{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T14:13:52Z","timestamp":1770819232703,"version":"3.50.1"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"1","funder":[{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"crossref","award":["2010CB530204"],"award-info":[{"award-number":["2010CB530204"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31372443"],"award-info":[{"award-number":["31372443"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangdong Provincial S and T Project","award":["2014A020214003"],"award-info":[{"award-number":["2014A020214003"]}]},{"name":"Guangzhou Key Laboratory for Blood Safety","award":["201509010009"],"award-info":[{"award-number":["201509010009"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2017,12]]},"DOI":"10.1186\/s12866-017-1025-3","type":"journal-article","created":{"date-parts":[[2017,5,15]],"date-time":"2017-05-15T16:06:46Z","timestamp":1494864406000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Characterization of novel Omp31 antigenic epitopes of Brucella melitensis by monoclonal antibodies"],"prefix":"10.1186","volume":"17","author":[{"given":"Jinfeng","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feihuan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shouyi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peifang","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zuoping","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenjing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Pierre","family":"Allain","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2087-873X","authenticated-orcid":false,"given":"Chengyao","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,5,15]]},"reference":[{"key":"1025_CR1","doi-asserted-by":"crossref","first-page":"126","DOI":"10.3201\/eid2001.130324","volume":"20","author":"S Chen","year":"2014","unstructured":"Chen S, Zhang H, Liu X, Wang W, Hou S, Li T, Zhao S, Yang Z, Li C. Increasing threat of brucellosis to low-risk persons in urban settings, China. Emerg Infect Dis. 2014;20:126\u201330.","journal-title":"Emerg Infect Dis"},{"key":"1025_CR2","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/S0378-1135(02)00252-3","volume":"90","author":"S Deqiu","year":"2002","unstructured":"Deqiu S, Donglou X, Jiming Y. Epidemiology and control of brucellosis in China. Vet Microbiol. 2002;90:165\u201382.","journal-title":"Vet Microbiol"},{"key":"1025_CR3","doi-asserted-by":"crossref","first-page":"2001","DOI":"10.3201\/eid1612.091081","volume":"16","author":"WY Zhang","year":"2010","unstructured":"Zhang WY, Guo WD, Sun SH, Jiang JF, Sun HL, Li SL, Liu W, Cao WC. Human brucellosis, Inner Mongolia, China. Emerg Infect Dis. 2010;16:2001\u20133.","journal-title":"Emerg Infect Dis"},{"key":"1025_CR4","doi-asserted-by":"crossref","first-page":"3744","DOI":"10.1128\/IAI.64.9.3744-3751.1996","volume":"64","author":"N Vizca\u00edno","year":"1996","unstructured":"Vizca\u00edno N, Cloeckaert A, Zygmunt MS, Dubray G. Cloning, nucleotide sequence, and expression of the Brucella melitensis omp31 gene coding for an immunogenic major outer membrane protein. Infect Immun. 1996;64:3744\u201351.","journal-title":"Infect Immun"},{"key":"1025_CR5","first-page":"352493","volume":"2012","author":"ED Avila-Calder\u00f3n","year":"2012","unstructured":"Avila-Calder\u00f3n ED, Lopez-Merino A, Jain N, Peralta H, L\u00f3pez-Villegas EO, Sriranganathan N, Boyle SM, Witonsky S, Contreras-Rodr\u00edguez A. Characterization of outer membrane vesicles from Brucella melitensis and protection induced in mice. Clin Dev Immunol. 2012;2012:352493.","journal-title":"Clin Dev Immunol"},{"key":"1025_CR6","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1128\/CDLI.11.1.111-114.2004","volume":"11","author":"J Cassataro","year":"2004","unstructured":"Cassataro J, Pasquevich K, Bruno L, Wallach JC, Fossati CA, Baldi PC. Antibody reactivity to Omp31 from Brucella melitensis in human and animal infections by smooth and rough Brucellae. Clin Diagn Lab Immunol. 