{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T03:19:38Z","timestamp":1791688778108,"version":"4.3.4"},"reference-count":21,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2018,5,31]],"date-time":"2018-05-31T00:00:00Z","timestamp":1527724800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["K2015-57X-03163-43-4"],"award-info":[{"award-number":["K2015-57X-03163-43-4"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005753","name":"Kungliga Fysiografiska S\u00e4llskapet i Lund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005753","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2018,12]]},"DOI":"10.1186\/s12866-018-1196-6","type":"journal-article","created":{"date-parts":[[2018,5,31]],"date-time":"2018-05-31T13:41:27Z","timestamp":1527774087000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["A novel PBP3 substitution in Haemophilus influenzae confers reduced aminopenicillin susceptibility"],"prefix":"10.1186","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3884-0668","authenticated-orcid":false,"given":"John","family":"Thegerstr\u00f6m","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika","family":"Matuschek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Ching","family":"Su","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristian","family":"Riesbeck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fredrik","family":"Resman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,5,31]]},"reference":[{"issue":"2","key":"1196_CR1","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1128\/CMR.00040-06","volume":"20","author":"S Tristram","year":"2007","unstructured":"Tristram S, Jacobs MR, Appelbaum PC. Antimicrobial resistance in Haemophilus influenzae. Clin Microbiol Rev. 2007;20(2):368\u201389.","journal-title":"Clin Microbiol Rev"},{"issue":"5","key":"1196_CR2","doi-asserted-by":"publisher","first-page":"1630","DOI":"10.1128\/AAC.48.5.1630-1639.2004","volume":"48","author":"FS Kaczmarek","year":"2004","unstructured":"Kaczmarek FS, Gootz TD, Dib-Hajj F, Shang W, Hallowell S, Cronan M. Genetic and molecular characterization of beta-lactamase-negative ampicillin-resistant Haemophilus influenzae with unusually high resistance to ampicillin. Antimicrob Agents Chemother. 2004;48(5):1630\u20139.","journal-title":"Antimicrob Agents Chemother"},{"issue":"6","key":"1196_CR3","doi-asserted-by":"publisher","first-page":"1693","DOI":"10.1128\/AAC.45.6.1693-1699.2001","volume":"45","author":"K Ubukata","year":"2001","unstructured":"Ubukata K, Shibasaki Y, Yamamoto K, Chiba N, Hasegawa K, Takeuchi Y, Sunakawa K, Inoue M, Konno M. Association of amino acid substitutions in penicillin-binding protein 3 with beta-lactam resistance in beta-lactamase-negative ampicillin-resistant Haemophilus influenzae. Antimicrob Agents Chemother. 2001;45(6):1693\u20139.","journal-title":"Antimicrob Agents Chemother"},{"issue":"11","key":"1196_CR4","doi-asserted-by":"publisher","first-page":"3969","DOI":"10.1128\/AAC.00422-07","volume":"51","author":"M Hotomi","year":"2007","unstructured":"Hotomi M, Fujihara K, Billal DS, Suzuki K, Nishimura T, Baba S, Yamanaka N. Genetic characteristics and clonal dissemination of beta-lactamase-negative ampicillin-resistant Haemophilus influenzae strains isolated from the upper respiratory tract of patients in Japan. Antimicrob Agents Chemother. 2007;51(11):3969\u201376.","journal-title":"Antimicrob Agents Chemother"},{"issue":"7","key":"1196_CR5","doi-asserted-by":"publisher","first-page":"2564","DOI":"10.1128\/AAC.00354-07","volume":"51","author":"S Garcia-Cobos","year":"2007","unstructured":"Garcia-Cobos S, Campos J, Lazaro E, Roman F, Cercenado E, Garcia-Rey C, Perez-Vazquez M, Oteo J, de Abajo F. Ampicillin-resistant non-beta-lactamase-producing Haemophilus influenzae in Spain: recent emergence of clonal isolates with increased resistance to cefotaxime and cefixime. Antimicrob Agents Chemother. 