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It is a fatal disease without early diagnosis and treatment; thus, the availability of affordable diagnostic tools is crucial. However, due to delays caused by import regulations, procurement and late delivery of imported test kits, accessibility remains a problem in the control program. Therefore, we aimed to produce and evaluate the performance of an in-house liquid (AQ) direct agglutination test (DAT) antigen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p>The AQ-DAT was produced at the Armauer Hansen Research Institute, using<jats:italic>Leishmania donovani<\/jats:italic>strain (MHOM\/ET\/67\/L82<jats:italic>)<\/jats:italic>. Sera from 272 participants; 110 microscopically confirmed cases of VL, 76 apparently healthy and 86 patients who had infectious disease other than VL were tested with AQ-DAT, and standard kits: Freeze-dried DAT (FD-DAT) and rK39. Taking microscopy as a gold standard; the sensitivity and specificity of the AQ-DAT were 97.3 and 98.8%, respectively. It had high degrees of agreement (k\u2009&gt;\u20090.8), with a significant (<jats:italic>P<\/jats:italic>\u00a0&lt;\u20090.05) correlation compared to microscopy, FD-DAT, and rK39.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Although further standardization is required, the in-house AQ-DAT could improve diagnostic accessibility, minimize intermittent stock outs and strengthen the national VL control program.<\/jats:p><\/jats:sec>","DOI":"10.1186\/s12866-020-01780-0","type":"journal-article","created":{"date-parts":[[2020,4,15]],"date-time":"2020-04-15T10:03:05Z","timestamp":1586944985000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Validation of in-house liquid direct agglutination test antigen: the potential diagnostic test in visceral Leishimaniasis endemic areas of Northwest Ethiopia"],"prefix":"10.1186","volume":"20","author":[{"given":"Birhanu","family":"Ayelign","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammedamin","family":"Jemal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markos","family":"Negash","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meaza","family":"Genetu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadelo","family":"Wondmagegn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayalew Jejaw","family":"Zeleke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ligabaw","family":"Worku","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abebe Genetu","family":"Bayih","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Girma","family":"Shumie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sinknesh Wolde","family":"Behaksra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiruwork","family":"Fenta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Demekech","family":"Damte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arega","family":"Yeshanew","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Endalamaw","family":"Gadisa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,4,15]]},"reference":[{"key":"1780_CR1","doi-asserted-by":"crossref","unstructured":"Bsrat A, Berhe M, Gadissa E, Taddele H, Tekle Y, Hagos Y et.al. 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Each study participant was informed about the objective of the study and written informed consent was obtained from each participant before they were enrolled and asked to give sample. Written informed consent was also obtained from the legal guardian\/parent on behalf of the child \/ young person. The legal guardian\/parent have been informed of the right to withdraw the child \/ young person from the study at any time. Assent was asked for children participants under the age of 16\u2009years in addition to their parents\/guardian consent. All confirmed cases were linked to the appropriate department for proper treatment and followed-up as per the guideline of the UoGH.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declared no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"90"}}