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Observations of antibiotic-induced cell morphology changes can rapidly and accurately differentiate drug susceptible from resistant bacterial strains; however, resistant cells do not always remain unchanged.\n                      <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n                      is a Gram-negative, biothreat pathogen and the causative agent of melioidosis, an often fatal infectious disease for humans.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Here, we identified \u03b2-lactam targets in\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      by in silico analysis. Ten genes encoding putative PBPs, including PBP-1, PBP-2, PBP-3 and PBP-6, were detected in the genomes of susceptible and resistant strains. Real-time, live-cell imaging of\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      strains demonstrated dynamic morphological changes in broth containing clinically relevant \u03b2-lactam antibiotics. At sub-inhibitory concentrations of ceftazidime (CAZ), amoxicillin-clavulanic acid (AMC), and imipenem (IPM), filamentation, varying in length and proportion, was an initial response of the multidrug-resistant strain Bp1651 in exponential phase. However, a dominant morphotype reemerged during stationary phase that resembled cells unexposed to antibiotics. Similar morphology dynamics were observed for AMC-resistant strains, MSHR1655 and 724644, when exposed to sub-inhibitory concentrations of AMC. For all\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      strains evaluated, increased exposure time and exposure to increased concentrations of AMC at and above minimal inhibitory concentrations (MICs) in broth resulted in cell morphology shifts from filaments to spheroplasts and\/or cell lysis.\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      morphology changes were more consistent in IPM. Spheroplast formation followed by cell lysis was observed for all strains in broth containing IPM at concentrations greater than or equal to MICs, however, the time to cell lysis was variable.\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      cell lengths were strain-, drug- and drug concentration-dependent.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Both resistant and susceptible\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      strains exhibited filamentation during early exposure to AMC and CAZ at concentrations used to interpret susceptibility (based on CLSI guidelines). While developing a rapid \u03b2-lactam antimicrobial susceptibility test based on cell-shape alone requires more extensive analyses, optical microscopy detected\n                      <jats:italic>B. pseudomallei<\/jats:italic>\n                      growth attributes that lend insight into antibiotic response and antibacterial mechanisms of action.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12866-020-01865-w","type":"journal-article","created":{"date-parts":[[2020,7,16]],"date-time":"2020-07-16T05:02:46Z","timestamp":1594875766000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Optical microscopy reveals the dynamic nature of B. pseudomallei morphology during \u03b2-lactam antimicrobial susceptibility testing"],"prefix":"10.1186","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4155-5347","authenticated-orcid":false,"given":"Heather P.","family":"McLaughlin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Bugrysheva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Sue","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,7,16]]},"reference":[{"key":"1865_CR1","unstructured":"World Health Organization. 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