2007;51(7):2564\u201373.","journal-title":"Antimicrob Agents Chemother"},{"issue":"7","key":"1196_CR6","doi-asserted-by":"publisher","first-page":"2208","DOI":"10.1128\/AAC.46.7.2208-2218.2002","volume":"46","author":"H Dabernat","year":"2002","unstructured":"Dabernat H, Delmas C, Seguy M, Pelissier R, Faucon G, Bennamani S, Pasquier C. Diversity of beta-lactam resistance-conferring amino acid substitutions in penicillin-binding protein 3 of Haemophilus influenzae. Antimicrob Agents Chemother. 2002;46(7):2208\u201318.","journal-title":"Antimicrob Agents Chemother"},{"issue":"8","key":"1196_CR7","doi-asserted-by":"publisher","first-page":"1117","DOI":"10.1111\/j.1469-0691.2009.03052.x","volume":"16","author":"D Skaare","year":"2010","unstructured":"Skaare D, Allum AG, Anthonisen IL, Jenkins A, Lia A, Strand L, Tveten Y, Kristiansen BE. Mutant ftsI genes in the emergence of penicillin-binding protein-mediated beta-lactam resistance in Haemophilus influenzae in Norway. Clin Microbiol Infec. 2010;16(8):1117\u201324.","journal-title":"Clin Microbiol Infec"},{"issue":"11","key":"1196_CR8","doi-asserted-by":"publisher","first-page":"3589","DOI":"10.1128\/JCM.01630-15","volume":"53","author":"D Skaare","year":"2015","unstructured":"Skaare D, Lia A, Hannisdal A, Tveten Y, Matuschek E, Kahlmeter G, Kristiansen BE. Haemophilus influenzae with non-Beta-lactamase-mediated Beta-lactam resistance: easy to find but hard to categorize. J Clin Microbiol. 2015;53(11):3589\u201395.","journal-title":"J Clin Microbiol"},{"issue":"7","key":"1196_CR9","doi-asserted-by":"publisher","first-page":"2834","DOI":"10.1128\/AAC.49.7.2834-2839.2005","volume":"49","author":"Y Osaki","year":"2005","unstructured":"Osaki Y, Sanbongi Y, Ishikawa M, Kataoka H, Suzuki T, Maeda K, Ida T. Genetic approach to study the relationship between penicillin-binding protein 3 mutations and Haemophilus influenzae beta-lactam resistance by using site-directed mutagenesis and gene recombinants. Antimicrob Agents Chemother. 2005;49(7):2834\u20139.","journal-title":"Antimicrob Agents Chemother"},{"key":"1196_CR10","unstructured":"The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. Version. 8:0. http:\/\/www.eucast.org\/"},{"issue":"6","key":"1196_CR11","doi-asserted-by":"publisher","first-page":"1401","DOI":"10.1093\/jac\/dks070","volume":"67","author":"A Sondergaard","year":"2012","unstructured":"Sondergaard A, Petersen MT, Fuursted K, Norskov-Lauritsen N. Detection of N526K-substituted penicillin-binding protein 3 conferring low-level mutational resistance to beta-lactam antibiotics in Haemophilus influenzae by disc diffusion testing on Mueller-Hinton agar according to EUCAST guidelines. The J Antimicrob Chemother. 2012;67(6):1401\u20134.","journal-title":"The J Antimicrob Chemother"},{"issue":"9","key":"1196_CR12","doi-asserted-by":"publisher","first-page":"1979","DOI":"10.1128\/AAC.41.9.1979","volume":"41","author":"A Melhus","year":"1997","unstructured":"Melhus A, Janson H, Westman E, Hermansson A, Forsgren A, Prellner K. Amoxicillin treatment of experimental acute otitis media caused by Haemophilus influenzae with non-beta-lactamase-mediated resistance to beta-lactams: aspects of virulence and treatment. Antimicrob Agents Chemother. 1997;41(9):1979\u201384.","journal-title":"Antimicrob Agents Chemother"},{"key":"1196_CR13","first-page":"57","volume":"71","author":"G Poje","year":"2003","unstructured":"Poje G, Redfield RJ. Transformation of Haemophilus influenzae. Methods in molecular medicine. 2003;71:57\u201370.","journal-title":"Methods in molecular medicine"},{"key":"1196_CR14","unstructured":"International Standards Organisation. Reference method for testing the in vitro activity of antimicrobial agents against rapidly growing aerobic bacteria involved in infectious diseases. ISO. 1996:20776\u20131."},{"key":"1196_CR15","unstructured":"The European Committee on Antimicrobial Susceptibility Testing. Media preparation for EUCAST disk diffusion testing and for determination of MIC values by the broth microdilution method. v 4.0, 2014. http:\/\/www.eucast.org\/ , last Accessed 03 Mar 2017."