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This study applied a combination of long-read Oxford Nanopore MinION and short-read Illumina MiSeq-based sequencing to obtain closed complete genome sequences of two CTX-M-producing multidrug-resistant <jats:italic>Escherichia coli<\/jats:italic> strains isolated from blue mussels (<jats:italic>Mytilus edulis<\/jats:italic>) in Norway, in order to understand the potential for mobility of the detected antibiotic resistance genes (ARGs).<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The complete genome sequence of strain 631 (<jats:italic>E. coli<\/jats:italic> sequence type 38) was assembled into a circular chromosome of 5.19\u2009Mb and five plasmids (between 98\u2009kb and 5\u2009kb). The majority of ARGs cluster in close proximity to each other on the chromosome within two separate multidrug-resistance determining regions (MDRs), each flanked by IS<jats:italic>26<\/jats:italic> transposases. MDR-1 carries <jats:italic>bla<\/jats:italic><jats:sub>TEM-1<\/jats:sub>, <jats:italic>tmrB<\/jats:italic>, <jats:italic>aac(3)-IId<\/jats:italic>, <jats:italic>aadA5<\/jats:italic>, <jats:italic>mph(A)<\/jats:italic>, <jats:italic>mrx<\/jats:italic>, <jats:italic>sul1<\/jats:italic>, <jats:italic>qacE<\/jats:italic>\u03941 and <jats:italic>dfrA17<\/jats:italic>; while MDR-2 harbors <jats:italic>aph(3\u2033)-Ib<\/jats:italic>, <jats:italic>aph(6)-Id<\/jats:italic>, <jats:italic>bla<\/jats:italic><jats:sub>TEM-1<\/jats:sub>, <jats:italic>catA1<\/jats:italic>, <jats:italic>tet(D)<\/jats:italic> and <jats:italic>sul2<\/jats:italic>. Four identical chromosomal copies of <jats:italic>bla<\/jats:italic><jats:sub>CTX-M-14<\/jats:sub> are located outside these regions, flanked by IS<jats:italic>Ec9<\/jats:italic> transposases. Strain 1500 (<jats:italic>E. coli<\/jats:italic> sequence type 191) exhibited a circular chromosome of 4.73\u2009Mb and two plasmids (91\u2009kb and 4\u2009kb). The 91\u2009kb conjugative plasmid belonging to IncI1 group carries <jats:italic>bla<\/jats:italic><jats:sub>CTX-M-15<\/jats:sub> and <jats:italic>bla<\/jats:italic><jats:sub>TEM-1<\/jats:sub> genes.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>This study confirms the efficacy of combining Nanopore long-read and Illumina short-read sequencing for determining complete bacterial genome sequences, enabling detection and characterization of clinically important ARGs in the marine environment in Norway, with potential for further dissemination. It also highlights the need for environmental surveillance of antibiotic resistance in low prevalence settings like Norway.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-020-01821-8","type":"journal-article","created":{"date-parts":[[2020,5,25]],"date-time":"2020-05-25T17:03:13Z","timestamp":1590426193000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Nanopore sequencing reveals genomic map of CTX-M-type extended-spectrum \u03b2-lactamases carried by Escherichia coli strains isolated from blue mussels (Mytilus edulis) in Norway"],"prefix":"10.1186","volume":"20","author":[{"given":"Didrik H.","family":"Grevskott","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco","family":"Salv\u00e0-Serra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward R. 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