{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T16:10:04Z","timestamp":1777651804963,"version":"3.51.4"},"reference-count":32,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2019,11,14]],"date-time":"2019-11-14T00:00:00Z","timestamp":1573689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,11,14]],"date-time":"2019-11-14T00:00:00Z","timestamp":1573689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Austrian Science Fund","award":["P_31507"],"award-info":[{"award-number":["P_31507"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["BMC Microbiol"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>High temperature requirement A (HtrA) is a widely expressed chaperone and serine protease in bacteria. HtrA proteases assemble and hydrolyze misfolded proteins to enhance bacterial survival under stress conditions. <jats:italic>Listeria monocytogenes<\/jats:italic> (<jats:italic>L. monocytogenes<\/jats:italic>) is a foodborne pathogen that induces listeriosis in humans. In previous studies, it was shown that deletion of <jats:italic>htrA<\/jats:italic> in the genome of <jats:italic>L. monocytogenes<\/jats:italic> increased the susceptibility to cellular stress and attenuated virulence. However, expression and protease activity of listerial HtrA (LmHtrA) were never analyzed in detail.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>In this study, we cloned LmHtrA wildtype (LmHtrA<jats:sup>wt<\/jats:sup>) and generated a proteolytic inactive LmHtrA<jats:sup>SA<\/jats:sup> mutant. Recombinant LmHtrA<jats:sup>wt<\/jats:sup> and LmHtrA<jats:sup>SA<\/jats:sup> were purified and the proteolytic activity was analyzed in casein zymography and in vitro cleavage assays. LmHtrA activity could be efficiently blocked by a small molecule inhibitor targeting bacterial HtrA proteases. The expression of LmHtrA was enhanced in the stationary growth phase of <jats:italic>L. monocytogenes<\/jats:italic> and significantly contributed to bacterial survival at high temperatures.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Our data show that LmHtrA is a highly active caseinolytic protease and provide a deeper insight into the function and mechanism, which could lead to medical and biotechnological applications in the future.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-019-1633-1","type":"journal-article","created":{"date-parts":[[2019,11,14]],"date-time":"2019-11-14T16:02:49Z","timestamp":1573747369000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The proteolytic activity of Listeria monocytogenes HtrA"],"prefix":"10.1186","volume":"19","author":[{"given":"Carmen M.","family":"Abfalter","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"Bernegger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miroslaw","family":"Jarzab","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gernot","family":"Posselt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karthe","family":"Ponnuraj","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7011-6162","authenticated-orcid":false,"given":"Silja","family":"Wessler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,11,14]]},"reference":[{"key":"1633_CR1","doi-asserted-by":"crossref","unstructured":"Davis ML, Ricke SC, Donaldson JR: Establishment of Listeria monocytogenes in the Gastrointestinal Tract. 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