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To the best of our knowledge, a large proportion of iron in the human body is accumulated and stored in the liver. We hypothesize that increased iron availability is an important factor driving liver abscess formation and we therefore aim to understand the effects of iron on\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      causing liver abscesses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      All tested\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      clinical isolates, including those isolated from liver abscesses and other abdominal invasive infection sites, grew optimally when cultured in LB broth supplemented with 50\u2009\u03bcM iron and exhibited the strongest biofilm formation ability under those conditions. Decreased growth and biofilm formation ability were observed in all tested strains when cultured with an iron chelator (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05). The infection model of\n                      <jats:italic>G. mellonella<\/jats:italic>\n                      larvae indicated the virulence of liver abscess-causing\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      (2\/3) cultured in LB broth with additional iron was significantly higher than those under iron-restricted conditions (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05). The relative expression levels of the four siderophore genes (\n                      <jats:italic>iucB<\/jats:italic>\n                      ,\n                      <jats:italic>iroB<\/jats:italic>\n                      ,\n                      <jats:italic>irp1<\/jats:italic>\n                      ,\n                      <jats:italic>entB<\/jats:italic>\n                      ) in\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      strains isolated from liver abscesses cultured with additional iron were lower than those under iron-restricted conditions (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009&lt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      It is suggested by our research that iron in the environment can promote growth, biofilm formation and enhance virulence of\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      causing liver abscesses\n                      <jats:italic>.<\/jats:italic>\n                      A lower expression of siderophore genes correlates with increased virulence of liver abscess-causing\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      . Further deeper evaluation of these phenomena is warranted.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12866-020-01727-5","type":"journal-article","created":{"date-parts":[[2020,2,18]],"date-time":"2020-02-18T04:02:54Z","timestamp":1581998574000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":77,"title":["Effects of iron on the growth, biofilm formation and virulence of Klebsiella pneumoniae causing liver abscess"],"prefix":"10.1186","volume":"20","author":[{"given":"Tao","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guofeng","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siqin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiucai","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajie","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianming","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tieli","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,2,18]]},"reference":[{"issue":"3","key":"1727_CR1","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1128\/MMBR.00078-15","volume":"80","author":"MK Paczosa","year":"2016","unstructured":"Paczosa MK, Mecsas J. 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