},{"issue":"4","key":"1196_CR16","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1128\/AAC.1.4.283","volume":"1","author":"CH O'Callaghan","year":"1972","unstructured":"O'Callaghan CH, Morris A, Kirby SM, Shingler AH. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972;1(4):283\u20138.","journal-title":"Antimicrob Agents Chemother"},{"key":"1196_CR17","doi-asserted-by":"crossref","unstructured":"Karlsson C VA, Matuschek E, Skaare D and Kahlmeter G. : Evaluation of Etest, M.I.C.E. and MIC Test Strip beta-lactam gradient tests for beta-lactamase negative Haemophilus influenzae with beta-lactam resistance due to PBP3 substitutions. 26th ECCMID, Amsterdam, the Netherlands, 2016.","DOI":"10.26226\/morressier.56d6be76d462b80296c97794"},{"issue":"4","key":"1196_CR18","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1038\/nrmicro2319","volume":"8","author":"DI Andersson","year":"2010","unstructured":"Andersson DI, Hughes D. Antibiotic resistance and its cost: is it possible to reverse resistance? Nat Rev Microbiol. 2010;8(4):260\u201371.","journal-title":"Nat Rev Microbiol"},{"issue":"3","key":"1196_CR19","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1093\/jac\/dkg107","volume":"51","author":"K Straker","year":"2003","unstructured":"Straker K, Wootton M, Simm AM, Bennett PM, MacGowan AP, Walsh TR. Cefuroxime resistance in non-beta-lactamase Haemophilus influenzae is linked to mutations in ftsI. J Antimicrob Chemother. 2003;51(3):523\u201330.","journal-title":"J Antimicrob Chemother"},{"issue":"8","key":"1196_CR20","doi-asserted-by":"publisher","first-page":"4408","DOI":"10.1128\/AAC.00415-12","volume":"56","author":"F Resman","year":"2012","unstructured":"Resman F, Ristovski M, Forsgren A, Kaijser B, Kronvall G, Medstrand P, Melander E, Odenholt I, Riesbeck K. Increase of beta-lactam-resistant invasive Haemophilus influenzae in Sweden, 1997 to 2010. Antimicrob Agents Chemother. 2012;56(8):4408\u201315.","journal-title":"Antimicrob Agents Chemother"},{"issue":"10","key":"1196_CR21","doi-asserted-by":"publisher","first-page":"2909","DOI":"10.1093\/jac\/dkw226","volume":"71","author":"F de Velde","year":"2016","unstructured":"de Velde F, de Winter BC, Koch BC, van Gelder T, Mouton JW. Non-linear absorption pharmacokinetics of amoxicillin: consequences for dosing regimens and clinical breakpoints. J Antimicrob Chemother. 2016;71(10):2909\u201317.","journal-title":"J Antimicrob Chemother"}],"container-title":["BMC Microbiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12866-018-1196-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1186\/s12866-018-1196-6\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1186\/s12866-018-1196-6.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,2]],"date-time":"2020-11-02T22:55:17Z","timestamp":1604357717000},"score":1,"resource":{"primary":{"URL":"https:\/\/bmcmicrobiol.biomedcentral.com\/articles\/10.1186\/s12866-018-1196-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5,31]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,12]]}},"alternative-id":["1196"],"URL":"https:\/\/doi.org\/10.1186\/s12866-018-1196-6","relation":{},"ISSN":["1471-2180"],"issn-type":[{"value":"1471-2180","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5,31]]},"assertion":[{"value":"5 January 2018","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 May 2018","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 May 2018","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Not applicable. Only microorganisms and no human material were handled as part of this project.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The authors declare that they have no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}},{"value":"Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Publisher\u2019s Note"}}],"article-number":"48"}}