzel D, et al. TcpC protein from E. coli Nissle improves epithelial barrier function involving PKC\u01ba and ERK1\/2 signaling in HT-29\/B6 cells. Mucosal Immunol. 2013; doi:10.1038\/mi.2013.55","DOI":"10.1038\/mi.2013.55"},{"key":"591_CR8","doi-asserted-by":"crossref","first-page":"e0004713","DOI":"10.1128\/genomeA.00047-13","volume":"1","author":"BF Cress","year":"2013","unstructured":"Cress BF, Linhardt RJ, Koffas MA. Draft genome sequence Escherichia coli strain Nissle 1917 (Serovar O6:K5:H1). Genome Announc. 2013;1:e0004713.","journal-title":"Genome Announc"},{"key":"591_CR9","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.jbiotec.2014.07.442","volume":"187","author":"M Reister","year":"2014","unstructured":"Reister M, Hoffmeier K, Krezdorn N, Rotter B, Liang C, Rund S, et al. Complete genome sequence of the Gram-negative probiotic Escherichia coli Nissle 1917. J Biotech. 2014;187:106\u20137.","journal-title":"J Biotech"},{"key":"591_CR10","doi-asserted-by":"crossref","first-page":"5432","DOI":"10.1128\/JB.186.16.5432-5441.2004","volume":"186","author":"L Grozdanov","year":"2004","unstructured":"Grozdanov L, Raasch C, Schulze J, Sonnenborn U, Gottschalk G, Hacker J, et al. Analysis of the genome structure of the nonpathogenic probiotic Escherichia coli strain Nissle 1917. J Bacteriol. 2004;186:5432\u201341.","journal-title":"J Bacteriol"},{"key":"591_CR11","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.jbiotec.2005.01.008","volume":"117","author":"J Sun","year":"2005","unstructured":"Sun J, Gunzer F, Westendorf AM, Buer J, Scharfe M, et al. Genomic peculiarity of coding sequences and metabolic potential of probiotic Escherichia coli strain Nissle 1917 inferred from raw genome data. J Biotechnol. 2005;117:147\u201361.","journal-title":"J Biotechnol"},{"key":"591_CR12","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1007\/s00438-010-0532-9","volume":"283","author":"RB Vejborg","year":"2010","unstructured":"Vejborg RB, Friis C, Hancock V, Schembri MA, Klemm P. A virulent parent with probiotic progeny: comparative genomics of Escherichia coli strains CFT073, Nissle 1917 and ABU 83972. Mol Genet Genomics. 2010;283:469\u201384.","journal-title":"Mol Genet Genomics"},{"key":"591_CR13","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1007\/s00438-010-0578-8","volume":"284","author":"V Hancock","year":"2010","unstructured":"Hancock V, Vejborg RM, Klemm P. Functional genomics of probiotic Escherichia coli Nissle 1917 and 83972, and UPEC strain CFT073: comparison of transcriptomes, growth and biofilm formation. Mol Genet Genomics. 2010;284:437\u201354.","journal-title":"Mol Genet Genomics"},{"key":"591_CR14","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1128\/IAI.69.3.1231-1243.2001","volume":"69","author":"IR Henderson","year":"2001","unstructured":"Henderson IR, Nataro JP. Virulence funcions of autotransporter proteins. Infect Immun. 2001;69:1231\u201343.","journal-title":"Infect Immun"},{"key":"591_CR15","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1128\/MMBR.68.4.692-744.2004","volume":"68","author":"IR Henderson","year":"2004","unstructured":"Henderson IR, Navarro-Garc\u00eda F, Desvaux M, Fernandez RC, Ala\u2019Aldeen D. Type V protein secretion pathway: the autotransporter story. Microbiol Mol Biol Rev. 2004;68:692\u2013744.","journal-title":"Microbiol Mol Biol Rev"},{"key":"591_CR16","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.3390\/toxins2061179","volume":"2","author":"N Dautin","year":"2010","unstructured":"Dautin N. Serine protease autotransporters of Enterobacteriaceae (SPATEs): biogenesis and function. Toxins. 