2004;11:111\u20134.","journal-title":"Clin Diagn Lab Immunol"},{"key":"1025_CR7","doi-asserted-by":"crossref","first-page":"8079","DOI":"10.1128\/IAI.73.12.8079-8088.2005","volume":"73","author":"J Cassataro","year":"2005","unstructured":"Cassataro J, Estein SM, Pasquevich KA, Velikovsky CA, de la Barrera S, Bowden R, Fossati CA, Giambartolomei GH. Vaccination with the recombinant Brucella outer membrane protein 31 or a derived 27-amino-acid synthetic peptide elicits a CD4+ T helper 1 response that protects against Brucella melitensis infection. Infect Immun. 2005;73:8079\u201388.","journal-title":"Infect Immun"},{"key":"1025_CR8","doi-asserted-by":"crossref","first-page":"6537","DOI":"10.1128\/IAI.73.10.6537-6546.2005","volume":"73","author":"J Cassataro","year":"2005","unstructured":"Cassataro J, Velikovsky CA, de la Barrera S, Estein SM, Bruno L, Bowden R, Pasquevich KA, Fossati CA, Giambartolomei GH. A DNA vaccine coding for the Brucella outer membrane protein 31 confers protection against B. melitensis and B. ovis infection by eliciting a specific cytotoxic response. Infect Immun. 2005;73:6537\u201346.","journal-title":"Infect Immun"},{"key":"1025_CR9","doi-asserted-by":"crossref","first-page":"6704","DOI":"10.1016\/j.vaccine.2009.08.097","volume":"27","author":"SM Estein","year":"2009","unstructured":"Estein SM, Fiorentino MA, Paolicchi FA, Clausse M, Manazza J, Cassataro J, Giambartolomei GH, Coria LM, Zylberman V, Fossati CA, Kjeken R, Goldbaum FA. The polymeric antigen BLSOmp31 confers protection against Brucella ovis infection in rams. Vaccine. 2009;27:6704\u201311.","journal-title":"Vaccine"},{"key":"1025_CR10","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.rvsc.2006.07.014","volume":"82","author":"VK Gupta","year":"2007","unstructured":"Gupta VK, Rout PK, Vihan VS. Induction of immune response in mice with a DNA vaccine encoding outer membrane protein (omp31) of Brucella melitensis 16M. Res Vet Sci. 2007;82:305\u201313.","journal-title":"Res Vet Sci"},{"key":"1025_CR11","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1128\/CVI.00111-13","volume":"20","author":"S Tiwari","year":"2013","unstructured":"Tiwari S, Kumar A, Thavaselvam D, Mangalgi S, Rathod V, Prakash A, Barua A, Arora S, Sathyaseelan K. Development and comparative evaluation of a plate enzyme-linked immunosorbent assay based on recombinant outer membrane antigens Omp28 and Omp31 for diagnosis of human brucellosis. Clin Vaccine Immunol. 2013;20:1217\u201322.","journal-title":"Clin Vaccine Immunol"},{"key":"1025_CR12","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1016\/j.vaccine.2013.12.028","volume":"32","author":"W Wang","year":"2014","unstructured":"Wang W, Wu J, Qiao J, Weng Y, Zhang H, Liao Q, Qiu J, Chen C, Allain JP, Li C. Evaluation of humoral and cellular immune responses to BP26 and OMP31 epitopes in the attenuated Brucella melitensis vaccinated sheep. Vaccine. 2014;32:825\u201333.","journal-title":"Vaccine"},{"key":"1025_CR13","doi-asserted-by":"crossref","first-page":"3980","DOI":"10.1128\/IAI.58.12.3980-3987.1990","volume":"58","author":"A Cloeckaert","year":"1990","unstructured":"Cloeckaert A, de Wergifosse P, Dubray G, Limet JN. Identification of seven surface-exposed Brucella outer membrane proteins by use of monoclonal antibodies: immunogold labeling for electron microscopy and enzyme-linked immunosorbent assay. Infect Immun. 1990;58:3980\u20137.","journal-title":"Infect Immun"},{"key":"1025_CR14","doi-asserted-by":"crossref","first-page":"7020","DOI":"10.1128\/IAI.69.11.7020-7028.2001","volume":"69","author":"N Vizca\u00edno","year":"2001","unstructured":"Vizca\u00edno N, Kittelberger R, Cloeckaert A, Mar\u00edn CM, Fern\u00e1ndez-Lago L. Minor nucleotide substitutions in the omp31 gene of Brucella ovis result in antigenic differences in the major outer membrane protein that it encodes compared to those of the other Brucella species. Infect Immun. 