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This study investigated 475 captive and free-living bats from Switzerland, Germany, and Costa Rica for\n                      <jats:italic>Chlamydiales<\/jats:italic>\n                      and hemotropic mycoplasmas by PCR to determine the prevalence and phylogeny of these organisms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Screening for\n                      <jats:italic>Chlamydiales<\/jats:italic>\n                      resulted in a total prevalence of 31.4%. Positive samples originated from captive and free-living bats from all three countries. Sequencing of 15 samples allowed the detection of two phylogenetically distinct groups. These groups share sequence identities to\n                      <jats:italic>Chlamydiaceae<\/jats:italic>\n                      , and to\n                      <jats:italic>Chlamydia<\/jats:italic>\n                      -like organisms including\n                      <jats:italic>Rhabdochlamydiaceae<\/jats:italic>\n                      and unclassified\n                      <jats:italic>Chlamydiales<\/jats:italic>\n                      from environmental samples, respectively.\n                    <\/jats:p>\n                    <jats:p>PCR analysis for the presence of hemotropic mycoplasmas resulted in a total prevalence of 0.7%, comprising free-living bats from Germany and Costa Rica. Phylogenetic analysis revealed three sequences related to other unidentified mycoplasmas found in vampire bats and Chilean bats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Bats can harbor\n                      <jats:italic>Chlamydiales<\/jats:italic>\n                      and hemotropic mycoplasmas and the newly described sequences in this study indicate that the diversity of these bacteria in bats is much larger than previously thought. Both,\n                      <jats:italic>Chlamydiales<\/jats:italic>\n                      and hemotropic mycoplasmas are not restricted to certain bat species or countries and captive and free-living bats can be colonized. In conclusion, bats represent another potential host or vector for novel, previously unidentified,\n                      <jats:italic>Chlamydiales<\/jats:italic>\n                      and hemotropic mycoplasmas.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1186\/s12866-020-01872-x","type":"journal-article","created":{"date-parts":[[2020,6,26]],"date-time":"2020-06-26T01:02:44Z","timestamp":1593133364000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Prevalence and phylogeny of Chlamydiae and hemotropic mycoplasma species in captive and free-living bats"],"prefix":"10.1186","volume":"20","author":[{"given":"Janine","family":"Fritschi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanna","family":"Marti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena M. 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