2010;2:1179\u2013206.","journal-title":"Toxins"},{"key":"591_CR17","doi-asserted-by":"crossref","unstructured":"Ruiz-Perez F, Nataro JP. Bacterial serine proteases secreted by the autotransporter pathway: classification, specificity and role in virulence. Cell Mol Life Sci. 2013; doi:10.1007\/s00018-013-1355-8","DOI":"10.1007\/s00018-013-1355-8"},{"key":"591_CR18","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1046\/j.1365-2958.2000.02110.x","volume":"38","author":"DM Guyer","year":"2000","unstructured":"Guyer DM, Henderson IR, Nataro JP, Mobley HL. Identification of sat, an autotransporter toxin produced by uropathogenic Escherichia coli. Mol Microbiol. 2000;38:53\u201366.","journal-title":"Mol Microbiol"},{"key":"591_CR19","doi-asserted-by":"crossref","first-page":"7105","DOI":"10.1128\/IAI.70.12.7105-7113.2002","volume":"70","author":"PR Dutta","year":"2002","unstructured":"Dutta PR, Capello R, Navarro-Garc\u00eda F, Nataro JP. Functional comparison of serine protease autotransporters of enterobacteriaceae. Infect Immun. 2002;70:7105\u201313.","journal-title":"Infect Immun"},{"issue":"11","key":"591_CR20","doi-asserted-by":"crossref","first-page":"6124","DOI":"10.1128\/IAI.01086-06","volume":"74","author":"NM Maroncle","year":"2006","unstructured":"Maroncle NM, Sivick KE, Brady R, Stokes F-E, Mobley HLT. Protease activity, secretion, cell entry, cytotoxicity, and cellular targets of secreted autotransporter toxin of uropathogenic. Infect Immun. 2006;74(11):6124\u201334.","journal-title":"Infect Immun"},{"issue":"8","key":"591_CR21","doi-asserted-by":"crossref","first-page":"4539","DOI":"10.1128\/IAI.70.8.4539-4546.2002","volume":"70","author":"DM Guyer","year":"2002","unstructured":"Guyer DM, Radulovic S, Jones FE, Mobley HL. Sat, the secreted autotransporter toxin of uropathogenic Escherichia coli, is a vacuolating cytotoxin for bladder and kidney epithelial cells. Infect Immun. 2002;70(8):4539\u201346.","journal-title":"Infect Immun"},{"issue":"7","key":"591_CR22","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1111\/j.1462-5822.2011.01595.x","volume":"13","author":"V Li\u00e9vin-Le Mola","year":"2011","unstructured":"Li\u00e9vin-Le Mola V, Comenge Y, Ruby V, Amsellem R, Nicolas V, Servin AL. Secreted autotransporter toxin (Sat) triggers autophagy in epithelial cells that relies on cell detachment. Cell Microbiol. 2011;13(7):992\u20131013.","journal-title":"Cell Microbiol"},{"issue":"1","key":"591_CR23","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1111\/j.1462-5822.2006.00782.x","volume":"9","author":"J Guignot","year":"2007","unstructured":"Guignot J, Chaplais C, Coconnier-Polter MH, Servin AL. The secreted autotransporter toxin, Sat, functions as a virulence factor in Afa\/Dr diffusely adhering Escherichia coli by promoting lesions in tight junction of polarized epithelial cells. Cell Microbiol. 2007;9(1):204\u201321.","journal-title":"Cell Microbiol"},{"issue":"2","key":"591_CR24","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.femsle.2005.07.013","volume":"250","author":"CR Taddei","year":"2005","unstructured":"Taddei CR, Fasano A, Ferreira AJ, Trabulsi LR, Martinez MB. Secreted autotransporter toxin produced by a diffusely adhering Escherichia coli strain causes intestinal damage in animal model assays. FEMS Microbiol Lett. 2005;250(2):263\u20139.","journal-title":"FEMS Microbiol Lett"},{"key":"591_CR25","doi-asserted-by":"crossref","first-page":"5912","DOI":"10.1128\/JB.184.21.5912-5925.2002","volume":"184","author":"L Grozdanov","year":"2002","unstructured":"Grozdanov L, Z\u00e4hringer U, Blum-Oehler G, Brade L, Henne A, Knirel YA, et al. A single nucleotide exchange in the wzy gene is responsible for the semirough O6 lipopolysaccharide phenotype and serum sensitivity of Escherichia coli strain Nissle 1917. J Bacteriol. 