2001;69:7020\u20138.","journal-title":"Infect Immun"},{"key":"1025_CR15","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.vetmic.2004.05.004","volume":"102","author":"SM Estein","year":"2004","unstructured":"Estein SM, Cheves PC, Fiorentino MA, Cassataro J, Paolicchi FA, Bowden RA. Immunogenicity of recombinant Omp31 from Brucella melitensis in rams and serum bactericidal activity against B. ovis. Vet Microbiol. 2004;102:203\u201313.","journal-title":"Vet Microbiol"},{"key":"1025_CR16","volume":"7","author":"J Qiu","year":"2012","unstructured":"Qiu J, Wang W, Wu J, Zhang H, Wang Y, Qiao J, Chen C, Gao GF, Allain JP, Li C. Characterization of periplasmic protein BP26 epitopes of Brucella melitensis reacting with murine monoclonal and sheep antibodies. PLoS One. 2012;7:e34246.","journal-title":"PLoS One"},{"key":"1025_CR17","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1038\/383787a0","volume":"383","author":"AK Abbas","year":"1996","unstructured":"Abbas AK, Murphy KM, Sher A. Functional diversity of helper T lymphocytes. Nature. 1996;383:787\u201393.","journal-title":"Nature"},{"key":"1025_CR18","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1089\/aid.2009.0223","volume":"26","author":"K Banerjee","year":"2010","unstructured":"Banerjee K, Klasse PJ, Sanders RW, Pereyra F, Michael E, Lu M, Walker BD, Moore JP. IgG subclass profiles in infected HIV type 1 controllers and chronic progressors and in uninfected recipients of Env vaccines. AIDS Res Hum Retrovir. 2010;26:445\u201358.","journal-title":"AIDS Res Hum Retrovir"},{"key":"1025_CR19","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/1479-5876-10-4","volume":"10","author":"ML Visciano","year":"2012","unstructured":"Visciano ML, Tagliamonte M, Tornesello ML, Buonaguro FM, Buonaguro L. Effects of adjuvants on IgG subclasses elicited by virus-like particles. J Transl Med. 2012;10:4.","journal-title":"J Transl Med"},{"key":"1025_CR20","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1615\/CritRevImmunol.v26.i5.30","volume":"26","author":"CL Baldwin","year":"2006","unstructured":"Baldwin CL, Goenka R. Host immune responses to the intracellular bacteria Brucella, does the bacteria instruct the host to facilitate chronic infection? Crit Rev Immunol. 2006;26:407\u201342.","journal-title":"Crit Rev Immunol"},{"key":"1025_CR21","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1128\/IAI.00994-09","volume":"78","author":"MA Durward","year":"2010","unstructured":"Durward MA, Harms J, Magnani DM, Eskra L, Splitter GA. Discordant Brucella melitensis antigens yield cognate CD8+ T cells in vivo. Infect Immun. 2010;78:168\u201376.","journal-title":"Infect Immun"},{"key":"1025_CR22","doi-asserted-by":"crossref","first-page":"4017","DOI":"10.1016\/j.vaccine.2012.04.036","volume":"30","author":"VK Gupta","year":"2012","unstructured":"Gupta VK, Radhakrishnan G, Harms J, Splitter G. Invasive Escherichia coli vaccines expressing Brucella melitensis outer membrane proteins 31 or 16 or periplasmic protein BP26 confer protection in mice challenged with B. melitensis. Vaccine. 2012;30:4017\u201322.","journal-title":"Vaccine"},{"key":"1025_CR23","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1128\/IAI.01151-08","volume":"77","author":"KA Pasquevich","year":"2009","unstructured":"Pasquevich KA, Estein SM, Garc\u00eda Samartino C, Zwerdling A, Coria LM, Barrionuevo P, Fossati CA, Giambartolomei GH, Cassataro J. Immunization with recombinant Brucella species outer membrane protein Omp16 or Omp19 in adjuvant induces specific CD4+ and CD8+ T cells as well as systemic and oral protection against Brucella abortus infection. Infect Immun. 