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Stool and urine samples were collected from both groups for 16S rRNA gene sequencing and metabolomic analysis.<\/jats:p>\n                <jats:p>The results showed that fecal microbiota in IBS-D featured depleted <jats:italic>Faecalibacterium<\/jats:italic> (adjusted <jats:italic>P\u2009=<\/jats:italic>\u00a00.034), <jats:italic>Eubacterium rectale<\/jats:italic> group (adjusted <jats:italic>P\u2009=<\/jats:italic>\u00a00.048), <jats:italic>Subdoligranulum<\/jats:italic> (adjusted <jats:italic>P\u2009=<\/jats:italic>\u00a00.041) and increased <jats:italic>Prevotella<\/jats:italic> (adjusted <jats:italic>P\u2009=<\/jats:italic>\u00a00.041). O-ureido-L-serine, 3,4-dihydroxybenzenesulfonic acid and (R)-2-Hydroxyglutarate demonstrated lower urinary concentrations in IBS-D patients. We further built correlation matrices between gut microbe abundance, differentiated metabolite quantities and clinical parameters. <jats:italic>Dialister<\/jats:italic> manifested negative association with IBS severity (<jats:italic>r<\/jats:italic>\u2009=\u2009\u2212\u20090.285, <jats:italic>P<\/jats:italic>\u2009=\u20090.017), anxiety (<jats:italic>r<\/jats:italic>\u2009=\u2009\u2212\u20090.347, <jats:italic>P<\/jats:italic>\u2009=\u20090.003) and depression level (<jats:italic>r<\/jats:italic>\u2009=\u2009\u2212\u20090.308, <jats:italic>P<\/jats:italic>\u2009=\u20090.010). <jats:italic>Roseburia<\/jats:italic> was negatively associated with IBS severity (<jats:italic>r<\/jats:italic>\u2009=\u2009\u2212\u20090.298, <jats:italic>P<\/jats:italic>\u2009=\u20090.012). Twenty metabolites correlated with anxiety or depression levels, including 3,4-dihydroxymandelaldehyde with SAS (<jats:italic>r<\/jats:italic>\u2009=\u2009\u2212\u20090.383, <jats:italic>P<\/jats:italic>\u2009=\u20090.001), 1-methylxanthine with SDS (<jats:italic>r<\/jats:italic>\u2009=\u2009\u2212\u20090.347, <jats:italic>P<\/jats:italic>\u2009=\u20090.004) and 1D-chiro-inositol with SAS (<jats:italic>r<\/jats:italic>\u2009=\u2009\u2212\u20090.336, <jats:italic>P<\/jats:italic>\u2009=\u20090.005). In analysis of microbe-metabolite relationship, 3,4-dihydroxymandelaldehyde and 1-methylxanthine were negatively correlated with relative abundance of <jats:italic>Clostridium<\/jats:italic><jats:italic>sensu stricto<\/jats:italic><jats:italic>.<\/jats:italic><\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Our findings demonstrated altered microbial and metabolomic profiles associated with clinically and psychological symptoms in IBS-D patients, which may provide insights for further investigations.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1186\/s12866-020-01841-4","type":"journal-article","created":{"date-parts":[[2020,6,17]],"date-time":"2020-06-17T13:03:53Z","timestamp":1592399033000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Microbial and metabolomic profiles in correlation with depression and anxiety co-morbidities in diarrhoea-predominant IBS patients"],"prefix":"10.1186","volume":"20","author":[{"given":"Tong","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Xiang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9556-8771","authenticated-orcid":false,"given":"Xiu-li","family":"Zuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,6,17]]},"reference":[{"key":"1841_CR1","doi-asserted-by":"publisher","first-page":"1075","DOI":"10.1136\/gutjnl-2015-311240","volume":"66","author":"AD Sperber","year":"2017","unstructured":"Sperber AD, Dumitrascu D, Fukudo S, Gerson C, Ghoshal UC, Gwee KA, et al. 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