2002;184:5912\u201325.","journal-title":"J Bacteriol"},{"key":"591_CR26","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1128\/JB.140.2.320-326.1979","volume":"140","author":"A Boronat","year":"1979","unstructured":"Boronat A, Aguilar J. Rhamnose-induced propanediol oxidoreductase in Escherichia coli: purification, properties, and comparison with the fucose-induced enzyme. J Bacteriol. 1979;140:320\u20136.","journal-title":"J Bacteriol"},{"key":"591_CR27","first-page":"223","volume":"12","author":"WC Fuqua","year":"1992","unstructured":"Fuqua WC. An improved chloramphenicol resistance gene cassette for site-directed marker replacement mutagenesis. Biotechniques. 1992;12:223\u20135.","journal-title":"Biotechniques"},{"key":"591_CR28","volume-title":"Molecular cloning: A laboratory manual","author":"J Sambrook","year":"2001","unstructured":"Sambrook J, Rusell DW. Molecular cloning: A laboratory manual. New York USA: Cold Spring Harbor Laboratory Press; 2001."},{"issue":"6","key":"591_CR29","doi-asserted-by":"crossref","first-page":"e20425","DOI":"10.1371\/journal.pone.0020425","volume":"6","author":"F Uliczka","year":"2011","unstructured":"Uliczka F, Pisano F, Kochut A, Opitz W, Herbst K, Stolz T, et al. Monitoring of gene expression in bacteria during infections using an adaptable set of bioluminescent, fluorescent and colorigenic fusion vectors. PLoS One. 2011;6(6):e20425.","journal-title":"PLoS One"},{"key":"591_CR30","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1016\/j.biocel.2007.03.008","volume":"39","author":"L Egea","year":"2007","unstructured":"Egea L, Aguilera L, Gim\u00e9nez R, Sorolla MA, Aguilar J, Bad\u00eda J, et al. Role of secreted glyceraldehyde-3-phosphate dehydrogenase in the infection mechanism of enterohemorrhagic and enteropathogenic Escherichia coli: interaction of the extracellular enzyme with human plasminogen and fibrinogen. Int J Biochem Cell Biol. 2007;39:1190\u2013203.","journal-title":"Int J Biochem Cell Biol"},{"key":"591_CR31","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","volume":"193","author":"OH Lowry","year":"1951","unstructured":"Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin Phenol reagent. J Biol Chem. 1951;193:265\u201373.","journal-title":"J Biol Chem"},{"key":"591_CR32","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","volume":"222","author":"UK Laemmli","year":"1970","unstructured":"Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriphage T4. Nature. 1970;222:680\u20135.","journal-title":"Nature"},{"key":"591_CR33","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1002\/pmic.201300328","volume":"14","author":"L Aguilera","year":"2014","unstructured":"Aguilera L, Toloza L, Gim\u00e9nez R, Odena A, Oliveira E, Aguilar J, et al. Proteomic analysis of outer membrane vesicles from the probiotic strain Escherichia coli Nissle 1917. Proteomics. 