2009;77:436\u201345.","journal-title":"Infect Immun"},{"key":"1025_CR24","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1016\/S1286-4579(00)00317-8","volume":"2","author":"RA Bowden","year":"2000","unstructured":"Bowden RA, Estein SM, Zygmunt MS, Dubray G, Cloeckaert A. Identification of protective outer membrane antigens of Brucella ovis by passive immunization of mice with monoclonal antibodies. Microbes Infect. 2000;2:481\u20138.","journal-title":"Microbes Infect"},{"key":"1025_CR25","doi-asserted-by":"crossref","first-page":"3945","DOI":"10.1128\/IAI.63.10.3945-3952.1995","volume":"63","author":"RA Bowden","year":"1995","unstructured":"Bowden RA, Cloeckaert A, Zygmunt MS, Bernard S, Dubray G. Surface exposure of outer membrane protein and lipopolysaccharide epitopes in Brucella species studied by enzyme-linked immunosorbent assay and flow cytometry. Infect Immun. 1995;63:3945\u201352.","journal-title":"Infect Immun"},{"key":"1025_CR26","doi-asserted-by":"crossref","first-page":"4437","DOI":"10.1016\/j.vaccine.2007.03.028","volume":"25","author":"J Cassataro","year":"2007","unstructured":"Cassataro J, Pasquevich KA, Estein SM, Laplagne DA, Velikovsky CA, de la Barrera S, Bowden R, Fossati CA, Giambartolomei GH, Goldbaum FA. A recombinant subunit vaccine based on the insertion of 27 amino acids from Omp31 to the N-terminus of BLS induced a similar degree of protection against B. ovis than rev.1 vaccination. Vaccine. 2007;25:4437\u201346.","journal-title":"Vaccine"},{"key":"1025_CR27","doi-asserted-by":"crossref","first-page":"e2926","DOI":"10.1371\/journal.pntd.0002926","volume":"8","author":"KP Patra","year":"2014","unstructured":"Patra KP, Saito M, Atluri VL, Rol\u00e1n HG, Young B, Kerrinnes T, Smits H, Ricaldi JN, Gotuzzo E, Gilman RH, Tsolis RM, Vinetz JM. A protein-conjugate approach to develop a monoclonal antibody-based antigen detection test for the diagnosis of human brucellosis. PLoS Negl Trop Dis. 2014;8:e2926.","journal-title":"PLoS Negl Trop Dis"},{"key":"1025_CR28","doi-asserted-by":"crossref","first-page":"2084","DOI":"10.1128\/JCM.02159-08","volume":"47","author":"MJ Casta\u00f1o","year":"2009","unstructured":"Casta\u00f1o MJ, Solera J. Chronic brucellosis and persistence of Brucella melitensis DNA. J Clin Microbiol. 2009;47:2084\u20139.","journal-title":"J Clin Microbiol"},{"key":"1025_CR29","doi-asserted-by":"crossref","first-page":"e70214","DOI":"10.1371\/journal.pone.0070214","volume":"8","author":"Y Bian","year":"2013","unstructured":"Bian Y, Zhao S, Zhu S, Zeng J, Li T, Fu Y, Wang Y, Zheng X, Zhang L, Wang W, Yang B, Zhou Y, Allain JP, Li C. Significance of monoclonal antibodies against the conserved epitopes within non-structural protein 3 helicase of hepatitis C virus. PLoS One. 2013;8:e70214.","journal-title":"PLoS One"},{"key":"1025_CR30","doi-asserted-by":"crossref","first-page":"e42455","DOI":"10.1371\/journal.pone.0042455","volume":"7","author":"L Zhang","year":"2012","unstructured":"Zhang L, Yin S, Tan W, Xiao D, Weng Y, Wang W, Li T, Shi J, Shuai L, Li H, Zhou J, Allain JP, Li C. Recombinant interferon-\u03b3 lentivirus co-infection inhibits adenovirus replication ex vivo. PLoS One. 2012;7:e42455.","journal-title":"PLoS One"}],"container-title":["BMC Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12866-017-1025-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T19:32:21Z","timestamp":1602099141000},"score":1,"resource":{"primary":{"URL":"http:\/\/bmcmicrobiol.biomedcentral.com\/articles\/10.1186\/s12866-017-1025-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,5,15]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["1025"],"URL":"https:\/\/doi.org\/10.1186\/s12866-017-1025-3","relation":{},"ISSN":["1471-2180"],"issn-type":[{"value":"1471-2180","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5,15]]},"article-number":"115"}}