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Our research group identified a low expression of <jats:italic>lipF<\/jats:italic> gene in <jats:italic>Mycobacterium tuberculosis<\/jats:italic> clinical isolates with drug resistance. The aim of this work was to evaluate the effect of lipase F (LipF) expression on mycobacterial drug resistance.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>The effects of expressing <jats:italic>lipF<\/jats:italic> from <jats:italic>Mycobacterium tuberculosis<\/jats:italic> in <jats:italic>Mycobacterium smegmatis<\/jats:italic> on resistance to antituberculosis drugs were determined with resazurin microtiter assay plate and growth kinetics. Functionality of ectopic LipF was confirmed. LipF expression reduced the rifampicin (RIF) and streptomycin (STR) minimum inhibitory concentration (MIC) from 3.12\u2009\u03bcg\/mL to 1.6\u2009\u03bcg\/mL and 0.25\u2009\u03bcg\/mL to 0.06\u2009\u03bcg\/mL respectively, moreover a reduced <jats:italic>M. smegmatis<\/jats:italic> growth in presence of RIF and STR compared with that of a control strain without LipF expression (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05 and <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01) was shown.<\/jats:p>\n<\/jats:sec><jats:sec>\n<jats:title>Conclusions<\/jats:title>\n<jats:p>LipF expression was associated with increased RIF and STR sensitivity in mycobacteria. Reduced LipF expression may contribute to the development of RIF and STR resistance in <jats:italic>Mycobacterium<\/jats:italic> species. Our findings provide information pertinent to understanding mycobacterial drug resistance mechanisms.<\/jats:p>\n<\/jats:sec>","DOI":"10.1186\/s12866-020-01802-x","type":"journal-article","created":{"date-parts":[[2020,5,25]],"date-time":"2020-05-25T12:02:43Z","timestamp":1590408163000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["LipF increases rifampicin and streptomycin sensitivity in a Mycobacterium tuberculosis surrogate"],"prefix":"10.1186","volume":"20","author":[{"given":"Ana Leticia","family":"Arriaga-Guerrero","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos E.","family":"Hern\u00e1ndez-Luna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joyce","family":"Rigal-Leal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene J.","family":"Robles-Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura Adiene","family":"Gonz\u00e1lez-Escalante","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Silva-Ram\u00edrez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Mercado-Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Vargas-Villarreal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Berm\u00fadez de Le\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9220-2421","authenticated-orcid":false,"given":"Katia","family":"Pe\u00f1uelas-Urquides","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,5,25]]},"reference":[{"key":"1802_CR1","volume-title":"Global Tuberculosis Report 2018","author":"WHO","year":"2018","unstructured":"WHO: Global Tuberculosis Report 2018. 2018."},{"issue":"5073","key":"1802_CR2","doi-asserted-by":"publisher","first-page":"1055","DOI":"10.1126\/science.257.5073.1055","volume":"257","author":"BR Bloom","year":"1992","unstructured":"Bloom BR, Murray CJ. 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