2014;14:222\u20139.","journal-title":"Proteomics"},{"key":"591_CR34","doi-asserted-by":"crossref","first-page":"1557","DOI":"10.3945\/jn.109.120469","volume":"140","author":"F Roig-P\u00e9rez","year":"2010","unstructured":"Roig-P\u00e9rez F, Cortadellas N, Moret\u00f3 M, Ferrer R. Intracellular mechanisms involved in docosahexaenoic acid-induced increases in tight junction permeability in Caco-2 cell monolayers. J Nutr. 2010;140:1557\u201363.","journal-title":"J Nutr"},{"issue":"6","key":"591_CR35","doi-asserted-by":"crossref","first-page":"2465","DOI":"10.1128\/IAI.01494-08","volume":"77","author":"SM Harrington","year":"2009","unstructured":"Harrington SM, Sheikh J, Henderson IR, Ruiz-Perez F, Cohen PS, Nataro JP. The Pic protease of enteroaggregative Escherichia coli promotes intestinal colonization and growth in the presence of mucin. Infect Immun. 2009;77(6):2465\u201373.","journal-title":"Infect Immun"},{"issue":"7","key":"591_CR36","doi-asserted-by":"crossref","first-page":"2876","DOI":"10.1128\/IAI.00059-09","volume":"77","author":"MP Leatham","year":"2009","unstructured":"Leatham MP, Banerjee S, Autieri SM, Mercado-Lubo R, Conway T, Cohen PS. Precolonized human comensal Escherichia coli strains serve as a barrier to E. coli O157:H7 growth in the streptomycin-treated mouse intestine. Infect Immun. 2009;77(7):2876\u201386.","journal-title":"Infect Immun"},{"key":"591_CR37","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/S1286-4579(00)00272-0","volume":"2","author":"ME Konkel","year":"2000","unstructured":"Konkel ME, Tilly K. Temperature-regulated expression of bacterial virulence genes. Microbiol Infect. 2000;2:157\u201366.","journal-title":"Microbiol Infect"},{"key":"591_CR38","doi-asserted-by":"crossref","first-page":"34077","DOI":"10.1074\/jbc.M707352200","volume":"282","author":"N Duong","year":"2007","unstructured":"Duong N, Osborne S, Bustamante VH, Tomljenovic AM, Puente JL, Coombes BK. Thermosensing coordinates a cis-regulatory module for transcriptional activation of the intracellular virulence system in Salmonella enterica serovar Typhimurium. J Biol Chem. 2007;282:34077\u201384.","journal-title":"J Biol Chem"},{"key":"591_CR39","doi-asserted-by":"crossref","first-page":"e1002153","DOI":"10.1371\/journal.ppat.1002153","volume":"7","author":"HD Kamp","year":"2011","unstructured":"Kamp HD, Higgins DE. A protein thermometer controls temperature-dependent transcription of flagelar motility genes in Listeria monocytogenes. PLoS Pathog. 2011;7:e1002153.","journal-title":"PLoS Pathog"},{"key":"591_CR40","doi-asserted-by":"crossref","first-page":"3080","DOI":"10.1038\/ncomms4080","volume":"5","author":"D Munera","year":"2014","unstructured":"Munera D, Ritchie JM, Hatzios SH, Bronson R, Fang G, Schadt EE, et al. Autotransporters but not pAA are critical for rabbit colonization by Shiga toxin-producing Escherichia coli O104:H4. Nat Commun. 2014;5:3080.","journal-title":"Nat Commun"},{"issue":"6","key":"591_CR41","doi-asserted-by":"crossref","first-page":"2626","DOI":"10.1128\/IAI.01518-13","volume":"82","author":"V Vijayakumar","year":"2014","unstructured":"Vijayakumar V, Santiago A, Smith R, Smith M, Robins-Browne RM, Nataro JP. Role of class I serine protease autotransporter in the pathogenesis of Citrobacter reodentium colitis. Infect Immun. 