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However, only limited studies have focused on the gut microbiota of freshwater snails. <jats:italic>Pomacea canaliculata<\/jats:italic> is considered one of the worst invasive alien species in the world. Elucidating the diversity and composition of the microbiota in the gut of <jats:italic>P. canaliculata<\/jats:italic> snails may be helpful for better understanding the widespread invasion of this snail species. In this study, the buccal masses, stomachs, and intestines were isolated from seven <jats:italic>P. canaliculata<\/jats:italic> snails. The diversity and composition of the microbiota in the three gut sections were then investigated based on high-throughput Illumina sequencing targeting the V3-V4 regions of the <jats:italic>16S<\/jats:italic> rRNA gene.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>The diversity of the microbiota was highest in the intestine but lowest in the buccal mass. A total of 29 phyla and 111 genera of bacteria were identified in all of the samples. In general, <jats:italic>Ochrobactrum<\/jats:italic>, a genus of putative cellulose-degrading bacteria, was the most abundant (overall relative abundance: 13.6%), followed by <jats:italic>Sediminibacterium<\/jats:italic> (9.7%), <jats:italic>Desulfovibrio<\/jats:italic> (7.8%), an unclassified genus in the family Aeromonadaceae (5.4%), and <jats:italic>Cloacibacterium<\/jats:italic> (5.4%). The composition of the microbiota was diverse among the different gut sections. <jats:italic>Ochrobactrum<\/jats:italic> (relative abundance: 23.15%\u2009\u00b1\u20097.92%) and <jats:italic>Sediminibacterium<\/jats:italic> (16.95\u2009\u00b1\u20095.70%) were most abundant in the stomach, an unclassified genus in the family Porphyromonadaceae (14.28\u2009\u00b1\u20097.29%) and <jats:italic>Leptotrichia<\/jats:italic> (8.70\u2009\u00b1\u20094.46%) were highest in the buccal mass, and two genera in the families Aeromonadaceae (7.55\u2009\u00b1\u20094.53%) and Mollicutes (13.47\u2009\u00b1\u200913.03%) were highest in the intestine.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>The diversity and composition of the microbiome vary among different gut sections of <jats:italic>P. canaliculata<\/jats:italic> snails. Putative cellulose-degrading bacteria are enriched in the gut of <jats:italic>P. canaliculata<\/jats:italic>.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12866-019-1661-x","type":"journal-article","created":{"date-parts":[[2019,12,5]],"date-time":"2019-12-05T15:02:49Z","timestamp":1575558169000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Spatial structure of the microbiome in the gut of Pomacea canaliculata"],"prefix":"10.1186","volume":"19","author":[{"given":"Lan-Hua","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan","family":"Lv","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ding-Qi","family":"Bi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun-Hai","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia-Tong","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi-Yuan","family":"Yue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guang-Yao","family":"Mao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhong-Xin","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun-Feng","family":"Tang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,12,5]]},"reference":[{"issue":"10","key":"1661_CR1","doi-asserted-by":"publisher","first-page":"2965","DOI":"10.1890\/09-1213.1","volume":"91","author":"MP Carey","year":"2010","unstructured":"Carey MP, Wahl DH. 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Collection of the <i>P. canaliculata<\/i> snails from Nanheng River was permitted by Qingpu Center for Disease Control and Prevention.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare that they have no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"273"}}