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Increasing genomic and transcriptomic resources and recent advances in oomycete biology demand new strategies for genetic modification of oomycetes. Most oomycete transformation procedures rely on geneticin-based selection of transgenic strains.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>We established N-acetyltransferase AAC(3)-I as a gentamicin-based selectable marker for oomycete transformation without interference with existing geneticin resistance. Strains carrying gentamicin resistance are fully infectious in plants. We further demonstrate the usefulness of this new antibiotic selection to super-transform well-characterized, already fluorescently-labelled <jats:italic>P. palmivora<\/jats:italic> strains and provide a comprehensive protocol for maintenance and zoospore electro-transformation of <jats:italic>Phytophthora<\/jats:italic> strains to aid in plant-pathogen research.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>N-acetyltransferase AAC(3)-I is functional in <jats:italic>Phytophthora<\/jats:italic> oomycetes. In addition, the substrate specificity of the AAC(3)-I enzyme allows for re-transformation of geneticin-resistant strains. Our findings and resources widen the possibilities to study oomycete cell biology and plant-oomycete interactions.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-019-1642-0","type":"journal-article","created":{"date-parts":[[2019,11,27]],"date-time":"2019-11-27T15:03:40Z","timestamp":1574867020000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["N-acetyltransferase AAC(3)-I confers gentamicin resistance to Phytophthora palmivora and Phytophthora infestans"],"prefix":"10.1186","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7218-7850","authenticated-orcid":false,"given":"Edouard","family":"Evangelisti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9936-4354","authenticated-orcid":false,"given":"Temur","family":"Yunusov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1961-4187","authenticated-orcid":false,"given":"Liron","family":"Shenhav","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7836-5881","authenticated-orcid":false,"given":"Sebastian","family":"Schornack","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,11,27]]},"reference":[{"key":"1642_CR1","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1016\/j.tim.2007.10.013","volume":"16","author":"AJ Phillips","year":"2008","unstructured":"Phillips AJ, Anderson VL, Robertson EJ, Secombes CJ, van West P. 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