2014;82(6):2626\u201336.","journal-title":"Infect Immun"},{"key":"591_CR42","doi-asserted-by":"crossref","first-page":"12881","DOI":"10.1073\/pnas.1101006108","volume":"108","author":"F Ruiz-Perez","year":"2011","unstructured":"Ruiz-Perez F, Wahid R, Fatherty CS, Kolappaswamy K, Rodriguez L, Santiago A, et al. Serine protease autotransporters fron Shigella flexneri and pathogenic Escherichia coli target a broad range of leukocyte glycoproteins. Proc Natl Acad Sci U S A. 2011;108:12881\u20136.","journal-title":"Proc Natl Acad Sci U S A"},{"key":"591_CR43","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1136\/gut.45.1.148","volume":"45","author":"M Salmi","year":"1999","unstructured":"Salmi M, Jalkanen S. Molecules controlling lymphocyte migration to the gut. Gut. 1999;45:148\u201353.","journal-title":"Gut"},{"issue":"2","key":"591_CR44","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1128\/IAI.01149-13","volume":"82","author":"J Adediran","year":"2014","unstructured":"Adediran J, Leatham-Jensen MP, Mokszycki ME, Frimodt-M\u00f8ller J, Krogfelt KA, Kazmierczak A, et al. An Escherichia coli Nissle 1917 missense mutant colonizes the streptomycin-treated mouse intestine better than the wild type but is not a better probiotic. Infect Immun. 2014;82(2):670\u201382.","journal-title":"Infect Immun"},{"issue":"2","key":"591_CR45","doi-asserted-by":"crossref","first-page":"294","DOI":"10.4269\/ajtmh.2009.80.294","volume":"80","author":"N Boisen","year":"2009","unstructured":"Boisen N, Ruiz-Perez F, Scheutz F, Krogfelt KA, Nataro JP. Short report: high prevalence of serine protease autotransporter cytotoxins among strains of enteroaggregative Escherichia coli. Am J Trp Med Hyg. 2009;80(2):294\u2013301.","journal-title":"Am J Trp Med Hyg"},{"issue":"2","key":"591_CR46","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1128\/JB.157.2.690-693.1984","volume":"157","author":"H Ochman","year":"1984","unstructured":"Ochman H, Selander RK. Standard reference strains of Escherichia coli from natural populations. J Bacteriol. 1984;157(2):690\u20133.","journal-title":"J Bacteriol"},{"issue":"10","key":"591_CR47","doi-asserted-by":"crossref","first-page":"4555","DOI":"10.1128\/AEM.66.10.4555-4558.2000","volume":"66","author":"O Clermont","year":"2000","unstructured":"Clermont O, Bonacorsi S, Bingen E. Rapid and simple determination of Escherichia coli phylogenetic group. Appl Environ Microbiol. 2000;66(10):4555\u20138.","journal-title":"Appl Environ Microbiol"},{"key":"591_CR48","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1038\/nm1734","volume":"14","author":"C Cirl","year":"2008","unstructured":"Cirl C, Wieser A, Yadav M, Duerr S, Schubert S, Fisher H, et al. Subversion of Toll-like receptor signaling by a unique family of bacterial Toll\/interleukin-1 receptor domain-containing proteins. Nat Med. 2008;14:399\u2013406.","journal-title":"Nat Med"},{"issue":"9","key":"591_CR49","doi-asserted-by":"crossref","first-page":"e1001120","DOI":"10.1371\/journal.ppat.1001120","volume":"6","author":"M Yadav","year":"2010","unstructured":"Yadav M, Zhang J, Fischer H, Huang W, Lutay N, Cirl C, et al. Inhibition of TIR domain signaling by TcpC: MyD88-dependent and independent effects on Escherichia coli virulence. PLoS Pathog. 2010;6(9):e1001120.","journal-title":"PLoS Pathog"}],"container-title":["BMC Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12866-015-0591-5","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,24]],"date-time":"2022-05-24T21:19:47Z","timestamp":1653427187000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.biomedcentral.com\/1471-2180\/15\/250"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,10,30]]},"references-count":49,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["591"],"URL":"https:\/\/doi.org\/10.1186\/s12866-015-0591-5","relation":{},"ISSN":["1471-2180"],"issn-type":[{"value":"1471-2180","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,10,30]]},"article-number":"250"}}